Information on EC 2.5.1.B31 - 5-dimethylallyltryptophan synthase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.5.1.B31
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
5-dimethylallyltryptophan synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dimethylallyl diphosphate + L-tryptophan = diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Friedel-Crafts alkylation
SYSTEMATIC NAME
IUBMB Comments
dimethylallyl-diphosphate:L-tryptophan 5-dimethylallyltransferase
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
gene SCO7467
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
metabolism
-
enzyme 5-DMATSSc enzyme is involved in biosynthesis of dimethylallylindole 3-acetonitrile
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-pentenyl diphosphate + L-tryptophan
diphosphate + 5-(pent-2-enyl)-L-tryptophan
show the reaction diagram
2-pentenyl diphosphate + L-tryptophan
diphosphate + 6-(2-trans-pentenyl)-L-tryptophan
show the reaction diagram
-
91% of the activity with dimethylallyl diphosphate
-
-
?
2-pentenyl diphosphate + L-tryptophan
diphosphate + 6-(pent-2-enyl)-L-tryptophan
show the reaction diagram
2-pentenyl diphosphate + L-tryptophan
diphosphate + 7-(pent-2-enyl)-L-tryptophan
show the reaction diagram
benzyl diphosphate + L-tryptophan
diphosphate + 5-benzyl-L-tryptophan
show the reaction diagram
-
C-5 prenylation
-
-
?
benzyl diphosphate + L-tryptophan
diphosphate + 6-benzyl-L-tryptophan
show the reaction diagram
dimethylallyl diphosphate + 1-methyl-DL-tryptophan
diphosphate + 1-methyl-5-(3-methylbut-2-en-1-yl)-tryptophan
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + 1-methyl-L-tryptophan
diphosphate + 1-methyl-5-(3-methylbut-2-enyl)-L-tryptophan
show the reaction diagram
-
46.9% of the activity with L-Trp
-
-
?
dimethylallyl diphosphate + 4-methyl-DL-tryptophan
diphosphate + 4-methyl-5-(3-methylbut-2-en-1-yl)-tryptophan
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + 4-methyl-L-tryptophan
diphosphate + 4-methyl-5-(3-methylbut-2-enyl)-L-tryptophan
show the reaction diagram
-
56.6% of the activity with L-Trp
-
-
?
dimethylallyl diphosphate + 5-methyl-DL-tryptophan
diphosphate + 5-methyl-5-(3-methylbut-2-enyl)-DL-tryptophan
show the reaction diagram
-
8.1% of the activity with L-Trp
-
-
?
dimethylallyl diphosphate + 6-benzyl-L-tryptophan
diphosphate + 5-benzyl-L-tryptophan
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + 6-benzyl-L-tryptophan
diphosphate + 7-benzyl-L-tryptophan
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + 6-benzyl-L-tryptophan
diphosphate + 7-benzyl-L-tryptophan + 5-benzyl-L-tryptophan
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + 6-fluoro-DL-tryptophan
diphosphate + 6-fluoro-5-(3-methylbut-2-en-1-yl)tryptophan
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + 6-fluoro-L-tryptophan
diphosphate + 6-fluoro-5-(3-methylbut-2-enyl)-L-tryptophan
show the reaction diagram
-
69.5% of the activity with L-Trp
-
-
?
dimethylallyl diphosphate + 6-methyl-DL-tryptophan
diphosphate + 6-methyl-5-(3-methylbut-2-en-1-yl)-tryptophan
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + 6-methyl-L-tryptophan
diphosphate + 6-methyl-5-(3-methylbut-2-enyl)-L-tryptophan
show the reaction diagram
-
53.2% of the activity with L-Trp
-
-
?
