Information on EC 2.7.1.108 - dolichol kinase

Word Map on EC 2.7.1.108
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.1.108
-
RECOMMENDED NAME
GeneOntology No.
dolichol kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
CTP + dolichol = CDP + dolichyl phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
Phosphorylation
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
dolichol and dolichyl phosphate biosynthesis
-
-
Metabolic pathways
-
-
N-Glycan biosynthesis
-
-
dolichol and dolichyl phosphate biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
CTP:dolichol O-phosphotransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
71768-07-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene At3g45040 or DOK1
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
soybean
-
-
Manually annotated by BRENDA team
Wistar
-
-
Manually annotated by BRENDA team
strain SF402-4D (Ferro-Novick et al., 1984), temperature sensitive mutants, mutants impaired in Sec59p (sec59-1), cells are defective in CTP-dependent dolichol kinase
-
-
Manually annotated by BRENDA team
rye
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CTP + dolichol
CDP + dolichyl phosphate
show the reaction diagram
CTP + ficaprenol
CDP + ficaprenyl phosphate
show the reaction diagram
CTP + polyprenol
CDP + polyprenyl phosphate
show the reaction diagram
dCTP + dolichol
dCDP + dolichyl phosphate
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CTP + dolichol
CDP + dolichyl phosphate
show the reaction diagram
additional information
?
-
-
the chain length of eukaryotic dolichol molecules is species specific and differs from 14-17 isoprene units in unicellular organisms like the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, mammalian cells produce longer dolichol molecules with 18-21 isoprene units
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
-
slight activation
Fe2+
-
slight activation
Ni2+
-
slight activation
Sr2+
-
slight activation
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
-
above 6 mM
chlorpromazine
-
-
Co2+
-
above 6 mM
dCDP
-
-
Mn2+
-
above 6 mM
Trifluoperazine
-
-
Triton X-100
-
above 0.5% w/v, activation below
Zn2+
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(R)-dolichol (C95)
-
markedly stimulated by addition, Km 0.009 mM
(S)-dolichol (C95)
-
markedly stimulated by addition, Km 0.009 mM
2-mercaptoethanol
-
slight stimulation
dimyristoylphosphatidylcholine
-
stimulatory in 1:1 ratio with deoxycholate
dithiothreitol
-
slight stimulation of activity
monothioglycerol
-
slight stimulation
phosphatidylcholine
-
stimulated by after dialysis
phosphatidylethanolamine
-
stimulated by after dialysis
Triton X-100
additional information
-
sodium dodecylsulfate is ineffective in stimulating enzymic activity
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0065 - 4
CTP
0.0091
dCTP
-
pH 7.2, 37C
0.023 - 0.075
dolichol
additional information
additional information
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.121
CDP
-
pH 7.2, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.15
-
relative specific activity in the soluble fraction
0.21
-
relative specific activity in the mitochondrial lysosomal fraction
0.52
-
relative specific activity in the nuclear fraction
3.8
-
relative specific activity in the microsomal fraction, rough and smooth endoplasmic reticulum are found to have higher specific activities than the Golgi fraction
additional information
-
extracts gained from patient fibroblasts show remarkably lower enzyme activity than extracts from control cells, in comparison with controls, the decrease in activity was 94.5-98.6% for all patients
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8
-
second optimum at pH 9.5
7.4 - 7.7
-
-
7.4
-
Tris/maleate buffer, second optimum at pH 9.0
7.5
-
-
8
-
shoulder at pH 6.5
8.5
-
-
9
-
glycine buffer, second optimum at pH 7.4
9.5
-
second optimum at pH 7.0-8.0
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 11
-
no second optimum with Tris-HCl, activity increases fairly linearly between pH 8.0 and 10.0, activity decreases about 25% between pH 9.5 and 10.0 in Chaps buffer
4 - 10
-
about half-maximal activity at pH 6.0 and 8.0
6.6 - 10.2
-
broad pH-activity profile with good activity observed from pH 7.0-10.0, about half-maximal activity at pH 6.6 and 10.2
6.6 - 9.6
-
2 optima, about half-maximal activity at pH 6.6, pH 8.5 and pH 9.6
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 37
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
DOK1 is highly expressed in tapetum cells and microspores during early anther development
Manually annotated by BRENDA team
high expression level
Manually annotated by BRENDA team
root tip of germinating seedlings, and in the founder cells where lateral root growth is initiated
Manually annotated by BRENDA team
additional information
gene AtDOK1 is expressed in specific cell types involved in meristematic activities, analysis of tissue-specific expression of DOK1 by histochemical GUS staining, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
polytopic membrane protein localized in the endoplasmic reticulum with an N-terminus extended into the lumen and a cytoplasmically oriented C-terminus
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
59000 - 60000
-
SDS-PAGE
59270
-
predicted from cDNA open reading frame
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-17C, 65% of original activity retains when stored for 6 weeks
-
-17C, stable for at least 1 month
-
-20C, stable for at least 15 days
-
-20C, stored frozen as pellet for months with little loss of activity
-
4C, no detectable loss of activity when solubilized extract is kept for 24 h
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partially
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene At3g45040 or AtDOK1, DNA and amino acid sequence determination and analysis, a cytidylyltransferase motif-containing gene, phylogenetic tree. The gene complements defects in the growth and N-linked glycosylation of the Saccharomyces cerevisiae sec59 mutant, deficient in dolichol kinase Sec59p
gene dolk, genotyping and identification of DOLK mutations
human cDNA complements defect in dolichol kinase activity in Saccharomyces cerevisiae sec59-1 mutant, human SEC59 gene also expressed in Sf-9 cells
-
overexpression of normal or variant forms of hDK in CHO cells and in yeast sec59-1 cells
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Q483K
naturally occuring homozygous c.1447C>A DOLK mutation involved in enzyme deficiency and a phenotype with anatomic malformations and multi-systemic dysfunction
additional information
construction of two independent lines of T-DNA-tagged AtDOK1 mutants, designated dok1-1 (CS822420; SAIL_529_E06) and dok1-2 (CS856101; WiscDsLox443D6), with T-DNA insertions in exons 8 and 13, respectively. Mutant dok1-1/+ and wild-type seed morphologies show no significant differences, and dok1-1/+ and the wild type did not differ in seed germination rates