Information on EC 2.5.1.80 - 7-dimethylallyltryptophan synthase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.5.1.80
-
RECOMMENDED NAME
GeneOntology No.
7-dimethylallyltryptophan synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dimethylallyl diphosphate + L-tryptophan = diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alkenyl group transfer
-
-
-
-
Friedel-Crafts alkylation
-
-
SYSTEMATIC NAME
IUBMB Comments
dimethylallyl-diphosphate:L-tryptophan 7-dimethylallyltransferase
This enzyme is more flexible towards the aromatic substrate than EC 2.5.1.34 (4-dimethylallyltryptophan synthase), but similar to that enzyme, accepts only dimethylallyl diphosphate as the prenyl donor.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene An13g01840 or ANI_1_660114
UniProt
Manually annotated by BRENDA team
gene An13g01840 or ANI_1_660114
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
metabolism
-
the enzyme is involved in the biosynthesis of astechrome
physiological function
additional information
-
analysis of the structural basis of the regioselective prenylation of L-tryptophan at the C7 position by comparison of the three-dimensional structural models of 7-DMATSNeo with FgaPT2 (4-DMATS), EC 2.5.1.34, from Aspergillus fumigatus. Construction of three-dimensional structural models of 7 DMATSNeo and FgaPT2 (4-DMATS). Docking studies of potential substrates, overview. Residues F129, L130, E138, F226, F243 are involved in binding of L-tryptophan, residue Y393 is involved in stabilization of the allylic cation via cation-Pi interactions. Residues R154, K239, Y241, R311, K313, K391, Y393, K454, and Y460 are involved in diphosphate coordination
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-pentenyl diphosphate + L-tryptophan
diphosphate + 5-(pent-2-enyl)-L-tryptophan
show the reaction diagram
-
C-5 prenylation
-
-
?
benzyl diphosphate + L-tryptophan
diphosphate + 5-benzyl-L-tryptophan
show the reaction diagram
-
C-5 prenylation
-
-
?
dimethylallyl diphosphate + 1,6-dihydroxynaphthalene
diphosphate + 4-(3-methylbut-2-en-1-yl)naphthalene-1,6-diol
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + 1,7-dihydroxynaphthalene
diphosphate + 4-(3-methylbut-2-en-1-yl)naphthalene-1,7-diol
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + 1-methyl-DL-tryptophan
diphosphate + 1-methyl-4-(3-methylbut-2-enyl)-DL-tryptophan
show the reaction diagram
-
-
-
-
-
dimethylallyl diphosphate + 1-methyl-L-tryptophan
diphosphate + 1-methyl-7-(3-methylbut-2-enyl)-L-tryptophan
show the reaction diagram
-
35.8% of the yield with L-tryptophan
-
?
dimethylallyl diphosphate + 1-naphthol
diphosphate + 4-(3-methylbut-2-en-1-yl)naphthalen-1-ol
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + 11-methyl-DL-tryptophan
?
show the reaction diagram
-
tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 33.5% (FgaPT2 followed by 7-DMATS), 4.8% (7-DMATS followed by FgaPT2)
-
-
?
dimethylallyl diphosphate + 11-methyl-DL-tryptophan
diphosphate + 11-methyl-4-(3-methylbut-2-enyl)-DL-tryptophan
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + 11-methyl-DL-tryptophan
diphosphate + 11-methyl-DL-7-(gamma,gamma-dimethylallyl)tryptophan
show the reaction diagram
-
19.1% of the yield with L-tryptophan
-
?
dimethylallyl diphosphate + 2,6-dihydroxynaphthalene
diphosphate + 1-(3-methylbut-2-en-1-yl)naphthalene-2,6-diol
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + 2,7-dihydroxynaphthalene
diphosphate + 1-(3-methylbut-2-en-1-yl)naphthalene-2,7-diol + 3-(3-methylbut-2-en-1-yl)naphthalene-2,7-diol + 7-[(3-methylbut-2-en-1-yl)oxy]naphthalen-2-ol + 1,6-bis(3-methylbut-2-en-1-yl)naphthalene-2,7-diol
show the reaction diagram
-
-
1-(3-methylbut-2-en-1-yl)naphthalene-2,7-diol is the major product
-
?
