Information on EC 2.5.1.B35 - tetratrans,hexacis-undecaprenyl-diphosphate synthase [geranylfarnesyl-diphosphate specific]

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The expected taxonomic range for this enzyme is: Aeropyrum pernix

EC NUMBER
COMMENTARY hide
2.5.1.B35
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
tetratrans,hexacis-undecaprenyl-diphosphate synthase [geranylfarnesyl-diphosphate specific]
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
geranylfarnesyl diphosphate + 6 isopentenyl diphosphate = 6 diphosphate + tetratrans,hexacis-undecaprenyl diphosphate
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
geranylfarnesyl diphosphate:isopentenyl-diphosphate cistransferase (adding 6 isopentenyl units)
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
show the reaction diagram
about 95% of the activity with geranylfarnesyl diphosphate. The main product is undecaprenyl diphosphate, regardless of the substrate. If the ratio of IPP to the allylic substrate becomes lower, the product will be shorter, probably because of the shortage of isopentenyl diphosphate or because of the frequent dissociation of an intermediate from the active site by competitive binding of the hydrophobic allylic substrate
the stereochemistry of the product is not experimentally determined
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?
geranyl diphosphate + 9 isopentenyl diphosphate
9 diphosphate + monotrans,nonacis-undecaprenyl diphosphate
show the reaction diagram
about 65% of the activity with geranylfarnesyl diphosphate. The main product is undecaprenyl diphosphate, regardless of the substrate. If the ratio of IPP to the allylic substrate becomes lower, the product will be shorter, probably because of the shortage of isopentenyl diphosphate or because of the frequent dissociation of an intermediate from the active site by competitive binding of the hydrophobic allylic substrate
the stereochemistry of the product is not experimentally determined
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?
geranylfarnesyl diphosphate + 6 isopentenyl diphosphate
6 diphosphate + tetratrans,hexacis-undecaprenyl diphosphate
show the reaction diagram
geranylgeranyl diphosphate + 7 isopentenyl diphosphate
7 diphosphate + tritrans,heptacis-undecaprenyl diphosphate
show the reaction diagram
about 45% of the activity with geranylfarnesyl diphosphate. The main product is undecaprenyl diphosphate, regardless of the substrate. If the ratio of IPP to the allylic substrate becomes lower, the product will be shorter, probably because of the shortage of isopentenyl diphosphate or because of the frequent dissociation of an intermediate from the active site by competitive binding of the hydrophobic allylic substrate. When the ratio of isopentenyl diphosphate to geranylfarnesyl diphosphate is decreased to 1, the UPP synthase predominately yields shorter C30–45 products
the stereochemistry of the product is not experimentally determined
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?
additional information
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dimethylallyl diphosphate does not react with the enzyme. If the ratio of IPP to the allylic substrate becomes lower, the product will be shorter, probably because of the shortage of isopentenyl diphosphate or because of the frequent dissociation of an intermediate from the active site by competitive binding of the hydrophobic allylic substrate
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
geranylfarnesyl diphosphate + 6 isopentenyl diphosphate
6 diphosphate + tetratrans,hexacis-undecaprenyl diphosphate
show the reaction diagram
Q9YC66
the enzyme is involved in the biosynthetic pathway for isoprenoid compounds
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
a divalent metal ion is necessary for the activity. Ca2+, Mn2+ and Cu2+ all confer significantly lower activity than Mg2+ does
Cu2+
a divalent metal ion is necessary for the activity. Ca2+, Mn2+ and Cu2+ all confer significantly lower activity than Mg2+ does
Mg2+
a divalent metal ion is necessary for the activity. The addition of 5 mM Mg2+ gives the highest activity within the conditions tested. A 10fold higher concentration of Mg2+ continues to show activity that is comparable to 5 mM Mg2+
Mn2+
a divalent metal ion is necessary for the activity. Ca2+, Mn2+ and Cu2+ all confer significantly lower activity than Mg2+ does
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
5 mM, complete inhibition
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
90
1 h, the enzyme retains more than 80% of its activity
100
1 h, complete inactivation
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli