Information on EC 2.6.1.77 - taurine-pyruvate aminotransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.6.1.77
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RECOMMENDED NAME
GeneOntology No.
taurine-pyruvate aminotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
taurine + pyruvate = L-alanine + 2-sulfoacetaldehyde
show the reaction diagram
; first step; The enzyme from Bilophila wadsworthia requires pryidoxal 5'-phosphate as a cofactor, is reversible, and catalyses the first step of anaerobic taurine degradation. Hypotaurine (i.e. 2-aminoethanesulfinate) and beta-alanine are also significant donors of an amino group. Unlike, EC 2.6.1.55, taurine-2-oxoglutarate transaminase, 2-oxoglutarate is not an acceptor of amino groups
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
transamination
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
hypotaurine degradation
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Metabolic pathways
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Taurine and hypotaurine metabolism
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taurine degradation I
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SYSTEMATIC NAME
IUBMB Comments
taurine:pyruvate aminotransferase
The enzyme from the bacterium Bilophila wadsworthia requires pyridoxal 5'-phosphate as a cofactor, and catalyses a reversible reaction that starts an anaerobic taurine degradation pathway. beta-Alanine is also a significant amino group donor. The enzyme from the bacterium Pseudomonas denitrificans PD1222 can also use hypotaurine, producing 2-sulfinoacetaldehyde, which spontaneously hydrolyses to sulfite and acetaldehyde. Unlike, EC 2.6.1.55, taurine---2-oxoglutarate transaminase, 2-oxoglutarate cannot serve as an acceptor for the amino group.
CAS REGISTRY NUMBER
COMMENTARY hide
51901-18-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
RZATAU
SwissProt
Manually annotated by BRENDA team
inducible by taurine; NKNTAU
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Manually annotated by BRENDA team
strain C1, only in induced, taurine-grown bacteria
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Manually annotated by BRENDA team
strain C1, only in induced, taurine-grown bacteria
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Manually annotated by BRENDA team
strain TauN1
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Manually annotated by BRENDA team
strain TauN1
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Manually annotated by BRENDA team
no activity in Clostridium pasteurianum
strain ATCC 6013
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Manually annotated by BRENDA team
no activity in Clostridium sp.
strain MS-1
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Manually annotated by BRENDA team
no activity in Clostridium sp. MS-1
strain MS-1
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Manually annotated by BRENDA team
no activity in Klebsiella oxytoca
strain M5a1
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Manually annotated by BRENDA team
no activity in Klebsiella oxytoca M5a1
strain M5a1
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Manually annotated by BRENDA team
no activity in Klebsiella sp.
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Manually annotated by BRENDA team
strain TAU-5
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Manually annotated by BRENDA team
orf459, expression inducible by taurine
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Manually annotated by BRENDA team
strain Tau3, inducible enzyme
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Manually annotated by BRENDA team
strain Tau3, inducible enzyme
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Manually annotated by BRENDA team
inducible enzyme
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Manually annotated by BRENDA team
of the tauR-ald-tpa gene cluster; strain ISO-5, inducible enzyme
SwissProt
Manually annotated by BRENDA team
of the tauR-ald-tpa gene cluster; strain ISO-5, inducible enzyme
SwissProt
Manually annotated by BRENDA team
strain Tau1, inducible enzyme
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Manually annotated by BRENDA team
strain Tau1, inducible enzyme
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Manually annotated by BRENDA team
DSS-3
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-1-methylbenzylamine + pyruvate
2-sulfoacetaldehyde + L-alanine
show the reaction diagram
1,4-diaminobutane + pyruvate
? + L-alanine
show the reaction diagram
1,6-diaminohexane + pyruvate
? + L-alanine
show the reaction diagram
1,7-diaminoheptane + pyruvate
? + L-alanine
show the reaction diagram
1-amylamine + pyruvate
pentanaldehyde + L-alanine
show the reaction diagram
1-butylamine + pyruvate
4-aminobutyraldehyde + L-alanine
show the reaction diagram
3-aminopropanesulfonate + pyruvate
?
show the reaction diagram
beta-alanine + pyruvate
3-oxopropanoate + L-alanine
show the reaction diagram
beta-alanine + pyruvate
?