dimethylallyl diphosphate + 6-[(2E)-but-2-en-1-yl]-L-tryptophan
diphosphate + 5-[(2E)-but-2-en-1-yl]-L-tryptophan
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + 6-[(2E)-but-2-en-1-yl]-L-tryptophan
diphosphate + 7-[(2E)-but-2-en-1-yl]-L-tryptophan + 5-[(2E)-but-2-en-1-yl]-L-tryptophan
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + 6-[(2E)-pent-2-en-1-yl]-L-tryptophan
diphosphate + 7-[(2E)-pent-2-en-1-yl]-L-tryptophan + 5-[(2E)-pent-2-en-1-yl]-L-tryptophan
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + 7-methyl-DL-tryptophan
diphosphate + 7-methyl-5-(3-methylbut-2-en-1-yl)-tryptophan
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + 7-methyl-DL-tryptophan
diphosphate + 7-methyl-5-(3-methylbut-2-enyl)-DL-tryptophan
show the reaction diagram
-
57.8% of the activity with L-Trp
-
-
?
dimethylallyl diphosphate + ardeemin fumiquinazoline
diphosphate + (1S,4R)-4-methyl-1-[[5-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione + (1S,4R)-4-methyl-1-[[6-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + ardeemin fumiquinazoline
diphosphate + (1S,4R)-4-methyl-1-[[5-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione + (1S,4R)-4-methyl-1-[[6-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione + (1S,4R)-4-methyl-1-[[7-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione + (1S,4R)-1-[[5,6-bis(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-4-methyl-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + dia,ent-ardeemin fumiquinazoline
diphosphate + (1R,4R)-4-methyl-1-[[5-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione + (1R,4R)-4-methyl-1-[[6-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + dia,ent-ardeemin fumiquinazoline
diphosphate + (1R,4R)-4-methyl-1-[[6-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + dia-ardeemin fumiquinazoline
diphosphate + (1S,4S)-4-methyl-1-[[5-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione + (1S,4S)-4-methyl-1-[[6-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + dia-ardeemin fumiquinazoline
diphosphate + (1S,4S)-4-methyl-1-[[6-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + DL-indole-3-lactate
diphosphate + 2-hydroxy-3-[5-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]propanoic acid
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + DL-indole-3-lactic acid
?
show the reaction diagram
-
67.3% of the activity with L-Trp
-
-
?
dimethylallyl diphosphate + ent-ardeemin fumiquinazoline
diphosphate + (1R,4S)-4-methyl-1-[[5-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione + (1R,4S)-4-methyl-1-[[6-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + ent-ardeemin fumiquinazoline
diphosphate + (1R,4S)-4-methyl-1-[[5-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione + (1R,4S)-4-methyl-1-[[6-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione + (1R,4S)-4-methyl-1-[[7-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione + (1R,4S)-1-[[5,6-bis(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-4-methyl-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + indole-3-propionate
diphosphate + 3-[5-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]propanoic acid
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + indole-3-propionic acid
?
show the reaction diagram
-
70.7% of the activity with L-Trp
-
-
?
dimethylallyl diphosphate + L-abrine
?
show the reaction diagram
-
90.9% of the activity with L-Trp
-
-
?
dimethylallyl diphosphate + L-abrine
diphosphate + N-methyl-5-(3-methylbut-2-en-1-yl)-L-tryptophan
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + L-beta-homo-L-tryptophan
?
show the reaction diagram
-
48.8% of the activity with L-Trp
-
-
?
dimethylallyl diphosphate + L-beta-homotryptophan
diphosphate + (3S)-3-amino-4-[5-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]butanoic acid
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + L-o-Tyr
diphosphate + 3-(3-methylbut-2-enyl)-L-o-Tyr
show the reaction diagram
-
C-3 prenylation
3-dimethylallyl-L-tyrosine
-
?
dimethylallyl diphosphate + L-o-tyrosine
diphosphate + 5-(3-methylbut-2-enyl)-L-o-tyrosine
show the reaction diagram
-
C-5 prenylation, proposed reaction mechanism, overview. Low activity
-
-
?
