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Information on EC 2.5.1.112 - adenylate dimethylallyltransferase (ADP/ATP-dependent) Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The enzyme appears in viruses and cellular organisms
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adenylate dimethylallyltransferase (ADP/ATP-dependent)
-
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dimethylallyl diphosphate + ADP = diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
(1)
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dimethylallyl diphosphate + ATP = diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
(2)
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trans-zeatin biosynthesis
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mevalonate metabolism
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Biosynthesis of secondary metabolites
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dimethylallyl-diphosphate:ADP/ATP dimethylallyltransferase
Involved in the biosynthesis of cytokinins in plants. The IPT4 isoform from the plant Arabidopsis thaliana is specific for ADP and ATP [1]. Other isoforms, such as IPT1 from Arabidopsis thaliana [1,2] and the enzyme from the common hop, Humulus lupulus [3], also have a lower activity with AMP (cf. EC 2.5.1.27, adenylate dimethylallyltransferase).
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2-isopentenyl-diphosphate:ADP/ATP DELTA2-isopentenyltransferase;
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adenylate isopentenyltransferase
ATP/ADP isopentenyltransferase
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cytokinin synthase
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ambiguous
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dimethylallyl diphosphate:ATP/ADP isopentenyltransferase
isopentenyl transferase 2
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isopentenyltransferase
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ambiguous
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adenylate isopentenyltransferase
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ambiguous
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adenylate isopentenyltransferase
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adenylate isopentenyltransferase
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adenylate isopentenyltransferase
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adenylate isopentenyltransferase
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adenylate isopentenyltransferase
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AIPT
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dimethylallyl diphosphate:ATP/ADP isopentenyltransferase
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dimethylallyl diphosphate:ATP/ADP isopentenyltransferase
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IPT
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ambiguous
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IPT4
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IPT5
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IPT7
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SwissProt
brenda
cultivar B73
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brenda
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brenda
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UniProt
brenda
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Uniprot
brenda
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UniProt
brenda
ecotype WS
UniProt
brenda
ecotype WS
Uniprot
brenda
ecotype WS
UniProt
brenda
isoform IPT5
UniProt
brenda
-
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brenda
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UniProt
brenda
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malfunction
the ipt1357 quadruple mutant possesses severely decreased levels of isopentenyladenine and trans-zeatin , and their corresponding ribosides, ribotides, and glucosides, and is retarded in its growth. In contrast, these mutants possess increased levels of cis-zeatin-type cytokinins; the ipt1357 quadruple mutant possesses severely decreased levels of isopentenyladenine and trans-zeatin , and their corresponding ribosides, ribotides, and glucosides, and is retarded in its growth. In contrast, these mutants possess increased levels of cis-zeatin-type cytokinins; the ipt1357 quadruple mutant possesses severely decreased levels of isopentenyladenine and trans-zeatin , and their corresponding ribosides, ribotides, and glucosides, and is retarded in its growth. In contrast, these mutants possess increased levels of cis-zeatin-type cytokinins; the ipt1357 quadruple mutant possesses severely decreased levels of isopentenyladenine and trans-zeatin , and their corresponding ribosides, ribotides, and glucosides, and is retarded in its growth. In contrast, these mutants possess increased levels of cis-zeatin-type cytokinins; the ipt1357 quadruple mutant possesses severely decreased levels of isopentenyladenine and trans-zeatin , and their corresponding ribosides, ribotides, and glucosides, and is retarded in its growth. In contrast, these mutants possess increased levels of cis-zeatin-type cytokinins; the ipt1357 quadruple mutant possesses severely decreased levels of isopentenyladenine and trans-zeatin , and their corresponding ribosides, ribotides, and glucosides, and is retarded in its growth. In contrast, these mutants possess increased levels of cis-zeatin-type cytokinins; the ipt1357 quadruple mutant possesses severely decreased levels of isopentenyladenine and trans-zeatin , and their corresponding ribosides, ribotides, and glucosides, and is retarded in its growth. In contrast, these mutants possess increased levels of cis-zeatin-type cytokinins
metabolism
the enzyme isoforms are responsible for the bulk of isopentenyladenine- and trans-zeatin-type cytokinin synthesis; the enzyme isoforms are responsible for the bulk of isopentenyladenine- and trans-zeatin-type cytokinin synthesis; the enzyme isoforms are responsible for the bulk of isopentenyladenine- and trans-zeatin-type cytokinin synthesis; the enzyme isoforms are responsible for the bulk of isopentenyladenine- and trans-zeatin-type cytokinin synthesis; the enzyme isoforms are responsible for the bulk of isopentenyladenine- and trans-zeatin-type cytokinin synthesis; the enzyme isoforms are responsible for the bulk of isopentenyladenine- and trans-zeatin-type cytokinin synthesis; the enzyme isoforms are responsible for the bulk of isopentenyladenine- and trans-zeatin-type cytokinin synthesis
metabolism
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the isopentenyltransferase reaction is the key rate-limiting step in cytokinin biosynthesis
physiological function
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the enzyme is expressed during kernel development
physiological function
