Information on EC 2.6.1.73 - methionine-glyoxylate transaminase

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The expected taxonomic range for this enzyme is: Brassica

EC NUMBER
COMMENTARY hide
2.6.1.73
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RECOMMENDED NAME
GeneOntology No.
methionine-glyoxylate transaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-methionine + glyoxylate = 4-methylthio-2-oxobutanoate + glycine
show the reaction diagram
L-glutamate can also act as donor
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-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
L-methionine:glyoxylate aminotransferase
L-Glutamate can also act as donor.
CAS REGISTRY NUMBER
COMMENTARY hide
116155-75-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
var. R-4218
-
-
Manually annotated by BRENDA team
var. Topas
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-glutamine + 4-methylsulfanyl-2-oxobutanoate
2-oxoglutarate + L-methionine
show the reaction diagram
L-glutamine + glyoxylate
2-oxoglutarate + glycine
show the reaction diagram
L-histidine + glyoxylate
3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
show the reaction diagram
-
16% of activity compared to L-methionine
-
-
?
L-homoserine + glyoxylate
4-hydroxy-2-oxobutanoate + glycine
show the reaction diagram
-
56% of activity compared to L-methionine
-
-
?
L-methionine + 2-oxoglutarate
4-methylsulfanyl-2-oxobutanoate + L-glutamate
show the reaction diagram
-
19% of activity compared to glyoxylate
-
-
?
L-methionine + 4-methylsulfanyl-2-oxobutanoate
4-methylsulfanyl-2-oxobutanoate + L-methionine
show the reaction diagram
L-methionine + glyoxylate
4-methylsulfanyl-2-oxobutanoate + glycine
show the reaction diagram
L-methionine + oxaloacetate
4-methylsulfanyl-2-oxobutanoate + L-aspartate
show the reaction diagram
L-methionine + pyruvate
4-methylsulfanyl-2-oxobutanoate + L-alanine
show the reaction diagram
-
35% of activity compared to glyoxylate
-
-
?
L-methionine sulfone + glyoxylate
4-methylsulfonyl-2-oxobutanoate + glycine
show the reaction diagram
-
29% of activity compared to L-methionine
-
-
?
L-methionine sulfoxide + glyoxylate
4-methylsulfinyl-2-oxobutanoate + glycine
show the reaction diagram
-
54% of activity compared to L-methionine
-
-
?
L-methionine sulfoximine + glyoxylate
(S-methylsulfonimidoyl)-2-oxobutanoate + glycine
show the reaction diagram
-
35% of activity compared to L-methionine
-
-
?
S-methyl-L-cysteine + glyoxylate
3-methylsulfanyl-2-oxopropanoate + glycine
show the reaction diagram
-
49% of activity compared to L-methionine
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.06
-
isoenzyme MGAT 3, 30C, pH 8.2
0.066
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MGAT, single enzyme 30C, pH 8.2
0.18
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isoenzyme MGAT 2 30C, pH 8.2
0.6
-
isoenzyme MGAT 1, overall activity without separation of isoenzymes 30C, pH 8.2
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
49000
-
x * 49000, SDS-PAGE
51000
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x * 51000, SDS-PAGE
230000 - 290000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-60C, desalted enzyme preparation
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purification on Mono Q results in isolation of three isoenzymes, MGAT 1, MGAT 2 and MGAT 3; var. R-4218, partial
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var. R-4218, partial
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var. Topas, partial
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