Information on EC 2.5.1.97 - pseudaminic acid synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.5.1.97
-
RECOMMENDED NAME
GeneOntology No.
pseudaminic acid synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
phosphoenolpyruvate + 2,4-bis(acetylamino)-2,4,6-trideoxy-beta-L-altropyranose + H2O = 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid + phosphate
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Amino sugar and nucleotide sugar metabolism
-
-
CMP-pseudaminate biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
phosphoenolpyruvate:2,4-bis(acetylamino)-2,4,6-trideoxy-beta-L-altropyranose transferase (phosphate-hydrolysing, 2,7-acetylamino-transfering, 2-carboxy-2-oxoethyl-forming)
The enzyme requires a divalent metal ion, the highest activity values are observed in the presence of Mn2+ and Co2+ (10 mM).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
Campylobacter jejuni and Campylobacter coli are the main causes of bacterial diarrhea worldwide. In all of these pathogenic organisms, the flagellin proteins are heavily glycosylated with pseudaminic acid (the product of the PseI catalyzed reaction). The presence of 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid is required for the proper development of the flagella and is thereby necessary for motility in, and invasion of, the host
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
phosphoenolpyruvate + 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + H2O
phosphate + 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid
show the reaction diagram
additional information
?
-
-
N-acetyl-D-mannosamine can not replace 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose as a substrate indicating that the enzyme does not possess detectable sialic acid synthase activity
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phosphoenolpyruvate + 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + H2O
phosphate + 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
pseudaminic acid synthase requires the presence of a divalent metal ion for catalysis. Addition of 10 mM Mg2+ results in 30% of the activity obtained with 10 mM Co2+
Co2+
-
pseudaminic acid synthase requires the presence of a divalent metal ion for catalysis. The highest values are observed in the presence of Mn2+ and Co2+ (10 mM)
Mg2+
-
pseudaminic acid synthase requires the presence of a divalent metal ion for catalysis. Addition of 10 mM Mg2+ results in 48% of the activity obtained with 10 mM Co2+
Mn2+
-
pseudaminic acid synthase requires the presence of a divalent metal ion for catalysis. The highest values are observed in the presence of Mn2+ and Co2+ (10 mM)
Ni2+
-
pseudaminic acid synthase requires the presence of a divalent metal ion for catalysis. Addition of 10 mM Mg2+ results in 26% of the activity obtained with 10 mM Co2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0095
2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose
-
pH 7.4, 25°C
0.0065
phosphoenolpyruvate
-
pH 7.4, 25°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.65
2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose
Campylobacter jejuni
-
pH 7.4, 25°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38647
-
x * 38647, calculated from sequence
38670
-
x * 38670, electrospray ionization-mass spectrometry, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 38647, calculated from sequence; x * 38670, electrospray ionization-mass spectrometry, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
overexpression in Escherichia coli
-