Information on EC 2.5.1.89 - tritrans,polycis-undecaprenyl-diphosphate synthase [geranylgeranyl-diphosphate specific]

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.5.1.89
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RECOMMENDED NAME
GeneOntology No.
tritrans,polycis-undecaprenyl-diphosphate synthase [geranylgeranyl-diphosphate specific]
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
geranylgeranyl diphosphate + 7 isopentenyl diphosphate = 7 diphosphate + tritrans,heptacis-undecaprenyl diphosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Terpenoid backbone biosynthesis
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isoprenoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
geranylgeranyl-diphosphate:isopentenyl-diphosphate cistransferase (adding 7 isopentenyl units)
This enzyme is involved in the biosynthesis of the glycosyl carrier lipid in some archaebacteria. Unlike EC 2.5.1.31, its counterpart in most bacteria, it prefers geranylgeranyl diphosphate to farnesyl diphosphate as the allylic substrate, resulting in production of a tritrans,polycis variant of undecaprenyl diphosphate [1].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene ape_1385
SwissProt
Manually annotated by BRENDA team
gene ape_1385
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
show the reaction diagram
about 35% of the activity with geranylgeranyl diphosphate
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-
?
all-trans-geranylfarnesyl diphosphate + 6 isopentenyl diphosphate
6 diphosphate + hexatrans,tetracis-undecaprenyl diphosphate
show the reaction diagram
geranylgeranyl diphosphate + 7 isopentenyl diphosphate
7 diphosphate + tritrans,heptacis-undecaprenyl diphosphate
show the reaction diagram
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-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
all-trans-geranylfarnesyl diphosphate + 6 isopentenyl diphosphate
6 diphosphate + hexatrans,tetracis-undecaprenyl diphosphate
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
about 10% of the activity with Mg2+
Cu2+
about 5% of the activity with Mg2+
Mn2+
about 20% of the activity with Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ETA
complete inhibition
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Triton X-100
activates
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
1 h, more than 90% of the activity is retained
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene ape_1385, recombinant expression in Escherichia coli strain BL21 (DE3)
isolation of cis-polyprenyl diphosphate synthase, homology with known cis-polyprenyl diphosphate synthases, expression in Escherichia coli
Show AA Sequence (570 entries)
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