Information on EC 2.5.1.53 - uracilylalanine synthase

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The expected taxonomic range for this enzyme is: fabids

EC NUMBER
COMMENTARY hide
2.5.1.53
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RECOMMENDED NAME
GeneOntology No.
uracilylalanine synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
O-acetyl-L-serine + uracil = 3-(uracil-1-yl)-L-alanine + acetate
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
condensing reaction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
willardiine and isowillardiine biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
O3-acetyl-L-serine:uracil 1-(2-amino-2-carboxyethyl)transferase
The enzyme produces the non-proteinogenic amino acid L-willardiine, which is naturally found in the plants Acacia willardiana, Mimosa pigra, and Pisum sativum (pea). The enzyme from Pisum species also produces L-isowillardiine. Not identical with EC 2.5.1.47 cysteine synthase.
CAS REGISTRY NUMBER
COMMENTARY hide
113573-73-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
no activity in Citrullus vulgaris
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-
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Manually annotated by BRENDA team
no activity in Fagus japonica
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-
-
Manually annotated by BRENDA team
no activity in Lupinus luteus
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-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 O3-acetyl-L-serine + 2 uracil
3-(uracil-1-yl)-L-alanine + 3-(uracil-3-yl)-L-alanine + 2 acetate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 O3-acetyl-L-serine + 2 uracil
3-(uracil-1-yl)-L-alanine + 3-(uracil-3-yl)-L-alanine + 2 acetate
show the reaction diagram
-
involved in biosynthesis of heterocyclic beta-substituted alanines
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2,4-Dihydroxypyrimidin-1-yl)-L-alanine
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i.e. willardiine or 3-(uracil-1-yl)-L-alanine, 30 mM, 84% inhibition of willardiine synthesis and 96% inhibition of isowillardiine synthesis
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(2,4-Dihydroxypyrimidin-3-yl)-L-alanine
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i.e. isowillardiine or 3-(uracil-3-yl)-L-alanine, 30 mM produces 75% inhibition of willardiine synthesis and 93% inhibition of synthesis of isowillardiine
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hydroxylamine
PCMB
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0.001 mM, about 90% inhibition
pyridoxal 5'-phosphate
Tris-HCl buffer
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Uracil
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.3 - 10
Uracil
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8 - 7.9
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8.2
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about half-maximal activity at pH 7.5 and 8.2
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000
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gel filtration
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 50
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2 min, slow decrease of activity
50 - 60
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2 min, rapid decrease of activity
70
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2 min, all activity ceased
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, partially purified enzyme preparation, 30% loss of activity within 24 h, complete inactivation after 48 h
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liquid N2, crude enzyme preparation, in 3 M ammonium sulfate, 30% loss of activity within 8 weeks, beyond 8 weeks rapid decline of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
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