Information on EC 2.6.1.38 - histidine transaminase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.6.1.38
-
RECOMMENDED NAME
GeneOntology No.
histidine transaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-histidine + 2-oxoglutarate = (imidazol-5-yl)pyruvate + L-glutamate
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Histidine metabolism
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-
imidazole-lactate degradation
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-
L-histidine degradation IV
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-
histidine metabolism
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-
SYSTEMATIC NAME
IUBMB Comments
L-histidine:2-oxoglutarate aminotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
37277-92-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
N.C.I.B. 10808
-
-
Manually annotated by BRENDA team
N.C.I.B. 10808
-
-
Manually annotated by BRENDA team
mouse, inbred strains C57BL, DBA, Peru, SM and SWR
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-
Manually annotated by BRENDA team
Tue901/AEC6, mutant hut-11
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
histidine + 2-oxocaproate
imidazol-5-yl-pyruvate + L-norleucine
show the reaction diagram
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-
-
-
r
histidine + 2-oxoglutarate
imidazol-5-yl-pyruvate + L-glutamate
show the reaction diagram
-
-
-
-
r
histidine + 2-oxoisovalerate
imidazol-5-yl-pyruvate + L-valine
show the reaction diagram
-
-
-
-
r
histidine + 2-oxovalerate
imidazol-5-yl-pyruvate + L-norvaline
show the reaction diagram
-
-
-
-
r
imidazol-5-yl-pyruvate + L-glutamate
histidine + 2-oxoglutarate
show the reaction diagram
imidazol-5-yl-pyruvate + L-glutamate
L-histidine + 2-oxoglutarate
show the reaction diagram
L-histidine + 2-oxobutyrate
imidazol-5-yl-pyruvate + 2-aminobutanoate
show the reaction diagram
-
-
-
-
r
L-histidine + glyoxylate
imidazol-5-yl-pyruvate + glycine
show the reaction diagram
-
-
-
-
r
L-histidine + pyruvate
imidazol-5-yl-pyruvate + L-alanine
show the reaction diagram
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ping pong reaction mechanism
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-
r
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
imidazol-5-yl-pyruvate + L-glutamate
histidine + 2-oxoglutarate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Bromopyruvate
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-
p-chloromercuribenzoate
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-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.5
imidazolylpyruvate
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pH 8.0, 30°C
5
L-glutamate
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pH 8.0, 30°C
4 - 25
L-histidine
10
pyruvate
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pH 8.0, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0015
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substrate L-tryptophan
0.0019
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substrate L-histidine
0.002
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substrate L-tyrosine
0.0021
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substrate L-phenylalanine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8.5
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optimal activity in this pH range, outside this range activity decreases sharply
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45000
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2 * 45000, SDS-PAGE
70000
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gel filtration
85000
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gel filtration
110000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
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2 * 45000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50 - 70
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preincubation of the reaction mixture at 50, 60 and 70°C results in a increase of the reaction rate
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
purified preparations are unstable, activity is lost during purification, EDTA, DTT or 2-mercaptoethanol stabilizes during purification, pyridoxal 5'-phosphate restores activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE