Information on EC 2.5.1.35 - aspulvinone dimethylallyltransferase

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The expected taxonomic range for this enzyme is: Aspergillus terreus

EC NUMBER
COMMENTARY hide
2.5.1.35
-
RECOMMENDED NAME
GeneOntology No.
aspulvinone dimethylallyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 dimethylallyl diphosphate + aspulvinone E = 2 diphosphate + aspulvinone H
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alkenyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
dimethylallyl-diphosphate:aspulvinone-E dimethylallyltransferase
This enzyme will also use as acceptor aspulvinone G, a hydroxylated derivative of the complex phenolic pigment aspulvinone E.
CAS REGISTRY NUMBER
COMMENTARY hide
67584-68-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
IAM 2054
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(E)-3-methylpent-2-enyl diphosphate + aspulvinone E
?
show the reaction diagram
(E)-butenyl diphosphate + aspulvinone E
?
show the reaction diagram
cycloheptylideneethyl diphosphate + aspulvinone E
?
show the reaction diagram
cyclohexylideneethyl diphosphate + aspulvinone E
?
show the reaction diagram
cyclopentylideneethyl diphosphate + aspulvinone E
?
show the reaction diagram
dimethylallyl diphosphate + aspulvinone E
diphosphate + aspulvinone H
show the reaction diagram
dimethylallyl diphosphate + aspulvinone G
?
show the reaction diagram
dimethylallyl diphosphate + aspulvinone I
?
show the reaction diagram
-
75% of the activity with aspulvinone E
-
-
?
dimethylallyl diphosphate + aspulvinone I-c
?
show the reaction diagram
-
13% of the activity with aspulvinone E
-
-
?
dimethylallyl diphosphate + atromentic acid
?
show the reaction diagram
-
7% of the activity with aspulvinone E
-
-
?
dimethylallyl diphosphate + pulvinone
?
show the reaction diagram
-
6% of the activity with aspulvinone E
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2SO4
-
-
Aspulvinone E dimethyl ether
-
-
Bromophenol blue
-
-
diphosphate
isopentenyl diphosphate
-
-
lecithin
-
0.025%, 69% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Triton X-100
-
0.025%, activates 1.5times
Tween 80
-
0.025%, activates 2.3times
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0137
aspulvinone E
-
-
0.0077
aspulvinone G
-
-
0.04
dimethylallyl diphosphate
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0178 - 0.05
diphosphate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45000
-
6 * 45000, SDS-PAGE
240000 - 270000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
addition of 2-mercaptoethanol does not affect stability
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable for 1 week
-
4°C, 50% loss of activity after 3 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE