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Information on EC 2.4.1.B4 - sterol 27-beta-glucosyltransferase Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The expected taxonomic range for this enzyme is: Withania somnifera
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2.4.1.B4
preliminary BRENDA-supplied EC number
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sterol 27-beta-glucosyltransferase
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UDP-glucose + a 27-hydroxysterol = UDP + a sterol 27-beta-D-glucoside
UDP-glucose + a 27-hydroxysterol = UDP + a sterol 27-beta-D-glucoside
enzyme transferes glucose to beta-OH present at C-17, C-21 and C-27 positions in sterols/withanolides, does not glycosylate sterols at the C-3 position
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UDP-glucose + a 27-hydroxysterol = UDP + a sterol 27-beta-D-glucoside
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UDP-glucose:sterol 27-O-beta-D-glucosyltransferase
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additional information
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distinct from EC 2.4.1.173, does not glycosylate sterols at the C-3 position
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brenda
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UDP-glucose + 17alpha-hydroxy withaferin A
UDP + ?
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15% of the activity with testosterone as substrate
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-
?
UDP-glucose + 21-hydroxyprogesterone
UDP + ?
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13% of the activity with testosterone as substrate
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-
?
UDP-glucose + 27-hydroxy withanone
UDP + ?
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15% of the activity with testosterone as substrate
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?
UDP-glucose + 5alpha,6beta,17alpha,27-tetrahydroxywithanolide
UDP + ?
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23% of the activity with testosterone as substrate
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?
UDP-glucose + estradiol
UDP + ?
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41% of the activity with testosterone as substrate
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?
UDP-glucose + hydrocortisone
UDP + ?
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11% of the activity with testosterone as substrate
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?
UDP-glucose + testosterone
UDP + testosterone-17-yl beta-D-glucopyranoside
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100% of activity
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?
UDP-glucose + withanolide A
UDP + ?
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21% of the activity with testosterone as substrate
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?
UDP-glucose + withanolide U
UDP + ?
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17% of the activity with testosterone as substrate
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?
additional information
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additional information
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can conjugate glucose to C-27 position in the withanolide lactone ring and to higher positions in sterols using UDP-glucose as glucose donor, activity is enhanced following the application of salicylic acid and decreased rapidly on exposure of the plants to heat shock, suggesting a functional role of the enzyme in biotic and abiotic stresses
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additional information
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UDP-glucose can not be replaced by UDP-galactose
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additional information
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can conjugate glucose to C-27 position in the withanolide lactone ring and to higher positions in sterols using UDP-glucose as glucose donor, activity is enhanced following the application of salicylic acid and decreased rapidly on exposure of the plants to heat shock, suggesting a functional role of the enzyme in biotic and abiotic stresses
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0.01
(5alpha, 6beta,17alpha)-5,6,17,27-tetrahydroxywithanolide
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pH 8.0, 30°C
0.17
17alpha-hydroxy withaferin
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pH 8.0, 30°C
0.07
21beta-hydroxy progesterone
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pH 8.0, 30°C
0.11
27-hydroxy withanone
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pH 8.0, 30°C
0.04
estradiol
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pH 8.0, 30°C
0.17
testosterone
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pH 8.0, 30°C
0.02
UDP-glucose
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pH 8.0, 30°C
0.11
withanolide A
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pH 8.0, 30°C
0.05
withanolide U
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pH 8.0, 30°C
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7 - 9
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half maximum activity at pH 7 and 9
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brenda
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brenda
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52000
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gel filtration, SDS-PAGE
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monomer
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1 * 52000, SDS-PAGE, native mass by gel filtration
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Madina, B.R.; Sharma, L.K.; Chaturvedi, P.; Sangwan, R.S.; Tuli, R.
Purification and characterization of a novel glucosyltransferase specific to 27beta-hydroxy steroidal lactones from Withania somnifera and its role in stress responses
Biochim. Biophys. Acta
1774
1199-1207
2007
Withania somnifera
brenda
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