interference of auxin distribution disrupts the cytokinin response and ATP/ADP isopentenyltransferase IPT5 expression, affecting stem cell initiation and meristem formation. Auxin response factor ARF3 mediates the auxin response during de novo organ regeneration. Mutations in ARF3 cause ectopic cytokinin biosynthesis via the misexpression of IPT5, and this disrupts organ regeneration. ARF3 directly binds to the promoter of IPT5 and negatively regulates IPT5 expression
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4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ADP
diphosphate + trans-zeatin riboside 5'-diphosphate
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?
4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ADP
trans-zeatin riboside phosphate + phosphate
2.2% activity compared to dimethylallyl diphosphate and ADP
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-
?
4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ATP
trans-zeatin riboside phosphate + diphosphate
1.4% activity compared to dimethylallyl diphosphate and ADP
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-
?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine phosphate
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
dimethylallyl diphosphate + CDP
?
30% activity compared to ADP
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-
?
dimethylallyl diphosphate + CTP
?
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?
dimethylallyl diphosphate + dADP
?
50% activity compared to ADP
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?
dimethylallyl diphosphate + dATP
?
dimethylallyl diphosphate + diadenosine hexaphosphate
?
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?
dimethylallyl diphosphate + diadenosine pentaphosphate
?
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diadenosine pentaphosphate exhibits the highest binding affinity
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?
dimethylallyl diphosphate + diadenosine tetraphosphate
?
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?
dimethylallyl diphosphate + diadenosine triphosphate
?
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diadenosine pentaphosphate exhibits the lowest binding affinity and specific activity compared to ATP
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?
dimethylallyl diphosphate + GDP
?
6.4% activity compared to ADP
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?
dimethylallyl diphosphate + GTP
?
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?
dimethylallyl diphosphate + UTP
?
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?
geranyl diphosphate + ADP
?
1.2% activity compared to dimethylallyl diphosphate and ADP
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?
isopentenyl diphosphate + ADP
?
0.3% activity compared to dimethylallyl diphosphate and ADP
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?
additional information
?
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dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
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?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
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?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
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?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
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?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
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?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
100% activity
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?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
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-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine phosphate
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low activity with AMP
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?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine phosphate
6.6% activity compared to ADP
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?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine phosphate
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low efficiency
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?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
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-
?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
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?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
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highest activity with ATP
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?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
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-
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?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
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ATP is the best cosubstrate
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?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
the enzme favors ATP as its ligand
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?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
82% activity compared to ADP
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?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
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?
dimethylallyl diphosphate + dATP
?
the enzyme shows preference for dATP over ADP and 80% activity with dATP compared to ATP
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?
dimethylallyl diphosphate + dATP
?
36% activity compared to ADP
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?
additional information
?
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no activity with AMP and tRNA
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additional information
?
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GMP, IMP, CMP, and UMP are not accepted as a substrate
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additional information
?
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no activity with NAD+, NADP+ or FAD
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additional information
?
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no activity with farnesyl diphosphate, dAMP, CMP, GMP, IMP, IDP, UMP, UDP, FAD and NADH
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additional information
?
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1-hydroxy-2-methyl-2-buten-4-yl 4-diphosphate is not a substrate
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4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ADP
diphosphate + trans-zeatin riboside 5'-diphosphate
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-
-
?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine phosphate
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
additional information
?
