Information on EC 2.7.1.102 - hamamelose kinase

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The expected taxonomic range for this enzyme is: Kluyvera cryocrescens

EC NUMBER
COMMENTARY hide
2.7.1.102
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RECOMMENDED NAME
GeneOntology No.
hamamelose kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + D-hamamelose = ADP + D-hamamelose 2'-phosphate
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
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SYSTEMATIC NAME
IUBMB Comments
ATP:D-hamamelose 2'-phosphotransferase
Also acts, more slowly, on D-hamamelitol.
CAS REGISTRY NUMBER
COMMENTARY hide
74506-53-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain 627, grown on D-hamamelose
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Manually annotated by BRENDA team
strain 627, grown on D-hamamelose
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + D-hamamelitol
ADP + D-hamamelitol 2'-phosphate
show the reaction diagram
ATP + D-hamamelose
ADP + hamamelose 2'-phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + D-hamamelose
ADP + hamamelose 2'-phosphate
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
-
above 7 mM: significant substrate inhibition
hamamelose
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above 5 mM: significant substrate inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
enzyme is induced by growing on D-hamamelose
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.5
ATP
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at 30°C, pH 7.5
3
D-hamamelose
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at 30°C, pH 7.5
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.6 - 6.6
-
at 30°C, pH 7.5
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.6 - 8.5
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10% of maximal activity at pH 6.6, 80% of maximal activity at pH 8.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
21000
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gel filtration
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.4
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optimal enzyme stability in the crude extract at
640277
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
no stabilization by dithioerythritol, mercaptoethanol, glycerol or bovine serum albumin
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purified enzyme is very labile, 4 mM D-hamamelose, added to storage buffer, stabilizes
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, purified enzyme, within 10 days, complete loss of activity
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low temperatures, 4 mM hamamelose, protein concentration not lower than 0.2 mg/ml, stable
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial, 51fold
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