show the reaction diagram
cysteine + pyruvate
?
show the reaction diagram
hypotaurine + pyruvate
2-aminoethanesulfinic acid + L-alanine
show the reaction diagram
hypotaurine + pyruvate
?
show the reaction diagram
spermidine + pyruvate
? + L-alanine
show the reaction diagram
spermine + pyruvate
? + L-alanine
show the reaction diagram
taurine + 2-oxobutanoate
2-sulfoacetaldehyde + 2-aminobutanoate
show the reaction diagram
taurine + 2-oxobutyrate
2-sulfoacetaldehyde + 2-aminobutyrate
show the reaction diagram
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?
taurine + 2-oxoglutarate
2-sulfoacetaldehyde + L-glutamate
show the reaction diagram
taurine + butanal
2-sulfoacetaldehyde + butyl-1-amine
show the reaction diagram
taurine + ethanal
2-sulfoacetaldehyde + ethylamine
show the reaction diagram
taurine + glycolaldehyde
2-sulfoacetaldehyde + ?
show the reaction diagram
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?
taurine + glyoxylate
2-sulfoacetaldehyde + glycine
show the reaction diagram
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?
taurine + hexanal
2-sulfoacetaldehyde + hexyl-1-amine
show the reaction diagram
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?
taurine + methanal
2-sulfoacetaldehyde + methylamine
show the reaction diagram
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?
taurine + oxaloacetate
?
show the reaction diagram
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?
taurine + pentanal
2-sulfoacetaldehyde + pentyl-1-amine
show the reaction diagram
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?
taurine + propanal
2-sulfoacetaldehyde + propyl-1-amine
show the reaction diagram
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?
taurine + pyruvate
2-sulfoacetaldehyde + L-alanine
show the reaction diagram
taurine + pyruvate
L-alanine + 2-sulfoacetaldehyde
show the reaction diagram
taurine + pyruvate
sulfoacetaldehyde + L-alanine
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
taurine + pyruvate
2-sulfoacetaldehyde + L-alanine
show the reaction diagram
Q5LVM7
involved in taurine catabolism
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?
taurine + pyruvate
L-alanine + 2-sulfoacetaldehyde
show the reaction diagram
taurine + pyruvate
sulfoacetaldehyde + L-alanine
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
additional information
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
L-alanine
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feed-back inhibition, no growth of the organism in presence of L-alanine
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8.1
Hypotaurine
pH 9.0, 35°C
0.82
pyruvate
pH 9.0, 35°C
5.3 - 7.1
taurine
additional information
additional information
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.065
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mutant TR-3R, pH 8.0, 30°C
0.07
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pH 8.0, 30°C
0.26
substrate (S)-1-methylbenzylamine, pH 9.0, 37°C
0.68
substrate butanal, pH 9.0, 37°C; substrate ethanal, pH 9.0, 37°C
1.02
taurine/formate-grown cells
2.22
pyruvate-grown cells
35.3
purified enzyme
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 9.5
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broad optimal range, no activity at pH 10.5
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 40
broad optimum
25 - 37
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no activity at 44°C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
effectively absent in acetate grown cells, also absent in cysteate-grown cells
Manually annotated by BRENDA team
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000
x * 50000, SDS-PAGE
197000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
molecular modeling of activte site. The active site shows a special large pocket and substrate tunnel
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
enzyme is unstable in buffers with neutral or alkaline pH value, except for HEPES at pH 7.0
640197
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
glycerol stabilizes
pyridoxal 5'-phosphate may stabilize the enzyme
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Methanol
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C or 22°C, sulfonate buffers MES or MOPS, pH range 6.0-7.0, 22 h, stable
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
buffer MOPS, pH 6.5, apparent homogeneity
partially by ultracentrifugation and anion exchange chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination and analysis
gene cluster tauR-ald-tpa, genetic organization
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gene cluster tauR-ald-tpa, genetic organization, DNA and amino acid sequence determination and analysis
orf459, essential for activity, no enzyme activity in deletion mutants
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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identification of Bilophila wadsworthia by specific PCR which targets the taurine:pyruvate aminotransferase gene
Show AA Sequence (423 entries)
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