Information on EC 2.6.1.26 - thyroid-hormone transaminase

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The expected taxonomic range for this enzyme is: Oryctolagus cuniculus

EC NUMBER
COMMENTARY hide
2.6.1.26
-
RECOMMENDED NAME
GeneOntology No.
thyroid-hormone transaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-3,5,3'-triiodothyronine + 2-oxoglutarate = 3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
L-3,5,3'-triiodothyronine:2-oxoglutarate aminotransferase
A pyridoxal-phosphate protein. Acts on monoiodotyrosine, diiodotyrosine, triiodothyronine, thyroxine and dinitrotyrosine (unlike EC 2.6.1.24 diiodotyrosine transaminase, which does not act on dinitrotyrosine). Pyruvate or oxaloacetate can act as acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
51004-29-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,5-dibromo-L-tyrosine + 2-oxoglutarate
3-(3,5-dibromo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
show the reaction diagram
-
-
-
-
r
3,5-diiodo-D-tyrosine + 2-oxoglutarate
3-(3,5-diiodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
show the reaction diagram
-
very low activity
-
-
r
3,5-diiodo-L-tyrosine + 2-oxoglutarate
3-(3,5-diiodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
show the reaction diagram
-
-
-
-
r
3,5-dinitro-L-tyrosine + 2-oxoglutarate
3-(3,5-dinitro-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
show the reaction diagram
-
-
i.e. 3,5-dinitro-p-hydroxyphenylpyruvic acid + Glu
r
3-amino-L-tyrosine + 2-oxoglutarate
3-(3-amino-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
show the reaction diagram
-
very low activity
-
-
r
3-iodo-L-tyrosine + 2-oxoglutarate
3-(3-iodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
show the reaction diagram
-
-
-
-
r
3-nitro-L-tyrosine + 2-oxoglutarate
3-(3-nitro-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
show the reaction diagram
-
-
-
-
r
L-3,5,3'-triiodothyronine + 2-oxoglutarate
3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate
show the reaction diagram
-
-
i.e. 3,5,3'-triiodothyropyruvate + L-Glu
r
L-3,5,3'-triiodothyronine + oxaloacetate
3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + glycine
show the reaction diagram
-
-
i.e. 3,5,3'-triiodothyrophenylpyruvate + Gly
r
L-3,5,3'-triiodothyronine + pyruvate
3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-alanine
show the reaction diagram
-
-
i.e. 3,5,3'-triiodothyrophenylpyruvate + L-Ala
r
L-phenylalanine + 2-oxoglutarate
3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
show the reaction diagram
-
low activtiy
-
-
r
L-thyroxine + 2-oxoglutarate
3-[4-(4-hydroxy-3,5-diiodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate
show the reaction diagram
-
-
-
-
r
L-tryptophan + 2-oxoglutarate
3-indole-2-oxopropanoate + L-glutamate
show the reaction diagram
-
low activtiy
-
-
r
L-tyrosine + 2-oxoglutarate
2-oxo-3-phenylpropanoate + L-glutamate
show the reaction diagram
-
low activtiy
-
-
r
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-3,5,3'-triiodothyronine + 2-oxoglutarate
3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate
show the reaction diagram
-
-
i.e. 3,5,3'-triiodothyropyruvate + L-Glu
r
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
partial requirement
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
p-hydroxymercuribenzoate
-
complete inhibition at 0.1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
absolutely required
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2 - 2
2-oxoglutarate
0.9
3,5-diiodo-L-tyrosine
-
pH 7.8, 37C
4.4
3,5-Dinitro-L-tyrosine
-
pH 7.8, 37C
3.8
3-iodo-L-tyrosine
-
pH 7.8, 37C
2.12
L-thyronine
-
pH 7.8, 37C
0.034
L-triiodothyronine
-
pH 7.8, 37C
2
pyruvate
-
cosubstrate L-triiodothyronine, pH 7.8, 37C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.1 - 6.6
3,5-diiodo-L-tyrosine
39.9
3,5-Dinitro-L-tyrosine
Oryctolagus cuniculus
-
pH 7.8, 37C
3.68
3-iodo-L-tyrosine
Oryctolagus cuniculus
-
pH 7.8, 37C
0.0233
L-thyroxine
Oryctolagus cuniculus
-
pH 7.8, 37C
0.0317
L-triiodothyronine
Oryctolagus cuniculus
-
pH 7.8, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.013
-
purified enzyme, substrates L-3,5,3'-triiodothyronine + 2-oxoglutarate
15
-
purified enzyme, substrates 3,5-dinitro-L-tyrosine + 2-oxoglutarate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4 - 8.6
-
broad optimum
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8.6
-
pH 7.0: 30% of maximal activity with L-triiodothyronine, 15% of maximal activity with 3,5-dinitro-L-tyrosine, pH 7.4-8.6: maximal activity with triiodothyronine and 3,5-dinitro-L-tyrosine
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
low activity
Manually annotated by BRENDA team
-
low activity
Manually annotated by BRENDA team
-
low activity
Manually annotated by BRENDA team
additional information
-
not in serum
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48000
-
2 * 48000, SDS-PAGE
95000
-
glycerol density gradient centrifugation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 48000, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 30% loss of activity per month
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE