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Information on EC 2.5.1.B25 - 2,7-dihydroxynaphthalene 3-dimethylallyltransferase Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The enzyme appears in viruses and cellular organisms
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2.5.1.B25
preliminary BRENDA-supplied EC number
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2,7-dihydroxynaphthalene 3-dimethylallyltransferase
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dimethylallyl diphosphate + 2,7-dihydroxynaphthalene = diphosphate + 2,7-dihydroxy-3-dimethylallylnaphthalene
dimethylallyl diphosphate + 2,7-dihydroxynaphthalene = diphosphate + 2,7-dihydroxy-3-dimethylallylnaphthalene
Friedel-Crafts alkylation
dimethylallyl diphosphate + 2,7-dihydroxynaphthalene = diphosphate + 2,7-dihydroxy-3-dimethylallylnaphthalene
Friedel-Crafts alkylation
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dimethylallyl diphosphate + 2,7-dihydroxynaphthalene = diphosphate + 2,7-dihydroxy-3-dimethylallylnaphthalene
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dimethylallyl diphosphate:2,7-dihydroxynaphthalene 3-dimethylallyltransferase
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PtfAt
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PtfBf
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PtfSs
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UniProt
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UniProt
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dimethylallyl diphosphate + 2,7-dihydroxynaphthalene
diphosphate + 2,7-dihydroxy-3-dimethylallylnaphthalene
additional information
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dimethylallyl diphosphate + 2,7-dihydroxynaphthalene
diphosphate + 2,7-dihydroxy-3-dimethylallylnaphthalene
the enzyme is specific for dimethylallyl diphosphate. No activity with geranyl diphosphate or isopentenyl diphosphate
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?
dimethylallyl diphosphate + 2,7-dihydroxynaphthalene
diphosphate + 2,7-dihydroxy-3-dimethylallylnaphthalene
the enzyme is specific for dimethylallyl diphosphate. No activity with geranyl diphosphate or isopentenyl diphosphate
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?
dimethylallyl diphosphate + 2,7-dihydroxynaphthalene
diphosphate + 2,7-dihydroxy-3-dimethylallylnaphthalene
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the enzyme is specific for dimethylallyl diphosphate. No activity with geranyl diphosphate or isopentenyl diphosphate
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?
dimethylallyl diphosphate + 2,7-dihydroxynaphthalene
diphosphate + 2,7-dihydroxy-3-dimethylallylnaphthalene
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the enzyme is specific for dimethylallyl diphosphate. No activity with geranyl diphosphate or isopentenyl diphosphate
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-
?
dimethylallyl diphosphate + 2,7-dihydroxynaphthalene
diphosphate + 2,7-dihydroxy-3-dimethylallylnaphthalene
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the enzyme is specific for dimethylallyl diphosphate. No activity with geranyl diphosphate or isopentenyl diphosphate
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?
dimethylallyl diphosphate + 2,7-dihydroxynaphthalene
diphosphate + 2,7-dihydroxy-3-dimethylallylnaphthalene
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the enzyme is specific for dimethylallyl diphosphate. No activity with geranyl diphosphate or isopentenyl diphosphate
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additional information
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significantly lower level of prenylation activity with dihydrophenazine-1-carboxylic acid as aromatic substrate, and only traces of products are detected with aspulvinone E, flaviolin, or 4-hydroxybenzoic acid. No product is formed with L-tryptophan, L-tyrosine, or 4-hydroxyphenylpyruvate
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additional information
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significantly lower level of prenylation activity with dihydrophenazine-1-carboxylic acid as aromatic substrate, and only traces of products are detected with aspulvinone E, flaviolin, or 4-hydroxybenzoic acid. No product is formed with L-tryptophan, L-tyrosine, or 4-hydroxyphenylpyruvate
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additional information
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significantly lower level of prenylation activity with dihydrophenazine-1-carboxylic acid or 4-hydroxybenzoate as aromatic substrate, and only traces of products are detected with aspulvinone E, flaviolin. No product is formed with L-tryptophan, L-tyrosine, or 4-hydroxyphenylpyruvate
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additional information
?
