Information on EC 2.4.2.B2 - UDP-D-xylose:alpha-D-xyloside alpha1->3-xylosyltransferase

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The expected taxonomic range for this enzyme is: Homo sapiens

EC NUMBER
COMMENTARY hide
2.4.2.B2
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
UDP-D-xylose:alpha-D-xyloside alpha1->3-xylosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-D-xylose + Xylalpha(1-3)Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87) = UDP + Xyl-alpha(1-3)Xylalpha(1-3)Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
UDP-D-xylose:alpha-D-xyloside alpha-1,3-D-xylosyltransferase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-D-xylose + N-pyridin-2-yl-3-O-alpha-D-xylopyranosyl-beta-D-glucopyranosylamine
UDP + alpha-D-xylopyranosyl-(1-3)-alpha-D-xylopyranosyl-(1-3)-N-pyridin-2-yl-beta-D-glucopyranosylamine
show the reaction diagram
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the structure of the transfer product isolated is confirmed by Smith degradation, mass spectrometry, and a- and y9-xylosidase digestions
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?
additional information
?
-
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the enzyme participates in the biosynthesis of the Glc-O-Ser-type sugar chains
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
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the enzyme participates in the biosynthesis of the Glc-O-Ser-type sugar chains
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-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
restores activity after inhibition by EDTA
Mn2+
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restores activity after inhibition by EDTA
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
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activity is restored by addition of Mn2+ and Mg2
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
52
N-pyridin-2-yl-3-O-alpha-D-xylopyranosyl-beta-D-glucopyranosylamine
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pH 7.2, 37°C
0.28
UDP-D-xylose
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pH 7.2, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.6 - 7.6
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pH 6.6: about 60% of maximal activity, pH 7.6: about 40% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
230000
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gel filtration