dimethylallyl diphosphate + 3-iodo-L-tyrosine
diphosphate + 3-iodo-4-(3-methylbut-2-enyl)-L-tyrosine
show the reaction diagram
-
C-4 prenylation
-
-
?
dimethylallyl diphosphate + 4-amino-L-phenylalanine
diphosphate + 4-amino-4-(3-methylbut-2-enyl)-L-phenylalanine
show the reaction diagram
-
C-4 prenylation
-
-
?
dimethylallyl diphosphate + 4-methyl-DL-tryptophan
diphosphate + 4-methyl-4-(3-methylbut-2-enyl)-DL-tryptophan
show the reaction diagram
-
-
-
-
-
dimethylallyl diphosphate + 4-methyl-DL-tryptophan
diphosphate + 4-methyl-7-(3-methylbut-2-enyl)-DL-tryptophan
show the reaction diagram
-
89.4% of the yield with L-tryptophan
-
?
dimethylallyl diphosphate + 4-methyl-DL-tryptophan
diphosphate + 7-(3-methylbut-2-enyl)-4-methyl-DL-tryptophan
show the reaction diagram
dimethylallyl diphosphate + 5-bromo-DL-tryptophan
diphosphate + 5-bromo-7-(3-methylbut-2-enyl)-DL-tryptophan
show the reaction diagram
-
7.2% of the yield with L-tryptophan
-
?
dimethylallyl diphosphate + 5-bromo-L-tryptophan
diphosphate + 5-bromo-4-(3-methylbut-2-enyl)-L-tryptophan
show the reaction diagram
-
low activity
-
-
?
dimethylallyl diphosphate + 5-fluoro-L-tryptophan
diphosphate + 5-fluoro-7-(3-methylbut-2-enyl)-L-tryptophan
show the reaction diagram
-
10.7% of the yield with L-tryptophan
-
?
dimethylallyl diphosphate + 5-hydroxy-L-tryptophan
diphosphate + 5-hydroxy-4-(3-methylbut-2-enyl)-L-tryptophan
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + 5-hydroxy-L-tryptophan
diphosphate + 5-hydroxy-7-(3-methylbut-2-enyl)-L-tryptophan
show the reaction diagram
-
26.7% of the yield with L-tryptophan
-
?
dimethylallyl diphosphate + 5-methoxy-DL-tryptophan
?
show the reaction diagram
-
tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 10.3% (FgaPT2 followed by 7-DMATS), 3.5% (7-DMATS followed by FgaPT2)
-
-
?
dimethylallyl diphosphate + 5-methoxy-DL-tryptophan
diphosphate + 5-methoxy-7-(3-methylbut-2-enyl)-DL-tryptophan
show the reaction diagram
-
13.3% of the yield with L-tryptophan
-
?
dimethylallyl diphosphate + 5-methoxy-DL-tryptophan
diphosphate + 7-(3-methylbut-2-enyl)-5-methoxy-DL-tryptophan
show the reaction diagram
dimethylallyl diphosphate + 5-methyl-DL-tryptophan
?
show the reaction diagram
-
tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 24.3% (FgaPT2 followed by 7-DMATS), 0.9% (7-DMATS followed by FgaPT2)
-
-
?
dimethylallyl diphosphate + 5-methyl-DL-tryptophan
diphosphate + 5-methyl-4-(3-methylbut-2-enyl)-DL-tryptophan
show the reaction diagram
-
-
-
-
-
dimethylallyl diphosphate + 5-methyl-DL-tryptophan
diphosphate + 5-methyl-7-(3-methylbut-2-enyl)-DL-tryptophan
show the reaction diagram
-
74.8% of the yield with L-tryptophan
-
?
