Information on EC 2.4.1.97 - 1,3-beta-D-glucan phosphorylase

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The expected taxonomic range for this enzyme is: Ochromonas danica

EC NUMBER
COMMENTARY hide
2.4.1.97
-
RECOMMENDED NAME
GeneOntology No.
1,3-beta-D-glucan phosphorylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[(1->3)-beta-D-glucosyl]n + phosphate = [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(1->3)-beta-D-glucan:phosphate alpha-D-glucosyltransferase
Acts on a range of beta-1,3-oligoglucans, and on glucans of laminarin type. Different from EC 2.4.1.30 (1,3-beta-oligoglucan phosphorylase) and EC 2.4.1.31 (laminaribiose phosphorylase).
CAS REGISTRY NUMBER
COMMENTARY hide
37340-31-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Acacia verek
-
-
-
Manually annotated by BRENDA team
belongs to the class Chrysophyceae, golden algae
-
-
Manually annotated by BRENDA team
belongs to the class Chrysophyceae, golden algae
-
-
Manually annotated by BRENDA team
Ochromonas malhamensis
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(1,3-beta-D-glucosyl)n + phosphate
(1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
laminaribiose + glucose 1-phosphate
phosphate + laminarin
show the reaction diagram
laminarihexaose + phosphate
laminaripentaose + alpha-D-glucose 1-phosphate
show the reaction diagram
laminaripentaose + phosphate
laminaritetraose + alpha-D-glucose 1-phosphate
show the reaction diagram
laminaritetraose + phosphate
laminaritriose + alpha-D-glucose 1-phosphate
show the reaction diagram
laminaritriose + phosphate
laminaribiose + alpha-D-glucose 1-phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(1,3-beta-D-glucosyl)n + phosphate
(1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Acacia verek
-
enzyme may be a key factor in the regulation of cell-wall extension and build up by switching hydrolase and synthase activity in a balance dependent manner
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
AMP
Ochromonas malhamensis
-
low concentration: stimulation, Km: 0.04 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
AMP
Ochromonas malhamensis
-
non-competitive inhibition at high concentration
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.25
glucose
Acacia verek
-
-
12
glucose 1-phosphate
Ochromonas malhamensis
-
-
0.34 - 1.45
laminaribiose
0.47 - 1.7
Laminarin
4.1
laminaritriose
-
with phosphate, pH 7.5, 30C
4.4 - 25
phosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
400
laminaritriose
Ochromonas danica
-
with phosphate, pH 7.5, 30C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
20
AMP
Ochromonas malhamensis
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10.92
Ochromonas malhamensis
-
-
26.5
-
purified enzyme, 30C, pH 7.5
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 5.5
Ochromonas malhamensis
-
pH 5.0: 50% of activity maximum, pH 5.5: activity maximum
additional information
-
optimum at pH 5.5, the activity is low at neutral pH and almost no activity is observed at pH 8.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22.5
Ochromonas malhamensis
-
-
25 - 30
-
assay at 30C
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
16 - 27
Ochromonas malhamensis
-
about 50% of activity maximum at 16C and 27C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Acacia verek
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Acacia verek
-
bound
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
250000
-
by gel filtration, indicating a homodimer and/or a heterodimer
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
homodimer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
-
Ochromonas malhamensis
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.7 - 9.1
-
at 4C for 15 h
724604
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
stable up to 40C at pH 7.5 for 30 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing and thawing, loss of activity
Ochromonas malhamensis
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0-4C, stable for several weeks
Ochromonas malhamensis
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
-
Ochromonas malhamensis
-
purified from a cell-free extract by ammonium sulfate fractionation and 3 steps of column chromatography, 93.3 fold with a yield 16.7%, 2 mg of purified enzyme is obtained from 5 l culture
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