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1-hydroxy-2-methyl-2-buten-4-yl 4-diphosphate is not a substrate
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dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
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?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
Q93WC9, Q94ID1, Q94ID2, Q94ID3, Q9C6L1, Q9LJL4, Q9SB60
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?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
Q93WC9, Q94ID1, Q94ID2, Q94ID3, Q9C6L1, Q9LJL4, Q9SB60
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?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
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?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
Q5GHF7
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?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
Q08ET4
100% activity
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-
?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
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-
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?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine phosphate
Q08ET4
6.6% activity compared to ADP
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?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine phosphate
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low efficiency
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?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
Q93WC9, Q94ID1, Q94ID2, Q94ID3, Q9C6L1, Q9LJL4, Q9SB60
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?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
Q93WC9, Q94ID1, Q94ID2, Q94ID3, Q9C6L1, Q9LJL4, Q9SB60
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?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
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highest activity with ATP
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?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
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?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
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ATP is the best cosubstrate
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?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
Q5GHF7
the enzme favors ATP as its ligand
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?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
Q08ET4
82% activity compared to ADP
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?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
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?
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ADP
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preferred ligand over AMP
ADP
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the enzyme preferentially uses ADP and ATP over AMP
ATP
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preferred ligand over AMP
ATP
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the enzyme preferentially uses ADP and ATP over AMP
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Mg2+
the enzyme is dependent on presence of divalent cation, and addition of 2 mM MnCl2 is 1.3times more effective than that of 10 mM MgCl2
Mn2+
the enzyme is dependent on presence of divalent cation, and addition of 2 mM MnCl2 is 1.3times more effective than that of 10 mM MgCl2
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0.759
AMP
-
in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C
0.0065 - 0.0374
dimethylallyl diphosphate
0.0193
ADP
-
in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C
0.0193
ADP
in HEPES (20mM, pH 7.0; 150 mM NaCl, 3 mM dithiothreitol), at 25°C
0.0682
ADP
in 100 mM Tris-HCl buffer, pH 7.0, containing 50 mM KCl, 2 mM MnCl2, 1 mg/ml bovine serum albumin, and 1 mM dithiohtreitol, at 25°C
0.0162
ATP
-
in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C
0.0162
ATP
in HEPES (20mM, pH 7.0; 150 mM NaCl, 3 mM dithiothreitol), at 25°C
0.018
ATP
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in 25 mM Tris-HCl, pH 7.5, 10 mM MgCl2+, 5 mM 2-mercaptoethanol at 24°C
0.0065
dimethylallyl diphosphate
-
in 25 mM Tris-HCl, pH 7.5, 10 mM MgCl2+, 5 mM 2-mercaptoethanol at 24°C
0.0195
dimethylallyl diphosphate
-
in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C
0.0374
dimethylallyl diphosphate
in 100 mM Tris-HCl buffer, pH 7.0, containing 50 mM KCl, 2 mM MnCl2, 1 mg/ml bovine serum albumin, and 1 mM dithiohtreitol, at 25°C
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0.008
AMP
-
in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C
0.022
ATP
-
in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C
0.027 - 0.035
dimethylallyl diphosphate
0.024
ADP
-
in 100 mM TrisHCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C
0.024
ADP
in 100 mM Tris-HCl buffer, pH 7.0, containing 50 mM KCl, 2 mM MnCl2, 1 mg/ml bovine serum albumin, and 1 mM dithiohtreitol, at 25°C
0.027
dimethylallyl diphosphate
-
in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C
0.035
dimethylallyl diphosphate
in 100 mM Tris-HCl buffer, pH 7.0, containing 50 mM KCl, 2 mM MnCl2, 1 mg/ml bovine serum albumin, and 1 mM dithiohtreitol, at 25°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0109
AMP
-
in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C
1.34
ATP
-
in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C
0.921 - 1.397
dimethylallyl diphosphate
0.344
ADP
in 100 mM Tris-HCl buffer, pH 7.0, containing 50 mM KCl, 2 mM MnCl2, 1 mg/ml bovine serum albumin, and 1 mM dithiohtreitol, at 25°C
1.238
ADP
-
in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C
0.921
dimethylallyl diphosphate
in 100 mM Tris-HCl buffer, pH 7.0, containing 50 mM KCl, 2 mM MnCl2, 1 mg/ml bovine serum albumin, and 1 mM dithiohtreitol, at 25°C
1.397
dimethylallyl diphosphate
-
in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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brenda
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brenda
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brenda
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brenda
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brenda
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strong expression in developing kernel
brenda
expression of isoform IPT3
brenda
predominant expression of isoform IPT1
brenda
predominant expression of isoform IPT7
brenda
immature seed, predominant expression of isoform IPT1; immature seed, predominant expression of isoform IPT4; immature seed, predominant expression of isoform IPT8
brenda
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brenda
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brenda
predominant expression of isoform IPT4; predominant expression of isoform IPT8
brenda
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-
brenda
predominant expression of isoform IPT1; predominant expression of isoform IPT7
brenda
-
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brenda
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brenda
predominant expression of isoform IPT1; predominant expression of isoform IPT5; predominant expression of isoform IPT7
brenda
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brenda
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brenda
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brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
35810
x * 35810, calculated from amino acid sequence
36693
-
x * 36693, calculated from amino acid sequence
37000
-
x * 37000, SDS-PAGE
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?
-
x * 36693, calculated from amino acid sequence; x * 37000, SDS-PAGE
?
x * 35810, calculated from amino acid sequence
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
in complex with ATP, sitting drop vapor diffusion method, using 0.2 M ammonium tartrate and 20% (w/v) PEG 3350
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Ni-chelate affinity column chromatography
Ni-NTA agarose column chromatography
-
Ni-NTA resin column chromatography and Mono Q column chromatography
Talon metal affinity column chromatography
-
Ni-chelate affinity column chromatography
-
Ni-chelate affinity column chromatography
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expressed in Escherichia coli AD494(DE3)pLysS cells
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expressed in Escherichia coli BL21(DE3) cells
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expressed in Escherichia coli BL21(DE3)pLysS cells
expressed in Escherichia coli BL21-Star cells
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expressed in Escherichia coli M15 cells
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expressed in Escherichia coli M15[pREP4] cells
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auxin response factor ARF3 directly binds to the promoter of IPT5 and negatively regulates IPT5 expression
enzyme expression is developmentally regulated in kernel tissues and coincides with cytokinin accumulation. The expression gradually increases from 6 to 8 days after pollination (DAP), peaks at 8 DAP, and decreases gradually until 14 DAP before increasing again at later stages (15-34 DAP)
-
isoform IPT1 is down-regulated by the cytokinin 4-(indol-3-yl)butyric acid within 4 h; isoform IPT3 is down-regulated by the cytokinin 4-(indol-3-yl)butyric acid within 4 h; isoform IPT5 is down-regulated by the cytokinin 4-(indol-3-yl)butyric acid within 4 h; isoform IPT7 is down-regulated by the cytokinin 4-(indol-3-yl)butyric acid within 4 h
isoform IPT3 is rapidly induced by 1 h treatment with nitrate; isoform IPT5 is up-regulated by 4,4'-isopropylidenediphenol (bisphenol A) treatment for 4 h; isoform IPT7 is up-regulated by 4,4'-isopropylidenediphenol (bisphenol A) treatment for 4 h
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D49A
the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP
F120A
the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP
F157A
the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP
F93A
the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP
Q255A
the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP
W159A
the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP
Y153A
the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP
Y170A
the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP; the mutant retains 84% of wild type activity
Y217A
the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP
Y54A
the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP
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IPT1_ARATH
357
40765
Swiss-Prot
IPT3_ARATH
336
37915
Swiss-Prot
IPT4_ARATH
318
36674
Swiss-Prot
IPT5_ARATH
330
37395
Swiss-Prot
IPT6_ARATH
342
38363
Swiss-Prot
IPT7_ARATH
329
36980
Swiss-Prot
IPT8_ARATH
330
37322
Swiss-Prot
IPT_HUMLU
329
36605
Swiss-Prot
Q08ET4_MORAL
312
35813
TrEMBL
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Takei, K.; Dekishima, Y.; Eguchi, T.; Yamaya, T.; Sakakibara, H.
