Information on EC 2.6.1.93 - neamine transaminase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.6.1.93
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RECOMMENDED NAME
GeneOntology No.
neamine transaminase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
neamine + 2-oxoglutarate = 6'-dehydroparomamine + L-glutamate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
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kanamycin biosynthesis
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Neomycin, kanamycin and gentamicin biosynthesis
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ribostamycin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
neamine:2-oxoglutarate aminotransferase
The reaction occurs in vivo in the opposite direction. Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin B, butirosin, neomycin and ribostamycin. Works in combination with EC 1.1.3.43, paromamine 6-oxidase, to replace the 6'-hydroxy group of paromamine with an amino group. The enzyme from the bacterium Streptomyces kanamyceticus can also catalyse EC 2.6.1.94, 2'-deamino-2'-hydroxyneamine transaminase, which leads to production of kanamycin A [3]. The enzyme from the bacterium Streptomyces fradiae can also catalyse EC 2.6.1.95, leading to production of neomycin C [2].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
6'-dehydroparomamine + L-glutamate
neamine + 2-oxoglutarate
show the reaction diagram
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48060
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Neo-18, calculated from amino acid sequence; Neo-18, electrospray ionization mass spectrometry
52070
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BtrB, calculated from amino acid sequence; BtrB, electrospray ionization mass spectrometry
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni2+-NTA resin column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
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expressed in Escherichia coli BL21(DE3) cells
expressed in Streptomyces venezuelae strain PAR
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