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Enzyme Nomenclature
Information on EC 2.4.2.39 - xyloglucan 6-xylosyltransferase
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The enzyme appears in viruses and cellular organisms
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EC NUMBER 
COMMENTARY 
2.4.2.39
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RECOMMENDED NAME
GeneOntology No.
xyloglucan 6-xylosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Transfers an alpha-D-xylosyl residue from UDP-D-xylose to a glucose residue in xyloglucan, forming an alpha-(1->6)-D-xylosyl-D-glucose linkage
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
hexosyl group transfer
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-
-
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hexosyl group transfer
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xyloglucan alpha-(1,6)-xylosyltransferase activity, cell wall xyloglucan biosynthesis
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
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SYSTEMATIC NAME
IUBMB Comments
UDP-D-xylose:xyloglucan 6-alpha-D-xylosyltransferase
In association with EC 2.4.1.168 (xyloglucan 4-glucosyltransferase), this enzyme brings about the synthesis of xyloglucan; concurrent transfers of glucose and xylose are necessary for this synthesis.
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CAS REGISTRY NUMBER
COMMENTARY
80238-01-3
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
double mutants for either xxt2 or xxt5 have a large impact on XyG content, structure and size distribution; double mutants for either xxt2 or xxt5 have a large impact on XyG content, structure and size distribution; overexpression of XXT3, is able to restore XyG epitopes in xxt2, xxt5 and xxt2 xxt5 double knockouts, suggesting that it also encodes a protein with XXT activity
physiological function
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the enzyme maintains cell wall structure and tensile strength in rice
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
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-
-
?
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
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reduction of xyloglucan content (reduced glucan backbone substitution with xylose) in plants that lack functional XXT5 can be complemented by transformation with recombinant XXT5
-
?
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
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xylosyl-transfer is closely linked to glucosyl-transfer (EC 2.4.1.168)
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?
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
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no acceptors are cello-oligosaccharides and fragment oligosaccharides from endoglucanase digest
forms an alpha-1,6-D-xylosyl-D-glucose linkage
?
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
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other xylosyl-acceptors are beta-1,3-glucan and xylan
forms an alpha-1,6-D-xylosyl-D-glucose linkage
?
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
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transfers an alpha-D-xylosyl residue from UDP-D-xylose to a glucose residue in xyloglucan
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?
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
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transfers an alpha-D-xylosyl residue from UDP-D-xylose to a glucose residue in xyloglucan
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?
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
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transfers an alpha-D-xylosyl residue from UDP-D-xylose to a glucose residue in xyloglucan
forms an alpha-1,6-D-xylosyl-D-glucose linkage
?
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
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-
-
-
?
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
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GDP-D-glucose or GDP-D-mannose cannot replace UDPxylose
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?
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
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transfers an alpha-D-xylosyl residue from UDP-D-xylose to a glucose residue in xyloglucan
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?
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
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standard oligosaccharides for enzyme assay
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-
?
UDP-D-xylose + xyloglucan
?
-
responsible for xyloglucan side-chain formation
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-
?
UDP-D-xylose + xyloglucan
?
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involved in xyloglucan biosynthesis of higher plants
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-
?
additional information
?
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no xylosyltransferase activity in vitro after expression in either Pichia pastoris or Sf21 insect cells using UDP-xylose and a broad range of putative acceptors
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additional information
?
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no xylosyltransferase activity in vitro after expression in either Pichia pastoris or Sf21 insect cells using UDP-xylose and a broad range of putative acceptors
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
Q9CA75
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reduction of xyloglucan content (reduced glucan backbone substitution with xylose) in plants that lack functional XXT5 can be complemented by transformation with recombinant XXT5
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?
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
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-
?
UDP-D-xylose + xyloglucan
?
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responsible for xyloglucan side-chain formation
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-
?
UDP-D-xylose + xyloglucan
?
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involved in xyloglucan biosynthesis of higher plants
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-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
Mn2+
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
TDPglucose
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activation, as effective as UDPglucose, in concentrations exceeding UDPxylose
GDP-D-glucose
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about half as effective as UDPglucose, in concentrations exceeding UDPxylose; activation
UDPglucose
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activation, xylose is effectively incorporated in the presence of UDPglucose, the transfer must be preceded by elongation of the beta-1,4-glucan-backbone, because xylosyl residues constitute the side chains, in concentrations exceeding UDPxylose, no activation by CDPglucose or ADPglucose
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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high expression level, revealed by Reverse Transcription-PCR
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expressed in all major organs, revealed by Reverse Transcription-PCR
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high expression level, revealed by Reverse Transcription-PCR, shorter root hair and less root tissue xyloglucan detectable upon lack of XXT5
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silique, lowest expression level, revealed by Reverse Transcription-PCR
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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immunolocalization of recombinant enzyme using tag-specific antibody, predicted Golgi-resident type-II membrane protein
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complex formation in vivo and potential physical interactions among three xylosyltransferases, XXT1, XXT2, and XXT5, and a glucan synthase, CSLC4 is shown in the Golgi membrane in Arabidopsis cells; complex formation in vivo and potential physical interactions among three xylosyltransferases, XXT1, XXT2, and XXT5, and a glucan synthase, CSLC4 is shown in the Golgi membrane in Arabidopsis cells; complex formation in vivo and potential physical interactions among three xylosyltransferases, XXT1, XXT2, and XXT5, and a glucan synthase, CSLC4 is shown in the Golgi membrane in Arabidopsis cells
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
heterodimer
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existence of heterocomplexes XXT2-XXT5 and XXT1-XXT2 are demonstrated; existence of heterocomplexes XXT2-XXT5 and XXT5-CSLC4 are demonstrated; existence of heterocomplex XXT1-XXT2 is demonstrated
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as a truncated protein lacking the N-terminal transmembrane domain as GST-fusion protein; expressed in Escherichia coli as a truncated protein lacking the N-terminal transmembrane domain as GST-fusion protein; expressed in Escherichia coli as a truncated protein lacking the N-terminal transmembrane domain as GST-fusion protein; expressed in Escherichia coli as a truncated protein lacking the N-terminal transmembrane domain as GST-fusion protein
in pENTR/D-TOPO downstream of cauliflower mosaic virus 35S promoter for recombination with pEarleyGate 201 and Agrobacterium tumefacies strain GV3101-mediated transformation (floral-flip), expression with hemagglutinin (HA) tag
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LINKS TO OTHER DATABASES (specific for EC-Number 2.4.2.39)
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