Information on EC 2.6.1.22 - (S)-3-amino-2-methylpropionate transaminase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.6.1.22
-
RECOMMENDED NAME
GeneOntology No.
(S)-3-amino-2-methylpropionate transaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(S)-3-amino-2-methylpropanoate + 2-oxoglutarate = 2-methyl-3-oxopropanoate + L-glutamate
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-valine degradation I
-
-
Valine, leucine and isoleucine degradation
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-
SYSTEMATIC NAME
IUBMB Comments
(S)-3-amino-2-methylpropanoate:2-oxoglutarate aminotransferase
Also acts on beta-alanine and other omega-amino acids having carbon chains between 2 and 5. The two enantiomers of the 2-methyl-3-oxopropanoate formed by the enzyme interconvert by enolization, so that this enzyme, together with EC 2.6.1.40, (R)-3-amino-2-methylpropionate---pyruvate transaminase, provide a route for interconversion of the enantiomers of 3-amino-2-methylpropanoate.
CAS REGISTRY NUMBER
COMMENTARY hide
9031-95-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-3-amino-2-methylpropanoate + 2-oxoglutarate
2-methyl-3-oxopropanoate + L-glutamate
show the reaction diagram
(S)-3-amino-2-methylpropanoate + pyruvate
D-methylmalonate semialdehyde + L-alanine
show the reaction diagram
-
-
-
r
4-aminobutanoate + 2-oxoglutarate
succinic semialdehyde + L-glutamate
show the reaction diagram
5-aminopentanoate + 2-oxoglutarate
5-oxopentanoate + L-glutamate
show the reaction diagram
beta-alanine + 2-oxoglutarate
malonic semialdehyde + L-glutamate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-3-amino-2-methylpropanoate + 2-oxoglutarate
2-methyl-3-oxopropanoate + L-glutamate
show the reaction diagram
-
involved in omega-amino acid metabolism in mammals
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-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(S)-3-amino-2-methylpropanoate
-
beta-alanine as substrate, not (R)-isomer
2-oxoglutarate
-
in excess, substrate inhibition
5-fluorouracil
-
-
5-Iodouracil
-
-
6-Azathymine
-
-
6-Azauracil
-
-
Antiserum against beta-alanine:2-oxoglutarate aminotransferase
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-
-
gabaculine
-
-
pyridoxal 5'-phosphate
-
inhibits at high concentrations, activates at low concentrations
additional information
-
not inhibitory: 6-azauridine, 6-azauridine 5-phosphate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.7
(S)-3-amino-2-methylpropanoate
-
pH 8.8, 37C
1.1
beta-Alanine
-
pH 8.8, 37C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.7
(S)-3-amino-2-methylpropanoate
-
pH 8.8, 37C
1.8 - 1.9
5-fluorouracil
0.7
6-Azauracil
-
pH 8.8, 37C
0.0071
gabaculine
-
pH 8.8, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.047
-
kidney enzyme, pH 8.8, 37C
0.096
-
brain enzyme, pH 8.8, 37C
0.133
-
liver enzyme, pH 8.8, 37C
0.185
-
pH 7.5, 37C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.1
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5 - 9.5
-
about half-maximal activity at pH 8.5 and 9.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
105000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 x 56000, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, in 0.05 M phosphate buffer, pH 7.5, at least 3 weeks
-
-25C, in 0.1 M phosphate buffer, pH 7.0, 1mM EDTA, 2 mM 2-mercaptoethanol, 0.04 mM pyridoxal-5'-phosphate, few days, 50% loss of activity
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4C, in 0.1 M phosphate buffer, pH 7.0, 1mM EDTA, 2 mM 2-mercaptoethanol, 0.04 mM pyridoxal-5'-phosphate, 1 week, no loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Show AA Sequence (205 entries)
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