Information on EC 2.4.2.49 - neamine phosphoribosyltransferase

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The expected taxonomic range for this enzyme is: Bacillus circulans

EC NUMBER
COMMENTARY hide
2.4.2.49
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RECOMMENDED NAME
GeneOntology No.
neamine phosphoribosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
neamine + 5-phospho-alpha-D-ribose 1-diphosphate = 5''-phosphoribostamycin + diphosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ribostamycin biosynthesis
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Neomycin, kanamycin and gentamicin biosynthesis
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Biosynthesis of antibiotics
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SYSTEMATIC NAME
IUBMB Comments
neamine:5-phospho-alpha-D-ribose 1-diphosphate phosphoribosyltransferase
Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including ribostamycin, neomycin and butirosin. The enzyme requires a divalent metal ion, optimally Mg2+, Ni2+ or Co2+.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
; gene btrL
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Manually annotated by BRENDA team
; gene btrL
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
neamine + 5-phospho-alpha-D-ribose 1-diphosphate
5''-phosphoribostamycin + diphosphate
show the reaction diagram
-
-
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
neamine + 5-phospho-alpha-D-ribose 1-diphosphate
5''-phosphoribostamycin + diphosphate
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
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activates
Mg2+
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activates
Ni2+
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activates
additional information
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BtrL requires essentially a divalent metal ion, optimally Mg2+, Ni2+, or Co2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
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high inhibition at 1 mM
EDTA
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complete inhibition at 1 mM
Zn2+
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high inhibition at 1 mM
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
the recombinant BtrL protein is largely unstable and loses activity within hours, addition of 10% glycerol in assay mixtures
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene btrL, soluble BtrL is only obtained by co-expression with the GroESL chaperones8 at low temperature in Escherichia coli BL21(DE3)
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