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Information on EC 2.4.2.27 - dTDP-dihydrostreptose-streptidine-6-phosphate dihydrostreptosyltransferase Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The expected taxonomic range for this enzyme is: Streptomyces griseus
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dTDP-dihydrostreptose-streptidine-6-phosphate dihydrostreptosyltransferase
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dTDP-L-dihydrostreptose + streptidine 6-phosphate = dTDP + O-(1->4)-alpha-L-dihydrostreptosyl-streptidine 6-phosphate
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pentosyl group transfer
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streptomycin biosynthesis
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Streptomycin biosynthesis
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Biosynthesis of antibiotics
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dTDP-L-dihydrostreptose:streptidine-6-phosphate dihydrostreptosyltransferase
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dihydrostreptosyltransferase, thymidine diphosphodihydrostreptose-streptidine 6-phosphate
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dTDP-L-dihydrostreptose + streptidine 6-phosphate
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biosynthesis of streptomycin
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dTDP-L-dihydrostreptose + streptidine 6-phosphate
dTDP + O-alpha-L-dihydrostreptosyl-1,4-streptidine 6-phosphate
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additional information
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not: streptidine, 2-deoxystreptamine, 4-deoxystreptamine
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dTDP-L-dihydrostreptose + streptidine 6-phosphate
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biosynthesis of streptomycin
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Co2+
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as effective as Mg2+
Mg2+
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Mn2+ or Mg2+ required, maximal activity with 10 mM Mg2+
Mn2+
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Mn2+ or Mg2+ required, maximal activity with 3 mM Mn2+
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additional information
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8.2
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glycine/NaOH buffer
additional information
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no distinct pH-optimum observed in Tris-HCl buffer between pH 7.3 and 9
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35000
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2 * 35000, SDS-PAGE in presence of 2-mercaptoethanol
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dimer
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2 * 35000, SDS-PAGE in presence of 2-mercaptoethanol
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addition of streptidine is important for stabilization of enzyme activity during purification, purified transferase is also stable in absence of streptidine
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4°C, 50 mM Tris-HCl, pH 7.8 at 0°C, containing 5 mM mercaptoethanol, 1 mM EDTA, 2.5 mM streptidine, 10% v/v glycerol, stable for at least 4 months
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ammonium sulfate precipitation, Sephadex G-200, chromatography on DEAE-cellulose, Ultrogel AcA44 column, DEAE-Sepharose column
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STRH_STRGR
384
42900
Swiss-Prot
G0Q8I4_9ACTN
384
42916
TrEMBL
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Kniep, B.; Grisebach, H.
Biosynthesis of streptomycin. Purification and properties of a dTDP-L-dihydrostreptose:streptidine-6-phosphate dihydrostreptosyltransferase from Streptomyces griseus
Eur. J. Biochem.
105
139-144
1980
Streptomyces griseus
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