dimethylallyl diphosphate + 5-methyl-DL-tryptophan
diphosphate + 7-(3-methylbut-2-enyl)-5-methyl-DL-tryptophan
show the reaction diagram
dimethylallyl diphosphate + 6-fluoro-DL-tryptophan
diphosphate + 6-fluoro-4-(3-methylbut-2-enyl)-DL-tryptophan
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + 6-fluoro-DL-tryptophan
diphosphate + 6-fluoro-7-(3-methylbut-2-enyl)-DL-tryptophan
show the reaction diagram
-
33.7% of the yield with L-tryptophan
-
?
dimethylallyl diphosphate + 6-methyl-DL-tryptophan
?
show the reaction diagram
-
tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 2.6% (FgaPT2 followed by 7-DMATS), 1.5% (7-DMATS followed by FgaPT2)
-
-
?
dimethylallyl diphosphate + 6-methyl-DL-tryptophan
diphosphate + 6-methyl-4-(3-methylbut-2-enyl)-DL-tryptophan
show the reaction diagram
-
low activity
-
-
?
dimethylallyl diphosphate + 6-methyl-DL-tryptophan
diphosphate + 6-methyl-7-(3-methylbut-2-enyl)-DL-tryptophan
show the reaction diagram
-
19.8% of the yield with L-tryptophan
-
?
dimethylallyl diphosphate + 6-methyl-DL-tryptophan
diphosphate + 7-(3-methylbut-2-enyl)-6-methyl-DL-tryptophan
show the reaction diagram
dimethylallyl diphosphate + 7-methyl-DL-tryptophan
diphosphate + 7-(3-methylbut-2-enyl)-DL-tryptophan + ?
show the reaction diagram
-
0.3% of the yield with L-tryptophan
-
?
dimethylallyl diphosphate + apigenin
diphosphate + ?
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + ardeemin fumiquinazoline
diphosphate + (1S,4R)-4-methyl-1-[[6-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione + (1S,4R)-4-methyl-1-[[7-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione
show the reaction diagram
dimethylallyl diphosphate + biochanin A
diphosphate + gancaonin A
show the reaction diagram
-
15.9% activity compared to substrate L-tryptophan
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-Gly
diphosphate + cyclo-L-7-(3-methylbut-2-enyl)-Trp-Gly
show the reaction diagram
-
1.8% of the activity with L-tryptophan
-
?
dimethylallyl diphosphate + D-tryptophan
?
show the reaction diagram
-
tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 4.3% (FgaPT2 followed by 7-DMATS), 2.6% (7-DMATS followed by FgaPT2)
-
-
?
dimethylallyl diphosphate + D-tryptophan
diphosphate + 4-(3-methylbut-2-enyl)-D-tryptophan
show the reaction diagram
-
low activity
-
-
?
dimethylallyl diphosphate + D-tryptophan
diphosphate + D-7-(3-methylbut-2-enyl)-tryptophan
show the reaction diagram
dimethylallyl diphosphate + dia,ent-ardeemin fumiquinazoline
diphosphate + (1R,4R)-4-methyl-1-[[6-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione + (1R,4R)-4-methyl-1-[[7-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione
show the reaction diagram
dimethylallyl diphosphate + dia-ardeemin fumiquinazoline
diphosphate + (1S,4S)-4-methyl-1-[[6-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione + (1S,4S)-4-methyl-1-[[7-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + DL-indole-3-lactic acid
?
show the reaction diagram
dimethylallyl diphosphate + DL-indole-3-lactic acid
diphosphate + 7-(3-methylbut-2-enyl)-DL-indole-3-lactic acid
show the reaction diagram
-
4.3% of the yield with L-tryptophan
-
?
dimethylallyl diphosphate + ent-ardeemin fumiquinazoline
diphosphate + (1R,4S)-4-methyl-1-[[7-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]methyl]-2H-pyrazino[2,1-b]quinazoline-3,6(1H,4H)-dione
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + eriodictyol
diphosphate + 6-(3-methylbut-2-enyl)eriodictyol
show the reaction diagram
-
19.0% activity compared to substrate L-tryptophan
-
-
?
dimethylallyl diphosphate + genistein
diphosphate + wighteone
show the reaction diagram
-
10.4% activity compared to substrate L-tryptophan
-
-
?
