Information on EC 2.6.1.31 - pyridoxamine-oxaloacetate transaminase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.6.1.31
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RECOMMENDED NAME
GeneOntology No.
pyridoxamine-oxaloacetate transaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
pyridoxamine + oxaloacetate = pyridoxal + L-aspartate
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Vitamin B6 metabolism
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-
Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
pyridoxamine:oxaloacetate aminotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
37277-88-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
pyridoxamine + oxaloacetate
pyridoxal + L-aspartate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
pyridoxamine + oxaloacetate
pyridoxal + L-aspartate
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-oxoglutarate
-
inhibition at pH 7.0 is higher than at pH 8.5, 3.33 mM in presence of equimolar concentrations of oxaloacetate
2-Oxomalonate
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inhibition at pH 7.0 is higher than at pH 8.5, 0.4 mM in presence of equimolar concentrations of oxaloacetate
3,3-Dimethylglutamic acid
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not at pH 7.0, weak at pH 8.0
3-oxoadipate
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inhibition at pH 7.0 is higher than at pH 8.5, 3.33 mM in presence of equimolar concentrations of oxaloacetate
adipic acid
-
not at pH 7.0, weak at pH 8.0
alpha-glycerophosphate
Benzylmalonic acid
-
not at pH 7.0, weak at pH 8.0
beta-Glycerophosphate
Diethylstilbestrol diphosphate
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-
L-aspartate
L-glutamate
pyridoxal 5'-phosphate
pyridoxamine phosphate
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
arsenate
-
activation, less effective than phosphate
D-Aspartate
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increases activity
diphosphate
-
activation, less effective than phosphate
phosphate
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essential, activation in absence of diethylstilbestrol and pyridoxal 5'-phosphate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.012 - 0.57
oxaloacetate
0.7 - 2.3
pyridoxamine
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.066
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purified enzyme
0.076
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partially purified enzyme
0.081
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partially purified enzyme
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 9.5
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about 50% of activity maximum at pH 7.5 and pH 9.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
frozen, 20% loss of activity after 2 months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE