Information on EC 2.5.1.110 - 12alpha,13alpha-dihydroxyfumitremorgin C prenyltransferase

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The expected taxonomic range for this enzyme is: Aspergillus fumigatus

EC NUMBER
COMMENTARY hide
2.5.1.110
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RECOMMENDED NAME
GeneOntology No.
12alpha,13alpha-dihydroxyfumitremorgin C prenyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dimethylallyl diphosphate + 12alpha,13alpha-dihydroxyfumitremorgin C = diphosphate + fumitremorgin B
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
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fumitremorgin A biosynthesis
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Staurosporine biosynthesis
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fumitremorgin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
dimethylallyl-diphosphate:12alpha,13alpha-dihydroxyfumitremorgin C dimethylallyl-N-1-transferase
The enzyme from the fungus Aspergillus fumigatus also shows some activity with fumitremorgin C. Involved in the biosynthetic pathways of several indole alkaloids such as fumitremorgins and verruculogen.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dimethylallyl diphosphate + 12alpha,13alpha-dihydroxyfumitremorgin C
diphosphate + fumitremorgin B
show the reaction diagram
dimethylallyl diphosphate + fumitremorgin C
?
show the reaction diagram
relative activity of 10% compared to 12,13-dihydroxyfumitremorgin C
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?
additional information
?
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no activity with brevianamide F, tryprostatin A or B
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dimethylallyl diphosphate + 12alpha,13alpha-dihydroxyfumitremorgin C
diphosphate + fumitremorgin B
show the reaction diagram
B9WZX7
the enzyme is involved in the biosynthetic pathways of several indole alkaloids such as fumitremorgins and verruculogen
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-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
product formation is independent of the presence of metal ions. Even in the presence of EDTA no loss of enzyme activity is not detected
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.096
12alpha,13alpha-dihydroxyfumitremorgin C
37C, pH not specified in the publication
0.186
dimethylallyl diphosphate
37C, pH not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.03
12alpha,13alpha-dihydroxyfumitremorgin C
Aspergillus fumigatus
B9WZX7
37C, pH not specified in the publication
0.03
dimethylallyl diphosphate
Aspergillus fumigatus
B9WZX7
37C, pH not specified in the publication
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50100
2 * 50100, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
2 * 50100, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli and Saccharomyces cerevisiae
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expression in Saccharomyces cerevisiae