Information on EC 2.6.1.B3 - aminopentol:pyruvate aminotransferase FumI

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.6.1.B3
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
aminopentol:pyruvate aminotransferase FumI
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2S,3S,5R,10S,12R,14R,15S,16S)-2-amino-12,16-dimethylicosane-3,5,10,14,15-pentol + pyruvate = (3S,5R,10S,12R,14R,15S,16S)-3,5,10,14,15-pentahydroxy-12,16-dimethylicosan-2-one + L-alanine
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
(2S,3S,5R,10S,12R,14R,15S,16S)-2-amino-12,16-dimethylicosane-3,5,10,14,15-pentol:pyruvate aminotransferase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Sphingopyxis sp.
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UniProt
Manually annotated by BRENDA team
ATCC 55552
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2S,3S,5R,10S,12R,14R,15S,16S)-2-amino-12,16-dimethylicosane-3,5,10,14,15-pentol + oxaloacetate
(3S,5R,10S,12R,14R,15S,16S)-3,5,10,14,15-pentahydroxy-12,16-dimethylicosan-2-one + L-aspartate
show the reaction diagram
(2S,3S,5R,10S,12R,14R,15S,16S)-2-amino-12,16-dimethylicosane-3,5,10,14,15-pentol + pyruvate
(3S,5R,10S,12R,14R,15S,16S)-3,5,10,14,15-pentahydroxy-12,16-dimethylicosan-2-one + L-alanine
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(2S,3S,5R,10S,12R,14R,15S,16S)-2-amino-12,16-dimethylicosane-3,5,10,14,15-pentol + pyruvate
(3S,5R,10S,12R,14R,15S,16S)-3,5,10,14,15-pentahydroxy-12,16-dimethylicosan-2-one + L-alanine
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2S,3S,5R,10S,12R,14R,15S,16S)-2-amino-12,16-dimethylicosane-3,5,10,14,15-pentol
Sphingopyxis sp.
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substrate inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
Sphingopyxis sp.
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-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0011
(2S,3S,5R,10S,12R,14R,15S,16S)-2-amino-12,16-dimethylicosane-3,5,10,14,15-pentol
Sphingopyxis sp.
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pH 8.0, 35C
4.15
oxaloacetate
Sphingopyxis sp.
-
pH 8.0, 35C
0.49
pyruvate
Sphingopyxis sp.
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pH 8.0, 35C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.73
(2S,3S,5R,10S,12R,14R,15S,16S)-2-amino-12,16-dimethylicosane-3,5,10,14,15-pentol
Sphingopyxis sp.
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pH 8.0, 35C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.2
Sphingopyxis sp.
-
pH 8.0, 35C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.25
Sphingopyxis sp.
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sharp optimum at pH 8.0 and 8.5
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9.7
Sphingopyxis sp.
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-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
Sphingopyxis sp.
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-
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 45
Sphingopyxis sp.
-
-
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.48
calculated from sequence
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48270
Sphingopyxis sp.
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calculated
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Sphingopyxis sp.
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activity reduced at pH 6.5 or less (1 h, 35C), activity reduced at pH 8.0 (1 h)
724058
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
Sphingopyxis sp.
-
rapid inactivation at 50C
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 25% glycerol, stable for several month
Sphingopyxis sp.
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
His-tagged protein: immobilized metal ion affinity chromatography (Ni2+), maltose binding protein fusion protein: affinity chromatography (dextrin sepharose), cleavage of the fusion protein
Sphingopyxis sp.
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immobilized metal ion affinity chromatography (Ni2+)
Sphingopyxis sp.
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
His-tagged protein and maltose binding protein fusion protein expressed in different Escherichia coli (DE3) strains at different temeratures and different expression levels
Sphingopyxis sp.
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His-tagged protein expressed in Escherichia coli ArcticExpress (DE3), His-tag does not affect the enzymatic activity
Sphingopyxis sp.
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
food industry
enzymatic detoxification of fumonisins in animal feed and potentially also in foodstuffs intended for human consumption, improvement of food and feed safety