Information on EC 2.4.2.45 - decaprenyl-phosphate phosphoribosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.2.45
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RECOMMENDED NAME
GeneOntology No.
decaprenyl-phosphate phosphoribosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
trans,octacis-decaprenyl phosphate + 5-phospho-alpha-D-ribose 1-diphosphate = trans,octacis-decaprenylphospho-beta-D-ribofuranose 5-phosphate + diphosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
mycolyl-arabinogalactan-peptidoglycan complex biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
trans,octacis-decaprenylphospho-beta-D-ribofuranose 5-phosphate:diphosphate phospho-alpha-D-ribosyltransferase
Requires Mg2+. Isolated from Mycobacterium tuberculosis. Has some activity with other polyprenyl phosphates.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
no activity in Azorhizobium caulinodans
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
trans,octacis-decaprenyl phosphate + 5-phospho-alpha-D-ribose 1-diphosphate
trans,octacis-decaprenylphospho-beta-D-ribofuranose 5-phosphate + diphosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
trans,octacis-decaprenyl phosphate + 5-phospho-alpha-D-ribose 1-diphosphate
trans,octacis-decaprenylphospho-beta-D-ribofuranose 5-phosphate + diphosphate
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
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x * 30000, SDS-PAGE
33000
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x * 33000, estimated from amino acid sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 30000, SDS-PAGE; x * 33000, estimated from amino acid sequence
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli ER2566 cells
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A35E
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the mutant is associated with high-level ethambutol resistance and increases decaprenylphosphoryl-beta-D-arabinose levels
D77E
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15% activity compared to the wild type enzyme
E195A
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17% activity compared to the wild type enzyme
F176L
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the mutation is associated with ethambutol resistance
F59A
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94% activity compared to the wild type enzyme
F62A
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72% activity compared to the wild type enzyme
G165C
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the mutant is associated with high-level ethambutol resistance and increases decaprenylphosphoryl-beta-D-arabinose levels
H84L
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15% activity compared to the wild type enzyme
K174T
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the mutation is associated with ethambutol resistance
N29A
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18% activity compared to the wild type enzyme
N73Q
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15% activity compared to the wild type enzyme
R201A
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1% activity compared to the wild type enzyme
R22L
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10% activity compared to the wild type enzyme
W175C
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the mutation is associated with ethambutol resistance
F176L
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the mutation is associated with ethambutol resistance
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K174T
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the mutation is associated with ethambutol resistance
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W175C
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the mutation is associated with ethambutol resistance
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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the enzyme is a good target for the development of tuberculosis drugs
Show AA Sequence (112 entries)
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