Information on EC 2.5.1.107 - verruculogen prenyltransferase

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The expected taxonomic range for this enzyme is: Aspergillus fischeri

EC NUMBER
COMMENTARY hide
2.5.1.107
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RECOMMENDED NAME
GeneOntology No.
verruculogen prenyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dimethylallyl diphosphate + verruculogen = diphosphate + fumitremorgin A
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
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fumitremorgin A biosynthesis
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Staurosporine biosynthesis
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fumitremorgin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
dimethylallyl-diphosphate:verruculogen dimethylallyl-O-transferase
Found in a number of fungi. Catalyses the last step in the biosynthetic pathway of the indole alkaloid fumitremorgin A. The enzyme from the fungus Neosartorya fischeri is also active with fumitremorgin B and 12alpha,13alpha-dihydroxyfumitremorgin C.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
putative
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dimethylallyl diphosphate + 12,13-dihydroxyfumitremorgin C
diphosphate + ?
show the reaction diagram
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?
dimethylallyl diphosphate + fumitremorgin B
diphosphate + ?
show the reaction diagram
enzyme catalyzes the regular O-prenylation of the hydroxy group at C13
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?
dimethylallyl diphosphate + verruculogen
diphosphate + fumitremorgin A
show the reaction diagram
enzyme catalyzes the regular O-prenylation of the hydroxy group at C13
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?
additional information
?
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.157
12,13-dihydroxyfumitremorgin C
pH 7.5, 37°C
0.0615
dimethylallyl diphosphate
pH 7.5, 37°C
0.032
fumitremorgin B
pH 7.5, 37°C
0.0057
verruculogen
pH 7.5, 37°C
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45000
5 * 52700, calculated for recombinant protein with His-tag, 5 * 45000, SDs-PAGE
52700
5 * 52700, calculated for recombinant protein with His-tag, 5 * 45000, SDs-PAGE
268000
gel filtration, recombinant protein with His-tag
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pentamer
5 * 52700, calculated for recombinant protein with His-tag, 5 * 45000, SDs-PAGE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli