Information on EC 2.5.1.114 - tRNAPhe (4-demethylwyosine37-C7) aminocarboxypropyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
2.5.1.114
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RECOMMENDED NAME
GeneOntology No.
tRNAPhe (4-demethylwyosine37-C7) aminocarboxypropyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + 4-demethylwyosine37 in tRNAPhe = S-methyl-5'-thioadenosine + 7-[(3S)-3-amino-3-carboxypropyl]-4-demethylwyosine37 in tRNAPhe
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
7-(3-amino-3-carboxypropyl)-wyosine biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:tRNAPhe (4-demethylwyosine37-C7) [(3S)-3-amino-3-carboxypropyl]transferase
The enzyme, which is found in all eukaryotes and in the majority of Euryarchaeota (but not in the Crenarchaeota), is involved in the hypermodification of the guanine nucleoside at position 37 of tRNA leading to formation of assorted wye bases. This modification is essential for translational reading-frame maintenance. The eukaryotic enzyme is involved in biosynthesis of the tricyclic base wybutosine, which is found only in tRNAPhe.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
a deletion mutant of the YML005w gene accumulates 4-demethylwyosine
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 4-demethylwyosine37 in tRNAPhe
S-methyl-5'-thioadenosine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 4-demethylwyosine37 in tRNAPhe
S-methyl-5'-thioadenosine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe
show the reaction diagram
PDB
SCOP
CATH
ORGANISM
UNIPROT
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystals of the enzyme-S-adenosyl-L-methionine complex are determined at 2.0 A resolution
crystals of the enzyme-S-adenosyl-L-methionine complex are grown from the reservoir solution containing 0.1 M Mes-NaOH, pH 6.0, and 14% PEG 10000. The crystals contain one PhTYW2 monomer with one AdoMet or MeSAdo molecule per asymmetric unit, and belong to the space group R3
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the enzyme is overexpressed in breast cancer
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D293A
mutant enzyme retains activity
E265A
mutation eliminates activity
K225A
mutation eliminates activity
T248A
mutant enzyme retains activity
Y242A
mutant enzyme retains activity
Show AA Sequence (236 entries)
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