Information on EC 2.6.1.76 - diaminobutyrate-2-oxoglutarate transaminase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.6.1.76
-
RECOMMENDED NAME
GeneOntology No.
diaminobutyrate-2-oxoglutarate transaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-2,4-diaminobutanoate + 2-oxoglutarate = L-aspartate 4-semialdehyde + L-glutamate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
rhizobactin 1021 biosynthesis
-
-
pyoverdine I biosynthesis
-
-
ectoine degradation
-
-
ectoine biosynthesis
-
-
norspermidine biosynthesis
-
-
threonine metabolism
-
-
Glycine, serine and threonine metabolism
-
-
Metabolic pathways
-
-
Microbial metabolism in diverse environments
-
-
SYSTEMATIC NAME
IUBMB Comments
L-2,4-diaminobutanoate:2-oxoglutarate 4-aminotransferase
A pyridoxal-phosphate protein that requires potassium for activity [4]. In the proteobacterium Acinetobacter baumannii, this enzyme is cotranscribed with the neighbouring ddc gene that also encodes EC 4.1.1.86, diaminobutyrate decarboxylase. Differs from EC 2.6.1.46, diaminobutyrate---pyruvate transaminase, which has pyruvate as the amino-group acceptor. This is the first enzyme in the ectoine-biosynthesis pathway, the other enzymes involved being EC 2.3.1.178, diaminobutyrate acetyltransferase and EC 4.2.1.108, ectoine synthase [3,4].
CAS REGISTRY NUMBER
COMMENTARY hide
196622-96-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
3 ATCC 19004
-
-
Manually annotated by BRENDA team
ATCC 17906
-
-
Manually annotated by BRENDA team
ATCC 17909
-
-
Manually annotated by BRENDA team
ATCC 17908
-
-
Manually annotated by BRENDA team
ATCC 15309
-
-
Manually annotated by BRENDA team
IAM13186
-
-
Manually annotated by BRENDA team
Rd, types a, b, d, e, f
-
-
Manually annotated by BRENDA team
CGMCC 1.6329
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-aminobutanoate + 2-oxoglutarate
? + L-glutamate
show the reaction diagram
about 60% of the activity with L-2,4-diaminobutanoate
-
-
r
beta-alanine + 2-oxoglutaric acid
?
show the reaction diagram
-
-
-
-
?
L-2,4-diaminobutanoate + 2-oxoglutarate
L-aspartate 4-semialdehyde + L-glutamate
show the reaction diagram
L-2,4-diaminobutyric acid + 2-oxoglutaric acid
L-glutamic acid + L-aspartic beta-semialdehyde
show the reaction diagram
L-aspartate 4-semialdehyde + L-glutamate
L-2,4-diaminobutanoate + 2-oxoglutarate
show the reaction diagram
-
-
-
r
L-aspartate 4-semialdehyde + L-homoserine
L-2,4-diaminobutanoate + 4-hydroxy-2-oxobutanoic acid
show the reaction diagram
about 5% of the activity with L-glutamate
-
-
r
L-glutamic acid + L-aspartic beta-semialdehyde
L-2,4-diaminobutyric acid + 2-oxoglutaric acid
show the reaction diagram
L-lysine + oxaloacetic acid
?
show the reaction diagram
-
-
-
-
?
L-ornithine + 2-oxoglutarate
? + L-glutamate
show the reaction diagram
about 6% of the activity with L-2,4-diaminobutanoate
-
-
r
L-ornithine + pyruvic acid
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
no substrates: L-alanine, L-aspartate, L-lysine
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.21 - 1.07
2-oxoglutarate
1.46
2-oxoglutaric acid
-
-
0.04 - 0.09
L-2,4-diaminobutanoate
4.3
L-2,4-diaminobutyric acid
-
-
4.5
L-aspartate 4-semialdehyde
pH 8.5, 25°C
9.1
L-glutamate
pH 8.5, 25°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.5 - 25.2
2-oxoglutarate
120 - 221
L-2,4-diaminobutanoate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4 - 120
2-oxoglutarate
1333 - 5535
L-2,4-diaminobutanoate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
12
pH 8.5, 25°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2
isoelectric focussing
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44000
6 * 44000, SDS-PAGE
45000
-
4 * 45000, SDS-PAGE
47420
-
deduced from nucleotide sequence
49400
-
predicted from amino acid sequence, gene HI0949
56200
-
predicted from amino acid sequence, gene HI946
188000
-
native enzyme, gel filtration
260000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
6 * 44000, SDS-PAGE
tetramer
-
4 * 45000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme, overexpressed in Escherichia coli
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Escherichia coli clone carrying the dat homolog of H. influenzae
-
homologs of dat detected by PCR amplification
identification and analysis of the dat gene encoding L-2,4-diaminobutyrate:2-ketoglutarate 4-aminotransferase with Escherichia coli transformants
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D180V
about 2fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant
D180V/E36V
double mutant displays less activity than mutant D180V
D180V/F320Y/Q325R
about 2.1-3.3fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant
E36V
about 1.5fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant
D180V
-
about 2fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant
-
D180V/E36V
-
double mutant displays less activity than mutant D180V
-
D180V/F320Y/Q325R
-
about 2.1-3.3fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant
-
E36V
-
about 1.5fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
synthesis
Show AA Sequence (1769 entries)
Please use the Sequence Search for a specific query.