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Enzyme Nomenclature
Information on EC 2.4.1.B4 - sterol 27-beta-glucosyltransferase
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The expected taxonomic range for this enzyme is: Withania somnifera
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EC NUMBER 
COMMENTARY 
2.4.1.B4
preliminary BRENDA-supplied EC number
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RECOMMENDED NAME
GeneOntology No.
sterol 27-beta-glucosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + a 27-hydroxysterol = UDP + a sterol 27-beta-D-glucoside
enzyme transferes glucose to beta-OH present at C-17, C-21 and C-27 positions in sterols/withanolides, does not glycosylate sterols at the C-3 position
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UDP-glucose + a 27-hydroxysterol = UDP + a sterol 27-beta-D-glucoside
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-
-
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:sterol 27-O-beta-D-glucosyltransferase
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-glucose + 17alpha-hydroxy withaferin A
UDP + ?
-
15% of the activity with testosterone as substrate
-
-
?
UDP-glucose + 21-hydroxyprogesterone
UDP + ?
-
13% of the activity with testosterone as substrate
-
-
?
UDP-glucose + 27-hydroxy withanone
UDP + ?
-
15% of the activity with testosterone as substrate
-
-
?
UDP-glucose + 5alpha,6beta,17alpha,27-tetrahydroxywithanolide
UDP + ?
-
23% of the activity with testosterone as substrate
-
-
?
UDP-glucose + estradiol
UDP + ?
-
41% of the activity with testosterone as substrate
-
-
?
UDP-glucose + hydrocortisone
UDP + ?
-
11% of the activity with testosterone as substrate
-
-
?
UDP-glucose + testosterone
UDP + testosterone-17-yl beta-D-glucopyranoside
-
100% of activity
-
-
?
UDP-glucose + withanolide A
UDP + ?
-
21% of the activity with testosterone as substrate
-
-
?
UDP-glucose + withanolide U
UDP + ?
-
17% of the activity with testosterone as substrate
-
-
?
additional information
?
-
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can conjugate glucose to C-27 position in the withanolide lactone ring and to higher positions in sterols using UDP-glucose as glucose donor, activity is enhanced following the application of salicylic acid and decreased rapidly on exposure of the plants to heat shock, suggesting a functional role of the enzyme in biotic and abiotic stresses
-
-
-
additional information
?
-
-
UDP-glucose can not be replaced by UDP-galactose
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-
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
can conjugate glucose to C-27 position in the withanolide lactone ring and to higher positions in sterols using UDP-glucose as glucose donor, activity is enhanced following the application of salicylic acid and decreased rapidly on exposure of the plants to heat shock, suggesting a functional role of the enzyme in biotic and abiotic stresses
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-
-
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.01
(5alpha, 6beta,17alpha)-5,6,17,27-tetrahydroxywithanolide
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pH 8.0, 30°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 2.4.1.B4)
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