Information on EC 2.4.99.5 - galactosyldiacylglycerol alpha-2,3-sialyltransferase

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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
2.4.99.5
-
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RECOMMENDED NAME
GeneOntology No.
galactosyldiacylglycerol alpha-2,3-sialyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
CMP-N-acetyl-beta-neuraminate + 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol = CMP + 1,2-diacyl-3-[3-(N-acetyl-alpha-D-neuraminyl)-beta-D-galactosyl]-sn-glycerol
show the reaction diagram
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-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycosyl group transfer
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-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycerolipid metabolism
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-
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SYSTEMATIC NAME
IUBMB Comments
CMP-N-acetylneuraminate:1,2-diacyl-3-beta-D-galactosyl-sn-glycerol N-acetylneuraminyltransferase
The beta-D-galactosyl residue of the oligosaccharide of glycoproteins may also act as acceptor.
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CAS REGISTRY NUMBER
COMMENTARY hide
80237-98-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Swiss-Webster albino
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-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CMP-N-acetylneuraminate + 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol
CMP + 1,2-diacyl-3-[3-(alpha-D-N-acetylneuraminyl)-beta-D-galactosyl]-sn-glycerol
show the reaction diagram
-
i.e. galactosyldiacylglycerol, transfers N-acetylneuraminic acid to position C-3 of the galactosyl residue
i.e. sialosylgalactosyldiacylglycerol
?
CMP-N-acetylneuraminate + Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc
CMP + ?
show the reaction diagram
-
i.e. lacto-N-tetraose
-
-
?
CMP-N-acetylneuraminate + Galbeta1-3GlcNAcR
CMP + NeuAcalpha2-3Galbeta1-3GlcNAcR
show the reaction diagram
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i.e. rat asialo alpha1 acid glycoprotein
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-
?
CMP-N-acetylneuraminate + Galbeta1-4GlcNAc
CMP + NeuAcalpha2-3Galbeta1-4GlcNAc
show the reaction diagram
-
i.e. N-acetyllactosamine
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-
?
CMP-N-acetylneuraminate + Galbeta1-4GlcNAcR
CMP + NeuAcalpha2-3Galbeta1-4GlcNAcR
show the reaction diagram
-
i.e. human asialo alpha1 acid glycoprotein
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-
?
CMP-N-acetylneuraminic acid + 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol
CMP + 1,2-diacyl-3-[3-(alpha-D-N-acetyl-neuraminyl)-beta-D-galactosyl]-sn-glycerol
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminic acid + NeuAcalpha-(2-3)-Galbeta-(1-4)-GlcNAcbeta-1-octyl
?
show the reaction diagram
-
activity of the recombinant enzyme with sialylated beta-D-Gal-(1-3)-alpha-D-GalNAc-octyl containing oligosaccharides, synthesized in analogy to oligosaccharides found on mucin type complex O-glycans, overview
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-
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CMP-N-acetylneuraminate + 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol
CMP + 1,2-diacyl-3-[3-(alpha-D-N-acetylneuraminyl)-beta-D-galactosyl]-sn-glycerol
show the reaction diagram
-
i.e. galactosyldiacylglycerol, transfers N-acetylneuraminic acid to position C-3 of the galactosyl residue
i.e. sialosylgalactosyldiacylglycerol
?
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.057 - 0.78
CMP-acetylneuraminate
0.13
galactosyldiacylglycerol
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-
0.11
Galbeta1-3GlcNAcbeta1-1Galbeta1-4Glc
-
-
-
0.18
Galbeta1-3GlcNAcR
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-
0.18
Galbeta1-4GlcNaAcR
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-
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2.43
Galbeta1-4GlcNAc
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-
0.26
Galbeta1-4GlcNAcR
-
-
additional information
additional information
-
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
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use for chemoenzymatic synthesis of 13C-labeled sialyloligosaccharide
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LINKS TO OTHER DATABASES (specific for EC-Number 2.4.99.5)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)