Information on EC 2.4.1.B70 - cyanidin 3-O-[2-O-(2-O-(sinapoyl)-beta-D-xylosyl) 6-O-(p-coumaroyl)-beta-D-glucoside] 5-O-[6-O-(malonyl)-beta-D-glucoside] 4-O-beta-D-glucosyltransferase (acyl-glucose)

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The expected taxonomic range for this enzyme is: Arabidopsis thaliana

EC NUMBER
COMMENTARY hide
2.4.1.B70
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RECOMMENDED NAME
GeneOntology No.
cyanidin 3-O-[2-O-(2-O-(sinapoyl)-beta-D-xylosyl) 6-O-(p-coumaroyl)-beta-D-glucoside] 5-O-[6-O-(malonyl)-beta-D-glucoside] 4-O-beta-D-glucosyltransferase (acyl-glucose)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1-O-(4-coumaroyl)-beta-D-glucose + cyanidin 3-O-[2-O-(2-O-(sinapoyl)-beta-D-xylosyl)-6-O-(6-(4-coumaroyl))-beta-D-glucoside] 5-O-[6-O-(malonyl)-beta-D-glucoside] = 4-coumarate + cyanidin 3-O-[2-O-(2-O-(sinapoyl)-beta-D-xylosyl)-6-O-(6-(4-O-beta-D-glucosyl)-4-coumaroyl)-beta-D-glucoside] 5-O-[6-O-(malonyl)-beta-D-glucoside]
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
hydroxycinnamoyl-alpha-D-glucose:cyanidin 3-O-[2-O-(2-O-(sinapoyl)-beta-D-xylosyl) 6-O-(p-coumaroyl)-beta-D-glucoside] 5-O-[6-O-(malonyl)-beta-D-glucoside] 4-O-glucosyltransferase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cf. EC 3.2.1.21
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
BGLU10 KO lines accumulate substrate cyanidin 3-O-[2-O-(2-O-(sinapoyl)xylosyl) 6-O-(p-coumaroyl) glucoside] 5-O-[6-O-(malonyl) glucoside]. The expression pattern of BGLU10 and the increase in acyl-glucose-dependent anthocyanin glucosyltransferase activity are consistent with the ratio of accumulating A11 to A9 anthocyanin
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-O-(4-coumaroyl)-beta-D-glucose + cyanidin 3-O-[2-O-(2-O-(sinapoyl)-beta-D-xylosyl)-6-O-(6-(4-coumaroyl))-beta-D-glucoside] 5-O-[6-O-(malonyl)-beta-D-glucoside]
4-coumarate + cyanidin 3-O-[2-O-(2-O-(sinapoyl)-beta-D-xylosyl)-6-O-(6-(4-O-beta-D-glucosyl)-4-coumaroyl)-beta-D-glucoside] 5-O-[6-O-(malonyl)-beta-D-glucoside]
show the reaction diagram
i.e. A9 anthocyanin molecule
i.e. A11 anthocyanin molecule
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?
additional information
?
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glucosylation occurs at the 4-coumarate moiety of the anthocyanin molecule cyanidin 3-O-[2-O-(2-O-(sinapoyl)xylosyl) 6-O-(p-coumaroyl) glucoside] 5-O-[6-O-(malonyl) glucoside] leading to completion of the main anthocyanin structure. Enzyme is more efficient with hydroxycinnamoyl-glucoses (C6-C3-glucoses) than benzoyl-glucose derivatives
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1-O-(4-coumaroyl)-beta-D-glucose + cyanidin 3-O-[2-O-(2-O-(sinapoyl)-beta-D-xylosyl)-6-O-(6-(4-coumaroyl))-beta-D-glucoside] 5-O-[6-O-(malonyl)-beta-D-glucoside]
4-coumarate + cyanidin 3-O-[2-O-(2-O-(sinapoyl)-beta-D-xylosyl)-6-O-(6-(4-O-beta-D-glucosyl)-4-coumaroyl)-beta-D-glucoside] 5-O-[6-O-(malonyl)-beta-D-glucoside]
show the reaction diagram
Q93ZI4
i.e. A9 anthocyanin molecule
i.e. A11 anthocyanin molecule
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?
additional information
?
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Q93ZI4
glucosylation occurs at the 4-coumarate moiety of the anthocyanin molecule cyanidin 3-O-[2-O-(2-O-(sinapoyl)xylosyl) 6-O-(p-coumaroyl) glucoside] 5-O-[6-O-(malonyl) glucoside] leading to completion of the main anthocyanin structure. Enzyme is more efficient with hydroxycinnamoyl-glucoses (C6-C3-glucoses) than benzoyl-glucose derivatives
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
highest levels of BGLU10 expression are observed on day 4 after anthocyanin induction