dimethylallyl diphosphate + L-tryptophan
diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan
show the reaction diagram
dimethylallyl diphosphate + L-tyrosine
diphosphate + 3-(3-methylbut-2-enyl)-L-tyrosine
show the reaction diagram
dimethylallyl diphosphate + N-acetyl-DL-tryptophan
diphosphate + N-acetyl-5-(3-methylbut-2-en-1-yl)tryptophan
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + N-acetyl-DL-tryptophan
diphosphate + N-acetyl-5-(3-methylbut-2-enyl)-DL-tryptophan
show the reaction diagram
-
54.4% of the activity with L-Trp
-
-
?
dimethylallyl diphosphate + trans-indole-3-acrylate
diphosphate + (2E)-3-[5-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]prop-2-enoic acid
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + trans-indole-3-acrylic acid
?
show the reaction diagram
-
38.0% of the activity with L-Trp
-
-
?
methylallyl diphosphate + L-tryptophan
diphosphate + 5-(2-trans-butenyl)-L-tryptophan + 6-(2-trans-butenyl)-L-tryptophan
show the reaction diagram
-
22% of the activity with dimethylallyl diphosphate
-
-
?
methylallyl diphosphate + L-tryptophan
diphosphate + 5-(but-2-enyl)-L-tryptophan
show the reaction diagram
-
C-5 and C-6 prenylation
-
-
?
methylallyl diphosphate + L-tryptophan
diphosphate + 6-(but-2-enyl)-L-tryptophan
show the reaction diagram
-
C-5 and C-6 prenylation
-
-
?
methylallyl diphosphate + L-tryptophan
phosphate + 5-(but-2-enyl)-L-tryptophan
show the reaction diagram
methylallyl diphosphate + L-tryptophan
phosphate + 6-(but-2-enyl)-L-tryptophan
show the reaction diagram
methylallyl diphosphate + L-tryptophan
phosphate + 7-(but-2-enyl)-L-tryptophan
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dimethylallyl diphosphate + L-tryptophan
diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
activates 2fold
additional information
-
the SCO7467-catalyzed prenylation reaction is Mg2+-independent
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no inhibition by EDTA at 5 mM
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1
1-methyl-DL-tryptophan
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
0.028 - 0.131
2-pentenyl diphosphate
0.25
4-methyl-DL-tryptophan
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
0.27
6-fluoro-DL-tryptophan
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
1
6-methyl-DL-tryptophan
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
0.47
7-methyl-DL-tryptophan
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
0.21
ardeemin fumiquinazoline
-
0.04 - 0.11
benzyl diphosphate
-
0.05 - 0.076
dimethylallyl diphosphate
0.67
DL-indole-3-lactate
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
0.2
ent-ardeemin fumiquinazoline
-
0.39
indole-3-propionate
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
0.26
L-abrine
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
0.97
L-beta-homotryptophan
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
0.49
L-o-tyrosine
-
pH 7.5, 37°C, recombinant enzyme
-
0.034 - 0.08
L-tryptophan
0.04 - 0.12
methylallyl diphosphate
0.35
N-acetyl-DL-tryptophan
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
0.4
trans-indole-3-acrylate
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.059
1-methyl-DL-tryptophan
Aspergillus clavatus
A1CRX0
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
0.0014 - 0.023
2-pentenyl diphosphate
0.29
4-methyl-DL-tryptophan
Aspergillus clavatus
A1CRX0
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
0.29
6-fluoro-DL-tryptophan
Aspergillus clavatus
A1CRX0
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
0.76
6-methyl-DL-tryptophan
Aspergillus clavatus
A1CRX0
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
0.4
7-methyl-DL-tryptophan
Aspergillus clavatus
A1CRX0
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
0.027
ardeemin fumiquinazoline
-
0.00028 - 0.005
benzyl diphosphate
-
0.4 - 1.3
dimethylallyl diphosphate
0.43
DL-indole-3-lactate
Aspergillus clavatus
A1CRX0
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
0.022
ent-ardeemin fumiquinazoline
-
0.39
indole-3-propionate
Aspergillus clavatus
A1CRX0
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
0.4
L-abrine
Aspergillus clavatus
A1CRX0
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
0.15
L-beta-homotryptophan
Aspergillus clavatus
A1CRX0
