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dimethylallyl diphosphate + flaviolin
?
geranyl diphosphate + 1,3-dihydroxynaphthalene
?
geranyl diphosphate + 2-O-methyl-3-methylflaviolin
diphosphate + (E)-7-((3,7-dimethylocta-2,6-dien-1-yl)oxy)-5-hydroxy-2-methoxy-3-methylnaphthalene-1,4-dione + 6-prenyl-2-methoxy-3-methylflaviolin
geranyl diphosphate + 2-O-methyl-3-methylflaviolin
diphosphate + 6-(3,7-dimethylocta-1,6-dien-3-yl)-5,7-dihydroxy-2-methoxy-3-methylnaphthalene-1,4-dione
geranyl diphosphate + flaviolin
diphosphate + 2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-3,6,7-trihydroxynaphthalene-1,4-dione
-
i.e. 2,5,7-trihydroxynaphthalene-1,4-dione. The kcat/Km of the prenylation of flaviolin is significantly higher than that of the prenylation of the natural substrate 2-O-methyl-3-methylflaviolin
-
-
?
geranyl diphosphate + flaviolin
diphosphate + 3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-2,5,7-trihydroxynaphthalene-1,4-dione
dimethylallyl diphosphate + flaviolin
?
-
-
-
-
?
dimethylallyl diphosphate + flaviolin
?
-
-
-
-
?
geranyl diphosphate + 1,3-dihydroxynaphthalene
?
-
-
-
-
?
geranyl diphosphate + 1,3-dihydroxynaphthalene
?
-
-
-
-
?
geranyl diphosphate + 2-O-methyl-3-methylflaviolin
diphosphate + (E)-7-((3,7-dimethylocta-2,6-dien-1-yl)oxy)-5-hydroxy-2-methoxy-3-methylnaphthalene-1,4-dione + 6-prenyl-2-methoxy-3-methylflaviolin
-
the enzyme is involved in biosynthesis of the polyketide-isoprenoid furaquinocin. It catalyses the transfer of a geranyl group to 2-O-methyl-3-methyl-flaviolin to yield 6-(3,7-dimethylocta-1,6-dien-3-yl)-5,7-dihydroxy-2-methoxy-3-methylnaphthalene-1,4-dione and 7-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]-5-hydroxy-2-methoxy-3-methylnaphthalene-1,4-dione in a 10:1 ratio
-
-
?
geranyl diphosphate + 2-O-methyl-3-methylflaviolin
diphosphate + (E)-7-((3,7-dimethylocta-2,6-dien-1-yl)oxy)-5-hydroxy-2-methoxy-3-methylnaphthalene-1,4-dione + 6-prenyl-2-methoxy-3-methylflaviolin
-
the enzyme is involved in biosynthesis of the polyketide-isoprenoid furaquinocin. It catalyses the transfer of a geranyl group to 2-O-methyl-3-methyl-flaviolin to yield 6-(3,7-dimethylocta-1,6-dien-3-yl)-5,7-dihydroxy-2-methoxy-3-methylnaphthalene-1,4-dione and 7-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]-5-hydroxy-2-methoxy-3-methylnaphthalene-1,4-dione in a 10:1 ratio
-
-
?
geranyl diphosphate + 2-O-methyl-3-methylflaviolin
diphosphate + 6-(3,7-dimethylocta-1,6-dien-3-yl)-5,7-dihydroxy-2-methoxy-3-methylnaphthalene-1,4-dione
-
the enzyme catalyses the transfer of a geranyl group to 2-O-methyl-3-methyl-flaviolin to yield 6-(3,7-dimethylocta-1,6-dien-3-yl)-5,7-dihydroxy-2-methoxy-3-methylnaphthalene-1,4-dione and 7-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]-5-hydroxy-2-methoxy-3-methylnaphthalene-1,4-dione in a 10:1 ratio
-
-
?
