Information on EC 2.4.1.B4 - sterol 27-beta-glucosyltransferase

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The expected taxonomic range for this enzyme is: Withania somnifera

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EC NUMBER
COMMENTARY hide
2.4.1.B4
preliminary BRENDA-supplied EC number
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RECOMMENDED NAME
GeneOntology No.
sterol 27-beta-glucosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + a 27-hydroxysterol = UDP + a sterol 27-beta-D-glucoside
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:sterol 27-O-beta-D-glucosyltransferase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-glucose + 17alpha-hydroxy withaferin A
UDP + ?
show the reaction diagram
-
15% of the activity with testosterone as substrate
-
-
?
UDP-glucose + 21-hydroxyprogesterone
UDP + ?
show the reaction diagram
-
13% of the activity with testosterone as substrate
-
-
?
UDP-glucose + 27-hydroxy withanone
UDP + ?
show the reaction diagram
-
15% of the activity with testosterone as substrate
-
-
?
UDP-glucose + 5alpha,6beta,17alpha,27-tetrahydroxywithanolide
UDP + ?
show the reaction diagram
-
23% of the activity with testosterone as substrate
-
-
?
UDP-glucose + estradiol
UDP + ?
show the reaction diagram
-
41% of the activity with testosterone as substrate
-
-
?
UDP-glucose + hydrocortisone
UDP + ?
show the reaction diagram
-
11% of the activity with testosterone as substrate
-
-
?
UDP-glucose + testosterone
UDP + testosterone-17-yl beta-D-glucopyranoside
show the reaction diagram
-
100% of activity
-
-
?
UDP-glucose + withanolide A
UDP + ?
show the reaction diagram
-
21% of the activity with testosterone as substrate
-
-
?
UDP-glucose + withanolide U
UDP + ?
show the reaction diagram
-
17% of the activity with testosterone as substrate
-
-
?
additional information
?
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
can conjugate glucose to C-27 position in the withanolide lactone ring and to higher positions in sterols using UDP-glucose as glucose donor, activity is enhanced following the application of salicylic acid and decreased rapidly on exposure of the plants to heat shock, suggesting a functional role of the enzyme in biotic and abiotic stresses
-
-
-
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Triton X-100
-
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
(5alpha, 6beta,17alpha)-5,6,17,27-tetrahydroxywithanolide
-
pH 8.0, 30°C
0.17
17alpha-hydroxy withaferin
-
pH 8.0, 30°C
0.07
21beta-hydroxy progesterone
-
pH 8.0, 30°C
0.11
27-hydroxy withanone
-
pH 8.0, 30°C
0.04
estradiol
-
pH 8.0, 30°C
0.17
testosterone
-
pH 8.0, 30°C
0.02
UDP-glucose
-
pH 8.0, 30°C
0.11
withanolide A
-
pH 8.0, 30°C
0.05
withanolide U
-
pH 8.0, 30°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9
-
half maximum activity at pH 7 and 9
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.8
-
chromatofocusing
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
52000
-
gel filtration, SDS-PAGE
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 52000, SDS-PAGE, native mass by gel filtration
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LINKS TO OTHER DATABASES (specific for EC-Number 2.4.1.B4)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)