A new method for enzymatic preparation of isopentenyladenine-type and trans-zeatin-type cytokinins with radioisotope-labeling
J. Plant Res.
116
259-263
2003
Arabidopsis thaliana
brenda
Sakano, Y.; Okada, Y.; Matsunaga, A.; Suwama, T.; Kaneko, T.; Ito, K.; Noguchi, H.; Abe, I.
Molecular cloning, expression, and characterization of adenylate isopentenyltransferase from hop (Humulus lupulus L.)
Phytochemistry
65
2439-2446
2004
Humulus lupulus
brenda
Kakimoto, T.
Identification of plant cytokinin biosynthetic enzymes as dimethylallyl diphosphate:ATP/ADP isopentenyltransferases
Plant Cell Physiol.
42
677-685
2001
Arabidopsis thaliana
brenda
Miyawaki, K.; Matsumoto-Kitano, M.; Kakimoto, T.
Expression of cytokinin biosynthetic isopentenyltransferase genes in Arabidopsis: tissue specificity and regulation by auxin, cytokinin, and nitrate
Plant J.
37
128-138
2004
Arabidopsis thaliana (Q93WC9), Arabidopsis thaliana (Q94ID1), Arabidopsis thaliana (Q94ID2), Arabidopsis thaliana (Q94ID3), Arabidopsis thaliana (Q9C6L1), Arabidopsis thaliana (Q9LJL4), Arabidopsis thaliana (Q9SB60)
brenda
Abe, I.; Tanaka, H.; Abe, T.; Noguchi, H.
Enzymatic formation of unnatural cytokinin analogs by adenylate isopentenyltransferase from mulberry
Biochem. Biophys. Res. Commun.
355
795-800
2007
Morus alba (Q08ET4)
brenda
Miyawaki, K.; Tarkowski, P.; Matsumoto-Kitano, M.; Kato, T.; Sato, S.; Tarkowska, D.; Tabata, S.; Sandberg, G.; Kakimoto, T.
Roles of Arabidopsis ATP/ADP isopentenyltransferases and tRNA isopentenyltransferases in cytokinin biosynthesis
Proc. Natl. Acad. Sci. USA
103
16598-16603
2006
Arabidopsis thaliana, Arabidopsis thaliana (Q93WC9), Arabidopsis thaliana (Q94ID1), Arabidopsis thaliana (Q94ID2), Arabidopsis thaliana (Q94ID3), Arabidopsis thaliana (Q9C6L1), Arabidopsis thaliana (Q9LJL4), Arabidopsis thaliana (Q9SB60)
brenda
Brugiere, N.; Humbert, S.; Rizzo, N.; Bohn, J.; Habben, J.E.
A member of the maize isopentenyl transferase gene family, Zea mays isopentenyl transferase 2 (ZmIPT2), encodes a cytokinin biosynthetic enzyme expressed during kernel development. Cytokinin biosynthesis in maize
Plant Mol. Biol.
67
215-229
2008
Zea mays
brenda
Chu, H.M.; Ko, T.P.; Wang, A.H.
Crystal structure and substrate specificity of plant adenylate isopentenyltransferase from Humulus lupulus: distinctive binding affinity for purine and pyrimidine nucleotides
Nucleic Acids Res.
38
1738-1748
2010
Humulus lupulus (Q5GHF7)
brenda
Chu, H.M.; Chen, F.Y.; Ko, T.P.; Wang, A.H.
Binding and catalysis of Humulus lupulus adenylate isopentenyltransferase for the synthesis of isopentenylated diadenosine polyphosphates
FEBS Lett.
584
4083-4088
2010
Humulus lupulus
brenda
Cheng, Z.; Wang, L.; Sun, W.; Zhang, Y.; Zhou, C.; Su, Y.; Li, W.; Sun, T.; Zhao, X.; Li, X.; Cheng, Y.; Zhao, Y.; Xie, Q.; Zhang, X.
Pattern of auxin and cytokinin responses for shoot meristem induction results from the regulation of cytokinin biosynthesis by AUXIN RESPONSE FACTOR3
Plant Physiol.
161
240-251
2013
Arabidopsis thaliana (Q94ID2)
brenda
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