Information on EC 2.6.1.67 - 2-aminohexanoate transaminase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.6.1.67
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RECOMMENDED NAME
GeneOntology No.
2-aminohexanoate transaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-2-aminohexanoate + 2-oxoglutarate = 2-oxohexanoate + L-glutamate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
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SYSTEMATIC NAME
IUBMB Comments
L-2-aminohexanoate:2-oxoglutarate aminotransferase
A pyridoxal-phosphate protein. Also acts on L-leucine and, more slowly, on L-isoleucine, L-2-aminopentanoate and L-aspartate.
CAS REGISTRY NUMBER
COMMENTARY hide
111310-35-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Candida guilliermondii var. membranaefaciens
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-N-methylornithine + 2-oxoglutarate
5-(methylamino)-2-oxopentanoic acid + L-glutamate
show the reaction diagram
6-N-acetyllysine + 2-oxoglutarate
6-(acetylamino)-2-oxohexanoic acid + L-glutamate
show the reaction diagram
L-aspartate + 2-oxoglutarate
oxaloacetate + L-glutamate
show the reaction diagram
L-isoleucine + 2-oxoglutarate
3-methyl-2-oxopentanoate + L-glutamate
show the reaction diagram
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + L-glutamate
show the reaction diagram
L-norleucine + 2-oxoglutarate
2-oxohexanoate + L-glutamate
show the reaction diagram
L-norvaline + 2-oxoglutarate
2-oxopentanoate + L-glutamate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + L-glutamate
show the reaction diagram
L-norleucine + 2-oxoglutarate
2-oxohexanoate + L-glutamate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
Candida guilliermondii var. membranaefaciens
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absolute requirement, probably 1 mol pyridoxal 5'-phosphate binds to each subunit, apoenzyme is inactive and regains more than 92% of original enzymatic activity after addition of pyridoxal 5'-phosphate
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
L-leucine
Candida guilliermondii var. membranaefaciens
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in presence of L-norleucine: ping-pong bi-bi inhibition with alternative substrates
L-norvaline
Candida guilliermondii var. membranaefaciens
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non-competitive inhibitor
L-valine
Candida guilliermondii var. membranaefaciens
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competitive inhibitor
additional information
Candida guilliermondii var. membranaefaciens
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6-aminohexanoic acid, L-isoleucine, L-ornithine, L-lysine are not inhibitors
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
L-lysine
Candida guilliermondii var. membranaefaciens
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this enzyme is strongly induced in vivo by growing Candida on L-lysine
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2
2-oxoglutarate
Candida guilliermondii var. membranaefaciens
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pH 7.8, 35C, in the presence of L-norleucine or L-leucine
6.6
L-leucine
Candida guilliermondii var. membranaefaciens
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in the presence of 2-oxoglutarate
1.8
L-norleucine
Candida guilliermondii var. membranaefaciens
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pH 7.8, 35C, in the presence of 2-oxoglutarate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26
Candida guilliermondii var. membranaefaciens
-
pH 7.8, 35C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8
Candida guilliermondii var. membranaefaciens
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
Candida guilliermondii var. membranaefaciens
-
assay at
55
Candida guilliermondii var. membranaefaciens
-
-
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 55
Candida guilliermondii var. membranaefaciens
-
reaction velocity increases linearly with increasing temperature at pH 7.8, above 55C activity rapidly decreases, 2-oxoglutarate and to a lesser extent pyridoxal 5'-phosphate are effective protecting agents toward heating
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
Candida guilliermondii var. membranaefaciens
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
Candida guilliermondii var. membranaefaciens
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2 * 51000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
Candida guilliermondii var. membranaefaciens
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5 min, about 20% activity remaining activity, with addition of pyridoxal 5'-phosphate 80% remaining activity, with addition of 2-oxoglutarate 100% remaining activity
60
Candida guilliermondii var. membranaefaciens
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3 min, about 17% remaining activity, with addition of pyridoxal 5'-phosphate 70% remaining activity, with addition of 2-oxoglutarate 95% remaining activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-25C, stable in the presence of 0.01 mM pyridoxal 5'-phosphate and 0.01 mM 2-oxoglutarate
Candida guilliermondii var. membranaefaciens
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate partial purification and several chromatographic steps, all steps at 4C
Candida guilliermondii var. membranaefaciens
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