Information on EC 2.5.1.113 - [CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase

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The expected taxonomic range for this enzyme is: Mycobacterium tuberculosis

EC NUMBER
COMMENTARY hide
2.5.1.113
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RECOMMENDED NAME
GeneOntology No.
[CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH = [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-cysteine biosynthesis V
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SYSTEMATIC NAME
IUBMB Comments
O-phospho-L-serine:thiocarboxylated [CysO sulfur-carrier protein] 2-amino-2-carboxyethyltransferase
A pyridoxal-phosphate protein. The enzyme participates in an alternative pathway for L-cysteine biosynthesis that involves a protein-bound thiocarboxylate as the sulfide donor. The enzyme from the bacterium Mycobacterium tuberculosis also has very low activity with O3-acetyl-L-serine (cf. EC 2.5.1.65, O-phosphoserine sulfhydrylase).
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
O-acetyl-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
?
show the reaction diagram
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the specificity of the enzyme for its amino acid substrate is more than 500fold greater for O-phospho-L-serine than for O-acetyl-L-serine
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?
O-acetyl-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + acetate
show the reaction diagram
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
O-acetyl-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + acetate
show the reaction diagram
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1
O-phospho-L-serine
Mycobacterium tuberculosis
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at pH 7.5 and 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.34 - 8.85
O-phospho-L-serine
1189
0.857 - 146
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
28992
PDB
SCOP
CATH
ORGANISM
UNIPROT
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme bound to CysO sulfur-carrier protein, hanging drop vapor diffusion method, using 7-10% PEG (w/v) 4000, 0.1 M sodium citrate, pH 5.8, and 0.2 M ammonium acetate at 22°C
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sitting drop vapor diffusion method, using 0.1 M Tris-HCl, pH 7.25-7.5, 0.1 M K2HPO4, 4.3 M NaCl
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA affinity column chromatography and Sephadex 200 gel filtration
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Ni-NTA column chromatography
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Ni-NTA column chromatography, Superdex-200 gel filtration, and Sephadex G-25 gel filtration
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nickel affinity column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli B834(DE3) and BL21(DE3) cells
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expressed in Escherichia coli BL21(DE3) cells
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
DELTA319-323
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the deletion mutant shows increased catalytic efficiency for O-phospho-L-serine and reduced catalytic efficiency for [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
R220A
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the mutant shows reduced activity with O-phospho-L-serine and increased activity with O-acetyl-L-serine compared to the wild type enzyme
DELTA319-323
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the deletion mutant shows increased catalytic efficiency for O-phospho-L-serine and reduced catalytic efficiency for [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
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R220A
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the mutant shows reduced activity with O-phospho-L-serine and increased activity with O-acetyl-L-serine compared to the wild type enzyme
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