Information on EC 2.4.1.B3 - alpha-1,4-glucan synthase

Word Map on EC 2.4.1.B3
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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
2.4.1.B3
preliminary BRENDA-supplied EC number
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RECOMMENDED NAME
GeneOntology No.
alpha-1,4-glucan synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + [alpha-D-glucosyl-(1-3)]n = UDP + alpha-1,4-glucosyl-[alpha-D-glucosyl-(1-3)]n
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:alpha-D-(1-3)-glucan alpha-(1-4)-glucosyltransferase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
study deals with the intracellular domain of the protein
UniProt
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
265000
x * 265000, SDS-PAGE
272106
x * 272106, calculated from the deduced amino acid sequence
274335
x * 274335, calculated
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression of native and E1526A mutant enzyme in Schizosaccharomyces pombe, overexpression of native but not mutant enzyme results in accumulation of (1,4)-alpha-glucan
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
mRNAs levels of Ras homology GTPase RHO2 and enzyme gene AGS1 keep a direct relationship but the levels of Ras homology GTPase RHO1 and beta-1,3-glucan synthase FKS1 do not. Growth of cells in the Y phase in YPG medium supplemented with 5% horse serum leads to an increase in the alpha-1,3-glucan content of the cell wall, without signifi cant increase in AGS1 expression, regardless of the Y cells being grown in the presence or absence of horse serum
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
DELTA1118-1689
enzyme is not able to synthesize alpha-1,4-glucan
DELTA113-724
enzyme is able to synthesize alpha-1,4-glucan
E1526A
markedly reduced ability to complement the cell lysis phenotype and temperature sensitivity of the Schizosaccharomyces pombe ags1-1 mutant, no accumulation of alpha-1,4-glucan
E1534A
able to complement the cell lysis phenotype and temperature sensitivity of the Schizosaccharomyces pombe ags1-1 mutant
G1165A
able to complement the cell lysis phenotype and temperature sensitivity of the Schizosaccharomyces pombe ags1-1 mutant
G696S
temperature-sensitive mutation, gene is functional at 28°C but not at 36°C
K1163Q
able to complement the cell lysis phenotype and temperature sensitivity of the Schizosaccharomyces pombe ags1-1 mutant
K1422Q
reduced accumulation of alpha-1,4-glucan
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LINKS TO OTHER DATABASES (specific for EC-Number 2.4.1.B3)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)