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
0.013
L-o-tyrosine
Aspergillus clavatus
-
pH 7.5, 37°C, recombinant enzyme
-
0.4 - 0.87
L-tryptophan
0.00048 - 0.005
methylallyl diphosphate
0.1
N-acetyl-DL-tryptophan
Aspergillus clavatus
A1CRX0
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
0.14
trans-indole-3-acrylate
Aspergillus clavatus
A1CRX0
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.59
1-methyl-DL-tryptophan
Aspergillus clavatus
A1CRX0
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
5382
0.174
2-pentenyl diphosphate
Aspergillus clavatus
-
37°C, pH not specified in the publication
15354
1.16
4-methyl-DL-tryptophan
Aspergillus clavatus
A1CRX0
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
11621
1.074
6-fluoro-DL-tryptophan
Aspergillus clavatus
A1CRX0
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
4677
0.76
6-methyl-DL-tryptophan
Aspergillus clavatus
A1CRX0
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
4145
0.851
7-methyl-DL-tryptophan
Aspergillus clavatus
A1CRX0
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
6249
1.538
dimethylallyl diphosphate
Aspergillus clavatus
A1CRX0
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
157
0.642
DL-indole-3-lactate
Aspergillus clavatus
A1CRX0
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
42434
1
indole-3-propionate
Aspergillus clavatus
A1CRX0
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
13185
1.538
L-abrine
Aspergillus clavatus
A1CRX0
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
27257
0.155
L-beta-homotryptophan
Aspergillus clavatus
A1CRX0
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
7715
0.0265
L-o-tyrosine
Aspergillus clavatus
-
pH 7.5, 37°C, recombinant enzyme
214008
25.59
L-tryptophan
Aspergillus clavatus
A1CRX0
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
119
0.136
methylallyl diphosphate
Aspergillus clavatus
-
37°C, pH not specified in the publication
15353
0.286
N-acetyl-DL-tryptophan
Aspergillus clavatus
A1CRX0
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
9226
0.35
trans-indole-3-acrylate
Aspergillus clavatus
A1CRX0
pH 7.5, 37°C, recombinant His-tagged 5-DMATS
42435
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 37
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
purified recombinant enzyme, loss of 50% activity after 20 min, loss of over 90% activity after 60 min, inactivation after 120 min
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
recombinant enzyme from in Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
-
recombinant His-tagged 5-DMATS from Escherichia coli strain BL21 or Saccharomyces cerevisiae strain INVSc1 by nickel affinity chromatography
recombinant His8-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant solube His-tagged 5-DMATS from Escherichia coli strain M15 by nickel affinity chromatography to near homogeneity
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
5-DMATS, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strains BL21, XL1 Blue, or M15 or in Saccharomyces cerevisiae strain INVSc1
gene ACLA_031240 or 5-dmats, DNA and amino acid sequence determination and analysis, expression as solube His-tagged protein in Escherichia coli strain M15, subcloning in Escherichia coli strain XL1 Blue
gene SCO7467, recombinant expression of His8-tagged enzyme in Escherichia coli strain BL21(DE3)
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gene SCO7468, genetic organization and sequence comparison, functional recombinant expression in Streptomyces lividans strain TK23, the overexpression of 5-DMATSSc results in 5-dimethylallylindole-3-acetonitrile overproduction in the transformant. The heterologous host Streptomyces lividans TK23 possesses a gene cluster almost identical to that of Streptomyces coelicolor A3(2). Recombinant expression in Escherichia coli strain BL21(DE3)
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recombinant overexpression
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
additional information
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prenyltransferases of the dimethylallyl-tryptophan synthase superfamily catalyze Friedel-Crafts alkylation with high flexibility for aromatic substrates, but the high specificity for dimethylallyl diphosphate prohibits their application as biocatalysts