Information on EC 2.4.99.B5 - acarviosyl transferase

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The expected taxonomic range for this enzyme is: Actinoplanes

EC NUMBER
COMMENTARY hide
2.4.99.B5
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
acarviosyl transferase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acarbose + nonyl alpha-D-glucopyranoside = acarviosyl-alpha-1,4-nonyl-D-glucopyranoside + maltose
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
acarbose:nonyl alpha-D-glucopyranoside alpha-(1->4)-acarviosyltransferase
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acarbose + cellobiose
acarviosyl-(1->4)-alpha-cellobiose + maltose
show the reaction diagram
acarbose + laminaribiose
acarviosyl-(1->4)-alpha-laminaribiose + maltose
show the reaction diagram
acarbose + maltoheptaose
acarviosyl-(1->4)-alpha-maltoheptaose + maltose
show the reaction diagram
acarbose + maltohexaose
acarviosyl-(1->4)-alpha-maltohexaose + maltose
show the reaction diagram
-
-
-
?
acarbose + maltopentaose
acarviosyl-(1->4)-alpha-maltopentaose + maltose
show the reaction diagram
-
-
-
?
acarbose + maltose
acarviosyl-alpha-(1->4)-maltose + maltose
show the reaction diagram
-
-
-
?
acarbose + maltotetraose
acarviosyl-(1->4)-alpha-maltotetraose + maltose
show the reaction diagram
-
-
-
?
acarbose + maltotriose
acarviosyl-alpha-(1-4)-maltotriose + maltose
show the reaction diagram
acarbose + maltotriose
acarviosyl-alpha-(1->4)-maltotriose + maltose
show the reaction diagram
acarbose + nonyl alpha-D-glucopyranoside
nonyl acarviosyl-(1->4)-alpha-D-glucopyranoside + maltose
show the reaction diagram
-
-
-
?
acarbose + xylobiose
acarviosyl-(1->4)-alpha-xylobiose + maltose
show the reaction diagram
-
-
-
?
acarviosyl glucose + maltose
acarviosyl-alpha-(1->4)-maltose + D-glucose
show the reaction diagram
-
-
-
?
acarviosyl-alpha-(1->4)-Glc-alpha-(1->4)-Glc-alpha-(1->4)-alpha-Glc + maltose
acarviosyl-maltose + Glc-alpha-(1->4)-Glc-alpha-(1->4)-Glc
show the reaction diagram
-
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acarbose + maltotriose
acarviosyl-alpha-(1-4)-maltotriose + maltose
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.65
acarbose
pH 6.3, 30°C
0.96
maltose
pH 6.3, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2
-
pH 6.5, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
isoelectric focusing, pH gradient from 3 to 10
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
76000
x * 76000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
thermostable up to 40°C
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
heterologous expression in Streptomyces lividans TK23
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D229N
-
mutant enzyme without acarviosyl transferase activity and 4-alpha-glucanotransferase activity
D328N
-
mutant enzyme without acarviosyl transferase activity and 4-alpha-glucanotransferase activity
E257Q
-
mutant enzyme without acarviosyl transferase activity and 4-alpha-glucanotransferase activity
G140H
-
mutation changes acarviosyl transferase into an enzyme with 4-alpha-glucanotransferase activity. This mutation introduces cyclodextrin-forming activity into acarviosyl transferase, converting 2% of starch into cyclodextrins; mutation decreases acarviosyl transferase activity, 4-alpha-glucanotransferase activity is strongly enhanced
G140H/Q327H
-
mutation changes acarviosyl transferase into an enzyme with 4-alpha-glucanotransferase activity. This mutation introduces cyclodextrin-forming activity into acarviosyl transferase, converting 1.2% of starch into cyclodextrins
G140H/Q327H/T329M
-
mutation changes acarviosyl transferase into an enzyme with 4-alpha-glucanotransferase activity. This mutation introduces cyclodextrin-forming activity into acarviosyl transferase, converting 7.4% of starch into cyclodextrins
Q327H
-
mutation decreases acarviosyl transferase activity, 4-alpha-glucanotransferase activity is reduced
T329M
-
mutation decreases acarviosyl transferase activity, 4-alpha-glucanotransferase activity is strongly enhanced