Information on EC 2.6.1.99 - L-tryptophan-pyruvate aminotransferase

Word Map on EC 2.6.1.99
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.6.1.99
-
RECOMMENDED NAME
GeneOntology No.
L-tryptophan-pyruvate aminotransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-tryptophan + pyruvate = indole-3-pyruvate + L-alanine
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
indole-3-acetate biosynthesis I
-
-
indole-3-acetate biosynthesis II
-
-
indolmycin biosynthesis
-
-
Metabolic pathways
-
-
Tryptophan metabolism
-
-
tryptophan metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
L-tryptophan:pyruvate aminotransferase
This plant enzyme, along with EC 1.14.13.168, indole-3-pyruvate monooxygenase, is responsible for the biosynthesis of the plant hormone indole-3-acetate from L-tryptophan.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Oryza sativa susp. japonica
-
-
-
Manually annotated by BRENDA team
gene vanishing tassel2, vt2
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-isoleucine + indole-3-pyruvic acid
3-methyl-2-oxopentanoate + L-tryptophan
show the reaction diagram
1% of the activity with L-methionine
-
-
?
L-leucine + indole-3-pyruvic acid
2-oxo-4-methylpentanoate + L-tryptophan
show the reaction diagram
1% of the activity with L-methionine
-
-
?
L-methionine + indole-3-pyruvic acid
2-oxo-4-methylthiobutanoate + L-tryptophan
show the reaction diagram
most catalytically preferred amino donor. No reverse VAS1 activity detected
-
-
i
L-phenylalanine + indole-3-pyruvic acid
phenylpyruvate + L-tryptophan
show the reaction diagram
21% of the activity with L-methionine
-
-
?
L-tryptophan + pyruvate
indole-3-pyruvate + L-alanine
show the reaction diagram
L-tyrosine + indole-3-pyruvic acid
3-(4-hydroxyphenyl)-2-oxopropanoate + L-tryptophan
show the reaction diagram
1% of the activity with L-methionine
-
-
?
L-valine + indole-3-pyruvic acid
3-methyl-2-oxobutanoate + L-tryptophan
show the reaction diagram
1% of the activity with L-methionine
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-tryptophan + pyruvate
indole-3-pyruvate + L-alanine
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2S)-2-(aminooxy)-3-(naphthalen-2-yl)propanoic acid
-
competitive. Arabidopsis thaliana seedlings treated with the compound show typical auxin-deficient phenotypes, which are reversed by exogenous indole-3-acetic acid
L-alpha-aminooxy-phenylpropionic acid
-
target compound for inhibitor synthesis
L-kynurenine
additional information
-
synthesis of inhibitors based on L-alpha-aminooxy-phenylpropionic acid. The aminooxy and carboxy groups of the compound are essential for inhibition of TAA1 in vitro. Inhibitory activity of the compounds is correlated with their binding energy with TAA1. The active compounds reduce the endogenous indole-3-acetic acid content upon application to Arabidopsis thaiana seedlings
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01152
L-kynurenine
-
inhibition of TAA1, competitive versus L-Tryp, pH not specificied in the publication, 55°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000077
(2S)-2-(aminooxy)-3-(naphthalen-2-yl)propanoic acid
Arabidopsis thaliana
-
pH 8.5, 35°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
of axillary meristems
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
in the inflorescence stem
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
molecular docking of inhibitors (2S)-2-(aminooxy)-3-(naphthalen-2-yl)propanoic acid and L-alpha-aminooxy-phenylpropionic acid
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression of GST-TAA1 in Escherichia coli strain BL21 Star (DE3) pLysS
-
gene sav3, DNA and amino acid sequence deterination and analysis, SAV3 has a localized and dynamic expression pattern
-
gene vt2, DNA and amino acid sequence determination and analysis, positional cloning of vt2, phylogenetic analysis
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K217A
-
the TAA1 mutant shows a defect in pyridoxal 5'-phosphate binding
P166S
-
wei8-2 utant version of TAA1
K217A
-
the TAA1 mutant shows a defect in pyridoxal 5'-phosphate binding
-
P166S
-
wei8-2 utant version of TAA1
-
additional information