Information on EC 2.6.1.73 - methionine-glyoxylate transaminase

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The expected taxonomic range for this enzyme is: Brassica

EC NUMBER
COMMENTARY hide
2.6.1.73
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RECOMMENDED NAME
GeneOntology No.
methionine-glyoxylate transaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-methionine + glyoxylate = 4-methylthio-2-oxobutanoate + glycine
show the reaction diagram
L-glutamate can also act as donor
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-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
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-
-
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SYSTEMATIC NAME
IUBMB Comments
L-methionine:glyoxylate aminotransferase
L-Glutamate can also act as donor.
CAS REGISTRY NUMBER
COMMENTARY hide
116155-75-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
var. R-4218
-
-
Manually annotated by BRENDA team
var. Topas
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-glutamine + 4-methylsulfanyl-2-oxobutanoate
2-oxoglutarate + L-methionine
show the reaction diagram
L-glutamine + glyoxylate
2-oxoglutarate + glycine
show the reaction diagram
L-histidine + glyoxylate
3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
show the reaction diagram
-
16% of activity compared to L-methionine
-
-
?
L-homoserine + glyoxylate
4-hydroxy-2-oxobutanoate + glycine
show the reaction diagram
-
56% of activity compared to L-methionine
-
-
?
L-methionine + 2-oxoglutarate
4-methylsulfanyl-2-oxobutanoate + L-glutamate
show the reaction diagram
-
19% of activity compared to glyoxylate
-
-
?
L-methionine + 4-methylsulfanyl-2-oxobutanoate
4-methylsulfanyl-2-oxobutanoate + L-methionine
show the reaction diagram
L-methionine + glyoxylate
4-methylsulfanyl-2-oxobutanoate + glycine
show the reaction diagram
L-methionine + oxaloacetate
4-methylsulfanyl-2-oxobutanoate + L-aspartate
show the reaction diagram
L-methionine + pyruvate
4-methylsulfanyl-2-oxobutanoate + L-alanine
show the reaction diagram
-
35% of activity compared to glyoxylate
-
-
?
L-methionine sulfone + glyoxylate
4-methylsulfonyl-2-oxobutanoate + glycine
show the reaction diagram
-
29% of activity compared to L-methionine
-
-
?
L-methionine sulfoxide + glyoxylate
4-methylsulfinyl-2-oxobutanoate + glycine
show the reaction diagram
-
54% of activity compared to L-methionine
-
-
?
L-methionine sulfoximine + glyoxylate
(S-methylsulfonimidoyl)-2-oxobutanoate + glycine
show the reaction diagram
-
35% of activity compared to L-methionine
-
-
?
S-methyl-L-cysteine + glyoxylate
3-methylsulfanyl-2-oxopropanoate + glycine
show the reaction diagram
-
49% of activity compared to L-methionine
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.06
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isoenzyme MGAT 3, 30°C, pH 8.2
0.066
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MGAT, single enzyme 30°C, pH 8.2
0.18
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isoenzyme MGAT 2 30°C, pH 8.2
0.6
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isoenzyme MGAT 1, overall activity without separation of isoenzymes 30°C, pH 8.2
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
49000
-
x * 49000, SDS-PAGE
51000
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x * 51000, SDS-PAGE
230000 - 290000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-60°C, desalted enzyme preparation
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purification on Mono Q results in isolation of three isoenzymes, MGAT 1, MGAT 2 and MGAT 3; var. R-4218, partial
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var. R-4218, partial
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var. Topas, partial
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