dimethylallyl diphosphate + hesperetin
diphosphate + sigmoidin B
show the reaction diagram
-
1.4% activity compared to substrate L-tryptophan
-
-
?
dimethylallyl diphosphate + indole-3-acetic acid
diphosphate + 7-(3-methylbut-2-enyl)-indole-3-acetic acid
show the reaction diagram
-
10.8% of the yield with L-tryptophan
-
?
dimethylallyl diphosphate + indole-3-butyric acid
diphosphate + 7-(3-methylbut-2-enyl)-indole-3-butyric acid
show the reaction diagram
-
9.1% of the yield with L-tryptophan
-
?
dimethylallyl diphosphate + indole-3-propionic acid
?
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + indole-3-propionic acid
diphosphate + 7-(3-methylbut-2-enyl)-indole-3-propionic acid
show the reaction diagram
-
8.5% of the yield with L-tryptophan
-
?
dimethylallyl diphosphate + indole-3-pyruvic acid
diphosphate + 7-(3-methylbut-2-enyl)-indole-3-pyruvic acid
show the reaction diagram
-
4.8% of the yield with L-tryptophan
-
?
dimethylallyl diphosphate + L-abrine
?
show the reaction diagram
dimethylallyl diphosphate + L-abrine
diphosphate + ?
show the reaction diagram
dimethylallyl diphosphate + L-beta-homo-L-tryptophan
?
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + L-beta-homotryptophan
?
show the reaction diagram
-
tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 31.5% (FgaPT2 followed by 7-DMATS), 6.6% (7-DMATS followed by FgaPT2)
-
-
?
dimethylallyl diphosphate + L-beta-homotryptophan
diphosphate + 7-(3-methylbut-2-enyl)-L-beta-homotryptophan
show the reaction diagram
dimethylallyl diphosphate + L-o-tyrosine
diphosphate + 4-(3-methylbut-2-enyl)-L-o-tyrosine
show the reaction diagram
-
C-4 prenylation
4-dimethylallyl-L-o-tyrosine
-
?
dimethylallyl diphosphate + L-o-tyrosine
diphosphate + 5-(3-methylbut-2-enyl)-L-o-tyrosine
show the reaction diagram
-
C-5 prenylation, proposed reaction mechanism, overview
-
-
?
dimethylallyl diphosphate + L-Trp-Gly
diphosphate + L-7-(3-methylbut-2-enyl)-Trp-Gly
show the reaction diagram
-
10.9% of the activity with L-tryptophan
-
?
dimethylallyl diphosphate + L-tryptophan
?
show the reaction diagram
-
tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 89.7% (FgaPT2 followed by 7-DMATS), 20% (7-DMATS followed by FgaPT2)
-
-
?
dimethylallyl diphosphate + L-tryptophan
diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan
show the reaction diagram
dimethylallyl diphosphate + L-tryptophan
diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan
show the reaction diagram
dimethylallyl diphosphate + L-tryptophan
diphosphate + L-7-(3-methylbut-2-enyl)-tryptophan
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + L-tryptophan hydroxamate
diphosphate + L-7-(gamma,gamma-dimethylallyl)tryptophan hydroxamate
show the reaction diagram
-
4.6% of the yield with L-tryptophan
-
?
dimethylallyl diphosphate + L-tryptophan methyl ester
diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan methyl ester
show the reaction diagram
dimethylallyl diphosphate + L-tyrosine
diphosphate + 5-[(2E)-pent-2-en-1-yl]-L-tryptophan
show the reaction diagram
-
O-4 prenylation
-
-
?
dimethylallyl diphosphate + luteolin
diphosphate + ?
