Information on EC 2.5.1.127 - caldopentamine synthase

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The expected taxonomic range for this enzyme is: Hyperthermus butylicus

EC NUMBER
COMMENTARY hide
2.5.1.127
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RECOMMENDED NAME
GeneOntology No.
caldopentamine synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl 3-(methylthio)propylamine + norspermine = S-methyl-5'-thioadenosine + caldopentamine
show the reaction diagram
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
polyamine pathway
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl 3-(methylthio)propylamine:norspermine 3-aminopropyltransferase
The enzyme, characterized from the thermophilic archaeon Hyperthermus butylicus, can also synthesize norspermine from norspermidine and thermospermine from spermidine (with lower activity). The long-chain polyamines stabilize double-stranded DNA at high temperatures. In contrast to EC 2.5.1.23, sym-norspermidine synthase and EC 2.5.1.126, norspermine synthase, this enzyme shows no activity with propane-1,3-diamine.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 S-adenosyl 3-(methylthio)propylamine + caldopentamine
S-methyl-5'-thioadenosine + caldoheptamine
show the reaction diagram
S-adenosyl 3-(methylthio)propylamine + caldopentamine
S-methyl-5'-thioadenosine + caldohexamine
show the reaction diagram
S-adenosyl 3-(methylthio)propylamine + norspermidine
S-methyl-5'-thioadenosine + norspermine
show the reaction diagram
S-adenosyl 3-(methylthio)propylamine + norspermine
S-methyl-5'-thioadenosine + caldopentamine
show the reaction diagram
S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine
S-methyl-5'-thioadenosine + norspermidine
show the reaction diagram
at 2% of the specific activity compared to norspermine
-
-
?
S-adenosyl 3-(methylthio)propylamine + spermidine
S-methyl-5'-thioadenosine + thermospermine + H+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 S-adenosyl 3-(methylthio)propylamine + caldopentamine
S-methyl-5'-thioadenosine + caldoheptamine
show the reaction diagram
S-adenosyl 3-(methylthio)propylamine + caldopentamine
S-methyl-5'-thioadenosine + caldohexamine
show the reaction diagram
S-adenosyl 3-(methylthio)propylamine + norspermidine
S-methyl-5'-thioadenosine + norspermine
show the reaction diagram
S-adenosyl 3-(methylthio)propylamine + norspermine
S-methyl-5'-thioadenosine + caldopentamine
show the reaction diagram
A2BIX4
the long-chain polyamines stabilize double-stranded DNA at high temperatures
-
-
?
S-adenosyl 3-(methylthio)propylamine + spermidine
S-methyl-5'-thioadenosine + thermospermine + H+
show the reaction diagram
A2BIX4
spermidine is not the most preferred substrate for any enzyme. Nevertheless, in vivo the enzyme can be responsible for the synthesis of thermospermine, if spermidine is present in a high concentration in competition to other accepted polyamines
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-
?
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.002
pH 7.5, 95C, substrate: propane-1,3-diamine
0.018
pH 7.5, 95C, substrate: spermidine
0.064
pH 7.5, 95C, substrate: norspermidine
0.112
pH 7.5, 95C, substrate: norspermine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32400
x * 32400, calculation from sequence
35000
x * 35000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli with an N-terminal 6* His sequence