Information on EC 2.5.1.41 - phosphoglycerol geranylgeranyltransferase

Word Map on EC 2.5.1.41
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.5.1.41
-
RECOMMENDED NAME
GeneOntology No.
phosphoglycerol geranylgeranyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
geranylgeranyl diphosphate + sn-glycerol 1-phosphate = diphosphate + 3-(O-geranylgeranyl)-sn-glycerol 1-phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alkenyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
CDP-archaeol biosynthesis
-
-
Glycerophospholipid metabolism
-
-
lipid metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
geranylgeranyl diphosphate:sn-glyceryl phosphate geranylgeranyltransferase
This cytosolic enzyme catalyses the first pathway-specific step in the biosynthesis of the core membrane diether lipids in archaebacteria [2]. Requires Mg2+ for maximal activity [2]. It catalyses the alkylation of the primary hydroxy group in sn-glycerol 1-phosphate by geranylgeranyl diphosphate (GGPP) in a prenyltransfer reaction where a hydroxy group is the nucleophile in the acceptor substrate [2]. The other enzymes involved in the biosynthesis of polar lipids in Archaea are EC 1.1.1.261 (sn-glycerol-1-phosphate dehydrogenase), EC 2.5.1.42 (geranylgeranylglycerol-phosphate geranylgeranyltransferase) and EC 2.7.7.67 (CDP-archaeol synthase), which lead to the formation of CDP-unsaturated archaeol. The final step in the pathway involves the addition of L-serine, with concomitant removal of CMP, leading to the production of unsaturated archaetidylserine [5].
CAS REGISTRY NUMBER
COMMENTARY hide
124650-69-7
-
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
-
diversity of thaumarchaeotal GGGP synthases in the marine water column, phylogenetic analysis and tree and correlation to the classification of shallow and deep water clusters, overview. Differences in isoprenoid glycerol dialkyl glycerol tetraethers distributions from subsurface to deep waters that may be explained by differences in the GGGP synthase, suggesting a genetic control on isoprenoid glycerol dialkyl glycerol tetraethers distribution. Diversity of archaeal GGGP synthases, overview
metabolism
-
key enzyme of the thaumarchaeotal lipid biosynthetic pathway
physiological function
-
the key enzyme catalyses the formation of an ether bond between isoprenyl diphosphate and glycerol-1-phosphate to form 3-(O-geranylgeranyl)-sn-glycerol 1-phosphate, and is the first committed step towards ether membrane lipid synthesis
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
geranylgeranyl diphosphate + sn-glycerol 1-phosphate
?
show the reaction diagram
-
-
-
-
?
geranylgeranyl diphosphate + sn-glycerol 1-phosphate
diphosphate + 3-(O-geranylgeranyl)-sn-glycerol 1-phosphate
show the reaction diagram
-
-
-
-
?
geranylgeranyl diphosphate + sn-glycerol 1-phosphate
diphosphate + 3-O-(geranylgeranyl)glyceryl 1-phosphate
show the reaction diagram
-
-
-
-
?
geranylgeranyl diphosphate + sn-glyceryl phosphate
diphosphate + sn-3-O-(geranylgeranyl)glyceryl 1-phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
geranylgeranyl diphosphate + sn-glycerol 1-phosphate
diphosphate + 3-(O-geranylgeranyl)-sn-glycerol 1-phosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Triton X-100
-
required for enzymatic activity, optimal concentration: 0.01%
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000075 - 0.0041
geranylgeranyl diphosphate
0.0212 - 0.041
sn-glycerol 1-phosphate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
-
assay at and optimal temperature
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50 - 65
-
maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27000
-
SDS-PAGE
50000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
x-ray crystallography
homodimer
-
gel filtration, 2 * 27000
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapour diffusion method using 100 mM citrate, pH 6.0, 10 mM MgCl2, 4-10% polyethylene glycol 4000
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Superdex 200 gel filtration and nickel column chromatography
-
using ammonium sulfate precipitation, four chromatographic steps (DE52, Q-Sepharose, phenyl-Superose, and Protein Pak)
-
using DEAE-cellulose column chromatography, NH4SO4 precipitation, Phenyl-650S and Resourse Q columns, Mini Q column and Superdex 200 HR 10/30 column
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
diversity of thaumarchaeotal GGGP synthases in the marine water column, phylogenetic analysis and tree and correlation to the classification of shallow and deep water clusters, overview
-
expressed in Escherichia coli BL21(DE3) cells
-
recombinantly expressed in Escherichia coli
-
Show AA Sequence (895 entries)
Please use the Sequence Search for a specific query.