Information on EC 2.6.1.95 - neomycin C transaminase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Streptomyces fradiae

EC NUMBER
COMMENTARY hide
2.6.1.95
-
RECOMMENDED NAME
GeneOntology No.
neomycin C transaminase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
neomycin C + 2-oxoglutarate = 6'''-deamino-6'''-oxoneomycin C + L-glutamate
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
neomycin biosynthesis
-
-
Neomycin, kanamycin and gentamicin biosynthesis
-
-
Biosynthesis of antibiotics
-
-
SYSTEMATIC NAME
IUBMB Comments
2-oxoglutarate:neomycin C aminotransferase
The reaction occurs in vivo in the opposite direction. Involved in the biosynthetic pathway of aminoglycoside antibiotics of the neomycin family. Works in combination with EC 1.1.3.44, 6'''-hydroxyneomycin C oxidase, to replace the 6'''-hydroxy group of 6'''-deamino-6'''-hydroxyneomycin C with an amino group. The enzyme, characterized from the bacterium Streptomyces fradiae, can also catalyse EC 2.6.1.93, neamine transaminase.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
neomycin + 2-oxoglutarate
6'''-deamino-6'''-dehydro-6'''-oxoneomycin + L-glutamate
show the reaction diagram
neomycin C + 2-oxoglutarate
6'''-hydroxyneomycin C + L-glutamate
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
NeoB is a bifunctional enzyme that also catalyses the NH2 group transfer at the 6'''-position
-
-
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48060
-
Neo-18, calculated from amino acid sequence; Neo-18, electrospray ionization mass spectrometry
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni2+-NTA resin column chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
-
expressed in Escherichia coli BL21(DE3) cells
-