geranyl diphosphate + 2-O-methyl-3-methylflaviolin
diphosphate + 6-(3,7-dimethylocta-1,6-dien-3-yl)-5,7-dihydroxy-2-methoxy-3-methylnaphthalene-1,4-dione
-
the enzyme catalyses the transfer of a geranyl group to 2-O-methyl-3-methyl-flaviolin to yield 6-(3,7-dimethylocta-1,6-dien-3-yl)-5,7-dihydroxy-2-methoxy-3-methylnaphthalene-1,4-dione and 7-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]-5-hydroxy-2-methoxy-3-methylnaphthalene-1,4-dione in a 10:1 ratio
-
-
?
geranyl diphosphate + flaviolin
diphosphate + 3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-2,5,7-trihydroxynaphthalene-1,4-dione
-
-
-
-
?
geranyl diphosphate + flaviolin
diphosphate + 3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-2,5,7-trihydroxynaphthalene-1,4-dione
-
-
-
-
?
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geranyl diphosphate + 2-O-methyl-3-methylflaviolin
diphosphate + (E)-7-((3,7-dimethylocta-2,6-dien-1-yl)oxy)-5-hydroxy-2-methoxy-3-methylnaphthalene-1,4-dione + 6-prenyl-2-methoxy-3-methylflaviolin
geranyl diphosphate + 2-O-methyl-3-methylflaviolin
diphosphate + (E)-7-((3,7-dimethylocta-2,6-dien-1-yl)oxy)-5-hydroxy-2-methoxy-3-methylnaphthalene-1,4-dione + 6-prenyl-2-methoxy-3-methylflaviolin
-
the enzyme is involved in biosynthesis of the polyketide-isoprenoid furaquinocin. It catalyses the transfer of a geranyl group to 2-O-methyl-3-methyl-flaviolin to yield 6-(3,7-dimethylocta-1,6-dien-3-yl)-5,7-dihydroxy-2-methoxy-3-methylnaphthalene-1,4-dione and 7-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]-5-hydroxy-2-methoxy-3-methylnaphthalene-1,4-dione in a 10:1 ratio
-
-
?
geranyl diphosphate + 2-O-methyl-3-methylflaviolin
diphosphate + (E)-7-((3,7-dimethylocta-2,6-dien-1-yl)oxy)-5-hydroxy-2-methoxy-3-methylnaphthalene-1,4-dione + 6-prenyl-2-methoxy-3-methylflaviolin
-
the enzyme is involved in biosynthesis of the polyketide-isoprenoid furaquinocin. It catalyses the transfer of a geranyl group to 2-O-methyl-3-methyl-flaviolin to yield 6-(3,7-dimethylocta-1,6-dien-3-yl)-5,7-dihydroxy-2-methoxy-3-methylnaphthalene-1,4-dione and 7-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]-5-hydroxy-2-methoxy-3-methylnaphthalene-1,4-dione in a 10:1 ratio
-
-
?
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0.32
1,3-dihydroxynaphthalene
-
pH 7.5, 30°C, cosubstrate geranyl diphosphate
0.054
2-O-methyl-3-methylflaviolin
-
pH 7.5, 30°C, cosubstrate geranyl diphosphate
0.72
dimethylallyl diphosphate
-
pH 7.5, 30°C, cosubstrate: flaviolin
0.059 - 0.098
geranyl diphosphate
0.048
flaviolin
-
pH 7.5, 30°C, cosubstrate geranyl diphosphate
0.35
flaviolin
-
pH 7.5, 30°C, cosubstrate dimethylallyl diphosphate
0.059
geranyl diphosphate
-
pH 7.5, 30°C, cosubstrate: flaviolin
0.063
geranyl diphosphate
-
pH 7.5, 30°C, cosubstrate: 1,3-dihydroxynaphthalene
0.098
geranyl diphosphate
-
pH 7.5, 30°C, cosubstrate: 2-methoxy-3-methylflaviolin
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