HOME
login
history
all enzymes
BRENDA home
History
Enzyme Nomenclature
Information on EC 2.4.1.B4 - sterol 27-beta-glucosyltransferase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
The expected taxonomic range for this enzyme is: Withania somnifera
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
EC NUMBER 
COMMENTARY 
2.4.1.B4
preliminary BRENDA-supplied EC number
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
RECOMMENDED NAME
GeneOntology No.
sterol 27-beta-glucosyltransferase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + a 27-hydroxysterol = UDP + a sterol 27-beta-D-glucoside
enzyme transferes glucose to beta-OH present at C-17, C-21 and C-27 positions in sterols/withanolides, does not glycosylate sterols at the C-3 position
-
UDP-glucose + a 27-hydroxysterol = UDP + a sterol 27-beta-D-glucoside
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:sterol 27-O-beta-D-glucosyltransferase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-glucose + 17alpha-hydroxy withaferin A
UDP + ?
-
15% of the activity with testosterone as substrate
-
-
?
UDP-glucose + 21-hydroxyprogesterone
UDP + ?
-
13% of the activity with testosterone as substrate
-
-
?
UDP-glucose + 27-hydroxy withanone
UDP + ?
-
15% of the activity with testosterone as substrate
-
-
?
UDP-glucose + 5alpha,6beta,17alpha,27-tetrahydroxywithanolide
UDP + ?
-
23% of the activity with testosterone as substrate
-
-
?
UDP-glucose + estradiol
UDP + ?
-
41% of the activity with testosterone as substrate
-
-
?
UDP-glucose + hydrocortisone
UDP + ?
-
11% of the activity with testosterone as substrate
-
-
?
UDP-glucose + testosterone
UDP + testosterone-17-yl beta-D-glucopyranoside
-
100% of activity
-
-
?
UDP-glucose + withanolide A
UDP + ?
-
21% of the activity with testosterone as substrate
-
-
?
UDP-glucose + withanolide U
UDP + ?
-
17% of the activity with testosterone as substrate
-
-
?
additional information
?
-
-
can conjugate glucose to C-27 position in the withanolide lactone ring and to higher positions in sterols using UDP-glucose as glucose donor, activity is enhanced following the application of salicylic acid and decreased rapidly on exposure of the plants to heat shock, suggesting a functional role of the enzyme in biotic and abiotic stresses
-
-
-
additional information
?
-
-
UDP-glucose can not be replaced by UDP-galactose
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
can conjugate glucose to C-27 position in the withanolide lactone ring and to higher positions in sterols using UDP-glucose as glucose donor, activity is enhanced following the application of salicylic acid and decreased rapidly on exposure of the plants to heat shock, suggesting a functional role of the enzyme in biotic and abiotic stresses
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.01
(5alpha, 6beta,17alpha)-5,6,17,27-tetrahydroxywithanolide
-
pH 8.0, 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LINKS TO OTHER DATABASES (specific for EC-Number 2.4.1.B4)
html completed
Information
