Information on EC 2.6.1.40 - (R)-3-amino-2-methylpropionate-pyruvate transaminase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.6.1.40
-
RECOMMENDED NAME
GeneOntology No.
(R)-3-amino-2-methylpropionate-pyruvate transaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(R)-3-amino-2-methylpropanoate + pyruvate = 2-methyl-3-oxopropanoate + L-alanine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Valine, leucine and isoleucine degradation
-
-
pyrimidine metabolism
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-
SYSTEMATIC NAME
IUBMB Comments
(R)-3-amino-2-methylpropanoate:pyruvate aminotransferase
The two enantiomers of the 2-methyl-3-oxopropanoate formed by the enzyme interconvert by enolization, so that this enzyme, together with EC 2.6.1.22, (S)-3-amino-2-methylpropionate transaminase, provide a route for interconversion of the enantiomers of 3-amino-2-methylpropanoate.
CAS REGISTRY NUMBER
COMMENTARY hide
37279-00-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-3-amino-2-methylpropanoate + 2-oxo-n-butanoate
2-methyl-3-oxopropanoate + 2-amino-n-butanoate
show the reaction diagram
-
-
-
-
?
(R)-3-amino-2-methylpropanoate + 2-oxo-n-pentanoate
2-methyl-3-oxopropanoate + norvaline
show the reaction diagram
-
-
-
-
?
(R)-3-amino-2-methylpropanoate + glyoxylate
2-methyl-3-oxopropanoate + glycine
show the reaction diagram
(R)-3-amino-2-methylpropanoate + oxaloacetate
2-methyl-3-oxopropanoate + L-aspartate
show the reaction diagram
(R)-3-amino-2-methylpropanoate + pyruvate
2-methyl-3-oxopropanoate + L-alanine
show the reaction diagram
(S)-2-aminobutyrate + pyruvate
2-oxobutanoate + L-alanine
show the reaction diagram
-
-
-
-
?
4-aminobutanoate + pyruvate
succinic semialdehyde + L-alanine
show the reaction diagram
-
transamination at 19% the rate of (R)-3-amino-2-methylpropanoate
-
-
?
5-aminolevulinic acid + pyruvate
5-oxolevulinic acid + L-alanine
show the reaction diagram
-
-
-
-
?
arginine + pyruvate
2-oxo-5-guanidinovaleric acid + L-alanine
show the reaction diagram
-
transamination at 40% the rate of (R)-3-amino-2-methylpropanoate
-
-
?
beta-alanine + pyruvate
malonic semialdehyde + L-alanine
show the reaction diagram
DL-3-amino-n-butanoate + pyruvate
3-oxobutanoate + L-alanine
show the reaction diagram
-
-
-
-
?
DL-alpha-ethyl-beta-alanine + pyruvate
2-ethyl-3-oxopropanoate + L-alanine
show the reaction diagram
-
transamination at 33% the rate of (R)-3-amino-2-methylpropanoate
-
-
?
glycine + pyruvate
glyoxylate + L-alanine
show the reaction diagram
-
-
-
-
?
NG,NG'-dimethyl-(S)-arginine + pyruvate
? + L-alanine
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(R)-3-amino-2-methylpropanoate + pyruvate
2-methyl-3-oxopropanoate + L-alanine
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(R)-3-Amino-2-methylpropanoate
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beta-alanine as substrate
(S)-2-aminobutyrate
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beta-alanine as substrate
(S)-alanine
-
with beta-alanine + glyoxylate as substrate, not (R)-isomer
5-aminolevulinic acid
-
beta-alanine as substrate
5-Bromouracil
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1 M
5-Chlorouracil
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0.1 mM
5-Diazouracil
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1 M
5-fluorouracil
5-fluorouridine
-
weak
5-Iodouracil
-
1 M
5-Nitrouracil
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0.1 M
5-Thiouracil
-
1 M, not 2-thiouracil
6-Azathymine
-
weak
6-Azauracil
6-azauridine
-
weak
alpha-fluoro-beta-alanine
Amino-oxyacetate
-
strong
beta-Alanine
-
with (S)-alanine + glyoxylate as substrate
D-cycloserine
-
-
gabaculine
glyoxylate
-
at high concentrations
hydroxylamine
-
-
Isoorotic acid
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1 M
L-cysteine
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-
NG,NG'-dimethyl-(S)-arginine
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beta-alanine as substrate
pyruvate
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
DTT
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activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.81
beta-Alanine
0.45
pyruvate
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pH 8.8, 37C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
gamma-aminobutanoate is neither substrate nor inhibitor
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.093
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pH 8.8, 37C
1.14
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pH 8.8, 37C
11.3
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pH 8.8, 37C
additional information
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assay principle
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
pI: 6.7
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 10.6
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about half-maximal activity at pH 7.5 and pH 10.6
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
distributed throughout the brain, with high expression in the retrosplenial cortex and in hippocampal neurons
Manually annotated by BRENDA team
distributed throughout the brain, with high expression in the retrosplenial cortex and in hippocampal neurons
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000
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4 * 50000, reductive SDS-PAGE
52000
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4 * 52000, SDS-PAGE
180000
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gel filtration
220000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, at least 1 month
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-20C, concentrated enzyme solution, in 5% polyethylene glycol plus 1 mg/ml pyridoxal 5'-phosphate, several months
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4C, 0.05 mg protein/ml, in 0.1 M potassium phosphate buffer, pH 7, 1 mM EDTA, 2 mM 2-mercaptoethanol, about 50% loss of activity within a few days, 80% loss within 1 week
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4C, concentrated enzyme solution, several days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE