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Information on EC 2.4.99.16 - starch synthase (maltosyl-transferring) Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The enzyme appears in viruses and cellular organisms
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starch synthase (maltosyl-transferring)
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alpha-maltose 1-phosphate + [(1->4)-alpha-D-glucosyl]n = phosphate + [(1->4)-alpha-D-glucosyl]n+2
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glycogen biosynthesis III (from alpha-maltose 1-phosphate)
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Starch and sucrose metabolism
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alpha-maltose 1-phosphate:(1->4)-alpha-D-glucan 4-alpha-D-maltosyltransferase
The enzyme from the bacterium Mycobacterium smegmatis is specific for maltose. It has no activity with alpha-D-glucose.
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alpha-maltose 1-phosphate:(1->4)-alpha-D-glucan 4-alpha-D-maltosyltransferase
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alpha1,4-glucan:maltose-1-P maltosyltransferase
alpha1,4-glucan:maltose-1-P maltosyltransferase
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alpha1,4-glucan:maltose-1-P maltosyltransferase
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alpha1,4-glucan:maltose-1-P maltosyltransferase
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GMPMT
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UniProt
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UniProt
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UniProt
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metabolism
the enzyme catalyzes the last step in the conversion of trehalose to glycogen transfering maltose from maltose 1-phosphate to glycogen. Trehalose synthase, maltokinase, and GMPMT represent an additional pathway of glycogen synthesis using trehalose as the source of glucose
metabolism
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the enzyme catalyzes the last step in the conversion of trehalose to glycogen transfering maltose from maltose 1-phosphate to glycogen. Trehalose synthase, maltokinase, and GMPMT represent an additional pathway of glycogen synthesis using trehalose as the source of glucose
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2-deoxy-2-fluoro-alpha-maltosyl fluoride + maltotetraose
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alpha-maltose 1-phosphate + [(1->4)-alpha-D-glucosyl]n
phosphate + [(1->4)-alpha-D-glucosyl]n+2
additional information
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alpha-maltose 1-phosphate + [(1->4)-alpha-D-glucosyl]n
phosphate + [(1->4)-alpha-D-glucosyl]n+2
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alpha-maltose 1-phosphate + [(1->4)-alpha-D-glucosyl]n
phosphate + [(1->4)-alpha-D-glucosyl]n+2
liver, oyster, or mycobacterial glycogens are the best acceptors, amylopectin has good activity, but amylose is a poor acceptor. GMPMT also appears to be able to catalyze arsenolysis of glycogen with release of malto-oligosaccharides or at least maltotriose
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alpha-maltose 1-phosphate + [(1->4)-alpha-D-glucosyl]n
phosphate + [(1->4)-alpha-D-glucosyl]n+2
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alpha-maltose 1-phosphate + [(1->4)-alpha-D-glucosyl]n
phosphate + [(1->4)-alpha-D-glucosyl]n+2
liver, oyster, or mycobacterial glycogens are the best acceptors, amylopectin has good activity, but amylose is a poor acceptor. GMPMT also appears to be able to catalyze arsenolysis of glycogen with release of malto-oligosaccharides or at least maltotriose
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alpha-maltose 1-phosphate + [(1->4)-alpha-D-glucosyl]n
phosphate + [(1->4)-alpha-D-glucosyl]n+2
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additional information
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GMPMT requires a high-molecular weight alpha-1,4-glucan as the acceptor. The enzyme can also transfer maltosyl units to maltosaccharides, e.g. maltotetraose to maltohexaose, overview
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additional information
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GMPMT requires a high-molecular weight alpha-1,4-glucan as the acceptor. The enzyme can also transfer maltosyl units to maltosaccharides, e.g. maltotetraose to maltohexaose, overview
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additional information
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GMPMT requires a high-molecular weight alpha-1,4-glucan as the acceptor. The enzyme can also transfer maltosyl units to maltosaccharides, e.g. maltotetraose to maltohexaose, overview
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alpha-maltose 1-phosphate + [(1->4)-alpha-D-glucosyl]n
phosphate + [(1->4)-alpha-D-glucosyl]n+2
alpha-maltose 1-phosphate + [(1->4)-alpha-D-glucosyl]n
phosphate + [(1->4)-alpha-D-glucosyl]n+2
Q9RP48
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alpha-maltose 1-phosphate + [(1->4)-alpha-D-glucosyl]n
phosphate + [(1->4)-alpha-D-glucosyl]n+2
Q9RP48
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alpha-maltose 1-phosphate + [(1->4)-alpha-D-glucosyl]n
phosphate + [(1->4)-alpha-D-glucosyl]n+2
Q9L1K2
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1,4-dideoxy-1,4-imino-D-arabinitol
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2-deoxy-2-fluoro-alpha-maltosyl fluoride
after preincubation for 5 min with 1 mM substrate analogue 2-deoxy-2-fluoro-alpha-maltosyl fluoride, 70% of the normal activity with alpha-maltose 1-phosphate is lost
D-glucose 1-phosphate
slight inhibition
D-glucose 6-phosphate
slight inhibition
diphosphate
slight inhibition
maltosaccharides
compete with glycogen for the transferred maltose
additional information
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no inhibition by isofagomine or acarbose
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additional information
no inhibition by isofagomine or acarbose
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additional information
additional information
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additional information
additional information
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kinetics, overview
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additional information
additional information
kinetics, overview
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0.76
purified enzyme, pH 7.0, 37°C
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Mycobacterium thermoresistibile (strain ATCC 19527 / DSM 44167 / CIP 105390 / JCM 6362 / NCTC 10409 / 316)
Mycobacterium thermoresistibile (strain ATCC 19527 / DSM 44167 / CIP 105390 / JCM 6362 / NCTC 10409 / 316)
Mycobacterium thermoresistibile (strain ATCC 19527 / DSM 44167 / CIP 105390 / JCM 6362 / NCTC 10409 / 316)
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
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homodimer
x-ray crystallography
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alpha-maltose 1-phosphate bound to a D394A mutant and beta-2-deoxy-2-fluoromaltosyl-enzyme intermediate with a E423A mutant is crystallized with 15% (w/v) polyethylene glycol 3350, 0.2 M sodium citrate, and 15% (w/v) ethylene glycol
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native enzyme GMPMT 89fold by ammonium sulfate fractionation, dialysis, anion exchange chromatography and gel filtration
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D394A
the maltosyl transferase activity of the mutant is more than 4 orders of magnitude lower than that of the wild type protein
E423A
the mutant turns over alpha-maltose 1-phosphate about 500times slower than the wild type enzyme
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Elbein, A.D.; Pastuszak, I.; Tackett, A.J.; Wilson, T.; Pan, Y.T.
Last step in the conversion of trehalose to glycogen: a mycobacterial enzyme that transfers maltose from maltose 1-phosphate to glycogen
J. Biol. Chem.
285
9803-9812
2010
Mycobacterium smegmatis, Mycobacterium smegmatis (Q9RP48), Mycobacterium smegmatis ATCC 14468 (Q9RP48)
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Syson, K.; Stevenson, C.E.; Rashid, A.M.; Saalbach, G.; Tang, M.; Tuukkanen, A.; Svergun, D.I.; Withers, S.G.; Lawson, D.M.; Bornemann, S.
Structural insight into how Streptomyces coelicolor maltosyl transferase GlgE binds alpha-maltose 1-phosphate and forms a maltosyl-enzyme intermediate
Biochemistry
53
2494-2504
2014
Streptomyces coelicolor (Q9L1K2), Streptomyces coelicolor
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