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + N-acetyl-DL-tryptophan
diphosphate + 7-(3-methylbut-2-enyl)-N-acetyl-DL-tryptophan
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + N-acetyl-DL-tryptophan
diphosphate + N-acetyl-4-(3-methylbut-2-enyl)-DL-tryptophan
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + N-acetyl-DL-tryptophan
diphosphate + N-acetyl-DL-7-(gamma,gamma-dimethylallyl)tryptophan
show the reaction diagram
-
7.7% of the yield with L-tryptophan
-
?
dimethylallyl diphosphate + Nalpha-methyl-L-tryptophan
diphosphate + Nalpha-methyl-L-7-(gamma,gamma-dimethylallyl)tryptophan
show the reaction diagram
i.e. L-abrine
82.2% of the yield with L-tryptophan
-
?
dimethylallyl diphosphate + naringenin
diphosphate + 6-(3-methylbut-2-enyl)naringenin
show the reaction diagram
-
1.4% activity compared to substrate L-tryptophan
-
-
?
dimethylallyl diphosphate + phloretin
diphosphate + 6-dimethylallyl-5,7,9,1'-tetrahydroxydihydrochalcone
show the reaction diagram
-
17.4% activity compared to substrate L-tryptophan
-
-
?
dimethylallyl diphosphate + tryptamine
?
show the reaction diagram
-
tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 11% (FgaPT2 followed by 7-DMATS), 6.3% (7-DMATS followed by FgaPT2)
-
-
?
dimethylallyl diphosphate + tryptamine
diphosphate + 7-(3-methylbut-2-enyl)-tryptamine
show the reaction diagram
methylallyl diphosphate + L-tryptophan
diphosphate + 4-(but-2-enyl)-L-tryptophan
show the reaction diagram
-
C-4 and C-5 prenylation
-
-
?
methylallyl diphosphate + L-tryptophan
diphosphate + 5-(but-2-enyl)-L-tryptophan
show the reaction diagram
-
C-4 and C-5 prenylation
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dimethylallyl diphosphate + L-tryptophan
diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
strong inhibition
phloretin
-
shows substrate inhibition at 1 mM or higher concentrations
Zn2+
slight inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.416
5-methyl-DL-tryptophan
-
pH 7.5, 37°C, recombinant enzyme
0.1
ardeemin fumiquinazoline
-
pH 7.5, 37°C
-
0.07
Biochanin A
-
pH not specified in the publication, 37°C
0.34
dia,ent-ardeemin fumiquinazoline
-
pH 7.5, 37°C
-
0.18
dia-ardeemin fumiquinazoline
-
pH 7.5, 37°C
-
0.052 - 0.71
dimethylallyl diphosphate
0.14
ent-ardeemin fumiquinazoline
-
pH 7.5, 37°C
-
1.26
eriodictyol
-
pH not specified in the publication, 37°C
0.16
genistein
-
pH not specified in the publication, 37°C
1.1
hesperetin
-
pH not specified in the publication, 37°C
0.081
L-abrine
-
pH 7.5, 37°C, recombinant enzyme
0.17
L-o-tyrosine
-
pH 7.5, 37°C, recombinant enzyme
-
0.137 - 0.238
L-tryptophan
2.876
L-tryptophan methyl ester
-
pH 7.5, 37°C, recombinant enzyme
0.99
naringenin
-
pH not specified in the publication, 37°C
0.13
phloretin
-
pH not specified in the publication, 37°C
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.125
5-methyl-DL-tryptophan
Neosartorya sp.
-
pH 7.5, 37°C, recombinant enzyme
0.079
ardeemin fumiquinazoline
Aspergillus fumigatus
-
pH 7.5, 37°C
-
0.019
Biochanin A
Aspergillus fumigatus
-
pH not specified in the publication, 37°C
0.002
dia,ent-ardeemin fumiquinazoline
Aspergillus fumigatus
-
pH 7.5, 37°C
-
0.053
dia-ardeemin fumiquinazoline
Aspergillus fumigatus
-
pH 7.5, 37°C
-
0.092
dimethylallyl diphosphate
Neosartorya sp.
-
pH 7.5, 37°C, recombinant enzyme
0.059
ent-ardeemin fumiquinazoline
Aspergillus fumigatus
-
pH 7.5, 37°C
-
0.39
eriodictyol
Aspergillus fumigatus
-
pH not specified in the publication, 37°C
0.027
genistein
Aspergillus fumigatus
-
pH not specified in the publication, 37°C
0.026
hesperetin
Aspergillus fumigatus
-
pH not specified in the publication, 37°C
0.043
L-abrine
Neosartorya sp.
