Information on EC 2.5.1.76 - cysteate synthase

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The expected taxonomic range for this enzyme is: Methanosarcina acetivorans

EC NUMBER
COMMENTARY hide
2.5.1.76
-
RECOMMENDED NAME
GeneOntology No.
cysteate synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
O-phospho-L-serine + sulfite = L-cysteate + phosphate
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
coenzyme M biosynthesis II
-
-
coenzyme M biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
sulfite:O-phospho-L-serine sulfotransferase (phosphate-hydrolysing, L-cysteate-forming)
A pyridoxal-phosphate protein. It is highly specific for O-phospho-L-serine and sulfite. The reaction proceeds through a dehydroalanine (2-aminoacrylic acid) intermediate. The enzyme from Methanosarcina acetivorans is evolutionarily related to threonine synthase (EC 4.2.3.1), but the reaction is more similar to that of O-phosphoserine sulfhydrylase (EC 2.5.1.65).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-phosphoserine + sulfite
L-cysteate + phosphate
show the reaction diagram
-
-
-
?
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
DL-2-amino-3-phosphonopropionate
-
DL-2-Amino-4-phosphonobutanoate
-
DL-2-amino-5-phosphonopentanoate
1 mM, more than 80% reemaining activity
L-lysine
1 mM, more than 80% reemaining activity
L-threonine
1 mM, more than 80% reemaining activity
O-acetyl-L-serine
1 mM, more than 80% reemaining activity
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.43
L-phosphoserine
pH 7.0, 37C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.18
L-phosphoserine
Methanosarcina acetivorans
Q8TKU7
pH 7.0, 37C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.14
DL-2-amino-3-phosphonopropionate
Methanosarcina acetivorans
Q8TKU7
pH 7.0, 37C
16.5
DL-2-Amino-4-phosphonobutanoate
Methanosarcina acetivorans
Q8TKU7
pH 7.0, 37C
15.9
L-aspartate
Methanosarcina acetivorans
Q8TKU7
pH 7.0, 37C
12.6
L-cysteate
Methanosarcina acetivorans
Q8TKU7
pH 7.0, 37C
37.1
L-serine
Methanosarcina acetivorans
Q8TKU7
pH 7.0, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
132000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trimer
3 * 46000, SDS-PAGE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression of truncated protein lacking the first 43 codons of the gene results in an insoluble protein. The refolded protein catalyzes the elimination of phosphate from L-phosphoserine with a rate of 3% of the full-length protein