Information on EC 2.4.2.51 - anthocyanidin 3-O-glucoside 2'''-O-xylosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.2.51
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RECOMMENDED NAME
GeneOntology No.
anthocyanidin 3-O-glucoside 2'''-O-xylosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-xylose + an anthocyanidin 3-O-beta-D-glucoside = UDP + an anthocyanidin 3-O-beta-D-sambubioside
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
anthocyanidin acylglucoside and acylsambubioside biosynthesis
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anthocyanidin modification (Arabidopsis)
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anthocyanidin sambubioside biosynthesis
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Anthocyanin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-xylose:anthocyanidin-3-O-beta-D-glucoside 2'''-O-xylosyltransferase
Isolated from the plants Matthiola incana (stock) [1] and Arabidopsis thaliana (mouse-eared cress) [2]. The enzyme has similar activity with the 3-glucosides of pelargonidin, cyanidin, delphinidin, quercetin and kaempferol as well as with cyanidin 3-O-rhamnosyl-(1->6)-glucoside and cyanidin 3-O-(6-acylglucoside). There is no activity with other UDP-sugars or with cyanidin 3,5-diglucoside.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
; gene ugt79b1
UniProt
Manually annotated by BRENDA team
; line 06
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
flavonoid UGT phylogenetic tree, UGT79B1 belongs to a cluster in which UGTs transfer a glycosyl group to a sugar moiety of flavonoid glycosides
malfunction
anthocyanin is drastically reduced in ugt79b1 knockout mutants
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-xylose + cyanidin 3-O-(6-O-(4-coumaroyl))-beta-D-glucoside
UDP + cyanidin 3-O-(6-O-(4-coumaroyl))-beta-D-sambubioside
show the reaction diagram
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-
-
-
?
UDP-alpha-D-xylose + cyanidin 3-O-(6-O-caffeoyl)-beta-D-glucoside
UDP + cyanidin 3-O-(6-O-caffeoyl)-beta-D-sambubioside
show the reaction diagram
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?
UDP-alpha-D-xylose + cyanidin 3-O-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside
UDP + cyanidin 3-O-beta-D-xylosyl-(1->2)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside
show the reaction diagram
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-
-
?
UDP-alpha-D-xylose + cyanidin 3-O-beta-D-glucoside
UDP + cyanidin 3-O-beta-D-sambubioside
show the reaction diagram
UDP-alpha-D-xylose + delphinidin 3-O-beta-D-glucoside
UDP + delphinidin 3-O-beta-D-sambubioside
show the reaction diagram
UDP-alpha-D-xylose + kaempferol 3-O-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside
UDP + kaempferol 3-O-beta-D-xylosyl-(1->2)-[alpha-L-rhamnosyl-(1->6)]-beta-D-glucoside
show the reaction diagram
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-
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?
UDP-alpha-D-xylose + kaempferol 3-O-beta-D-glucoside
UDP + kaempferol 3-O-beta-D-xylosyl-(1->2)-beta-D-glucoside
show the reaction diagram
best substrate
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-
?
UDP-alpha-D-xylose + pelargonidin 3-O-beta-D-glucoside
UDP + pelargonidin 3-O-beta-D-sambubioside
show the reaction diagram
UDP-alpha-D-xylose + quercetin 3-O-beta-D-glucoside
UDP + quercetin 3-O-beta-D-sambubioside
show the reaction diagram
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?
UDP-D-xylose + cyanidin 3-O-(alpha-L-rhamnosyl-(1->6)-beta-D-glucoside)
UDP + cyanidin 3-O-(beta-D-xylosyl-1(->2)[alpha-L-rhamnosyl-(1->6)]-beta-D-glucoside)
show the reaction diagram
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?
UDP-D-xylose + cyanidin 3-O-beta-D-glucoside
UDP + cyanidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
show the reaction diagram
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-
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?
UDP-D-xylose + delphinidin 3-O-beta-D-glucoside
UDP + delphinidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
show the reaction diagram
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?
UDP-D-xylose + kaempferol 3-O-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside
UDP + kaempferol 3-O-(beta-D-xylosyl-(1->2)[alpha-L-rhamnosyl-(1->6)]-beta-D-glucoside)
show the reaction diagram
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?
UDP-D-xylose + kaempferol 3-O-beta-D-glucoside
UDP + kaempferol 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
show the reaction diagram
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-
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?
UDP-D-xylose + pelargonidin 3-O-beta-D-glucoside
UDP + pelargonidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
show the reaction diagram
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?
UDP-D-xylose + quercetin 3-O-beta-D-glucoside
UDP + quercetin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
show the reaction diagram
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?
additional information
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-xylose + cyanidin 3-O-(6-O-(4-coumaroyl))-beta-D-glucoside
UDP + cyanidin 3-O-(6-O-(4-coumaroyl))-beta-D-sambubioside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-xylose + cyanidin 3-O-(6-O-caffeoyl)-beta-D-glucoside
UDP + cyanidin 3-O-(6-O-caffeoyl)-beta-D-sambubioside
show the reaction diagram
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-
-
-
?
UDP-alpha-D-xylose + cyanidin 3-O-beta-D-glucoside
UDP + cyanidin 3-O-beta-D-sambubioside
show the reaction diagram
UDP-alpha-D-xylose + delphinidin 3-O-beta-D-glucoside
UDP + delphinidin 3-O-beta-D-sambubioside
show the reaction diagram
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-
-
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?
UDP-alpha-D-xylose + pelargonidin 3-O-beta-D-glucoside
UDP + pelargonidin 3-O-beta-D-sambubioside
show the reaction diagram
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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activates 30% at 0.1 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CoCl2
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57% inhibition at 0.1 mM
CuSO4
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81% inhibition at 0.1 mM
diethyldicarbonate
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35% inhibition at 0.1 mM
diethyldithiocarbamate
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23% inhibition at 0.2 mM
EDTA
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38% inhibition at 0.2 mM
ethyleneglycolmonoethylether
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complete inhibition
FeSO4
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77% inhibition at 0.1 mM
KCN
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13% inhibition at 0.1 mM
MgCl2
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9% inhibition at 0.1 mM
MnCl2
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4% inhibition at 0.1 mM
ZnCl2
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64% inhibition at 0.1 mM
ZnSO4
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44% inhibition at 0.1 mM
additional information
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no effect by 4-chloromercuribenzoate at 0.02 mM
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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the enzyme activity is regulated during bud and flower development, overview
Manually annotated by BRENDA team
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the enzyme activity is regulated during bud and flower development, overview
Manually annotated by BRENDA team
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene ugt79b1, DNA and amino acid sequence determination and analysis, expression analysis and phylogenetic analysis, recombinant expression as His-tagged protein in Escherichia coli; recombinantly expressed as a His-tagged fusion protein
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information