-
pH 7.5, 37°C, recombinant enzyme
0.045
L-o-tyrosine
Aspergillus fumigatus
-
pH 7.5, 37°C, recombinant enzyme
-
0.0053 - 0.23
L-tryptophan
0.112
L-tryptophan methyl ester
Neosartorya sp.
-
pH 7.5, 37°C, recombinant enzyme
0.023
naringenin
Aspergillus fumigatus
-
pH not specified in the publication, 37°C
0.036
phloretin
Aspergillus fumigatus
-
pH not specified in the publication, 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.3
5-methyl-DL-tryptophan
Neosartorya sp.
-
pH 7.5, 37°C, recombinant enzyme
4342
0.261
Biochanin A
Aspergillus fumigatus
-
pH not specified in the publication, 37°C
1435
1.76
dimethylallyl diphosphate
Neosartorya sp.
-
pH 7.5, 37°C, recombinant enzyme
157
0.312
eriodictyol
Aspergillus fumigatus
-
pH not specified in the publication, 37°C
2485
0.171
genistein
Aspergillus fumigatus
-
pH not specified in the publication, 37°C
377
0.024
hesperetin
Aspergillus fumigatus
-
pH not specified in the publication, 37°C
2081
0.542
L-abrine
Neosartorya sp.
-
pH 7.5, 37°C, recombinant enzyme
27257
0.265
L-o-tyrosine
Aspergillus fumigatus
-
pH 7.5, 37°C, recombinant enzyme
214008
0.47 - 1.643
L-tryptophan
119
0.038
L-tryptophan methyl ester
Neosartorya sp.
-
pH 7.5, 37°C, recombinant enzyme
7483
0.023
naringenin
Aspergillus fumigatus
-
pH not specified in the publication, 37°C
586
0.286
phloretin
Aspergillus fumigatus
-
pH not specified in the publication, 37°C
1762
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 37
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000
1 * 50000, SDS-PAGE, 1 * 54000, calculated, His-tagged recombinant protein
53000
-
x * 53000, calculated
54000
1 * 50000, SDS-PAGE, 1 * 54000, calculated, His-tagged recombinant protein
65000
gel filtration
228000
recombinant His6-tagged enzyme, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
1 * 50000, SDS-PAGE, 1 * 54000, calculated, His-tagged recombinant protein
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
the purified recombinant enzyme retains 90% activity after 240 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
37°C, absence of substrate, 16 h, 80% residual activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
-
recombinant His-tagged 7-DMATS from Escherichia coli strains XL1 Blue or M15 or from Saccharomyces cerevisiae strain INVSc1 by nickel affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli strain BL21 to near homogeneity by nickel affinity chromatography and desalting gel filtration
recombinant His6-tagged enzyme from Escherichia coli strain XL-1 Blue MRF' by nickel affinity chromatography to homogeneity
-
recombinant protein
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
7-DMATS expression in Escherichia coli
-
DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His6-tagged enzyme in Escherichia coli strain XL-1 Blue MRF' from vector pQE30-dmatsNeo
-
expressed as a recombinant protein
-
expression in Escherichia coli, His-tagged protein
-
expression of His-tagged 7-DMATS in Escherichia coli strains XL1 Blue or M15 or in Saccharomyces cerevisiae strain INVSc1
gene An13g01840, DNA and amino acid sequence determination and analysis, overexpression of His6-tagged enzyme in Escherichia coli strain SoluBL21, subcloning in Escherichia coli strain XL1 BlueMRF'
recombinant enzyme overexpression
-
recombinant overexpression
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
-
the combination of the two dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) can be successfully used for chemoenzymatic synthesis of the diprenylated derivatives. The potential of recombinant enzymes from secondary metabolite biosynthesis as promising tools for the production of designed compounds is demonstrated
drug development
synthesis