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significantly lower level of prenylation activity with dihydrophenazine-1-carboxylic acid or 4-hydroxybenzoate as aromatic substrate, and only traces of products are detected with aspulvinone E, flaviolin. No product is formed with L-tryptophan, L-tyrosine, or 4-hydroxyphenylpyruvate
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additional information
?
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significantly lower level of prenylation activity with dihydrophenazine-1-carboxylic acid as aromatic substrate, and only traces of products are detected with aspulvinone E, flaviolin, or 4-hydroxybenzoic acid. No product is formed with L-tryptophan, L-tyrosine, or 4-hydroxyphenylpyruvate
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additional information
?
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significantly lower level of prenylation activity with dihydrophenazine-1-carboxylic acid as aromatic substrate, and only traces of products are detected with aspulvinone E, flaviolin, or 4-hydroxybenzoic acid. No product is formed with L-tryptophan, L-tyrosine, or 4-hydroxyphenylpyruvate
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Mg2+
addition of MgCl2 to the incubation mixture increases the reaction velocity. The optimal concentration of MgCl2 is 10 mM, leading to a 2.4-fold increase of product formation. The enzyme shows catalytic activity in the complete absence of Mg2+ or any other divalent cation
Mg2+
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the enzyme is active in the absence of Mg2+, but addition of 10 mM MgCl2 increases reaction velocity by a factor of 2.6
Mg2+
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the enzyme is active in the absence of Mg2+, but addition of 10 mM MgCl2 increases reaction velocity by a factor of 2.6
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2,7-dihydroxynaphthalene
20 mM, almost complete inhibition
2,7-dihydroxynaphthalene
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20 mM, almost complete inhibition
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0.324 - 0.996
2,7-dihydroxynaphthalene
0.325 - 1.232
dimethylallyl diphosphate
0.324
2,7-dihydroxynaphthalene
pH 8.7, 30°C
0.59
2,7-dihydroxynaphthalene
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pH 8.7, 30°C
0.996
2,7-dihydroxynaphthalene
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pH 8.7, 30°C
0.325
dimethylallyl diphosphate
pH 8.7, 30°C
1.01
dimethylallyl diphosphate
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pH 8.7, 30°C
1.232
dimethylallyl diphosphate
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pH 8.7, 30°C
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0.026
2,7-dihydroxynaphthalene
pH 8.7, 30°C
0.026
dimethylallyl diphosphate
pH 8.7, 30°C
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0.005 - 0.08
2,7-dihydroxynaphthalene
0.005 - 0.08
dimethylallyl diphosphate
0.005
2,7-dihydroxynaphthalene
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pH 8.7, 30°C
0.022
2,7-dihydroxynaphthalene
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pH 8.7, 30°C
0.08
2,7-dihydroxynaphthalene
pH 8.7, 30°C
0.005
dimethylallyl diphosphate
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pH 8.7, 30°C
0.022
dimethylallyl diphosphate
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pH 8.7, 30°C
0.08
dimethylallyl diphosphate
pH 8.7, 30°C
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8.1 - 9.4
pH 8.1: about 50% of maximal activity, pH 9.4: about 50% of maximal activity
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30
assay at
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-80°C, 2 months, less than 5% loss of activity
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expression in Escherichia coli as a His8-tagged protein
expression in Escherichia coli as a His8-tagged protein
expression in Escherichia coli as a His8-tagged protein
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expression in Escherichia coli as a His8-tagged protein
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Q0CZR3_ASPTN
Aspergillus terreus (strain NIH 2624 / FGSC A1156)
336
37289
TrEMBL
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Haug-Schifferdecker, E.; Arican, D.; Brückner, R.; Heide, L.
A new group of aromatic prenyltransferases in fungi, catalyzing a 2,7-dihydroxynaphthalene 3-dimethylallyl-transferase reaction
J. Biol. Chem.
285
16487-16494
2010
Aspergillus terreus (Q0CZR3), Aspergillus terreus NIHH 2624 (Q0CZR3), Botrytis cinerea, Botrytis cinerea B05.10, Sclerotinia sclerotiorum 1980, Sclerotinia sclerotiorum
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