Information on EC 2.4.1.94 - protein N-acetylglucosaminyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.94
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RECOMMENDED NAME
GeneOntology No.
protein N-acetylglucosaminyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-D-glucosamine + [protein]-L-asparagine = UDP + [protein]-N4-(N-acetyl-D-glucosaminyl)-L-asparagine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-glucosamine:[protein]-L-asparagine beta-N-acetyl-D-glucosaminyl-transferase
The acceptor is the asparagine residue in a sequence of the form Asn-Xaa-Thr or Asn-Xaa-Ser.
CAS REGISTRY NUMBER
COMMENTARY hide
72319-34-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
mutant 2180-1A-5mnn2
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-alpha-D-glucosamine + pancreatic ribonuclease A
UDP + pancreatic ribonuclease A with N-acetylglucosamine linked to Asp
show the reaction diagram
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-
-
-
?
UDP-N-acetyl-D-glucosamine + protein
UDP + 4-N-(N-acetyl-D-glucosaminyl)-protein
show the reaction diagram
UDP-N-acetylglucosamine + pancreatic ribonuclease A
UDP + pancreatic ribonuclease A with N-acetylglucosamine linked to Asp-34
show the reaction diagram
additional information
?
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catalyses the glycosylation in vitro of the asparagine residue present in the sequence Asn, Leu, Thr
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
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catalyses the glycosylation in vitro of the asparagine residue present in the sequence Asn, Leu, Thr
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-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
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activation
Ni2+
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slight activation
additional information
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Mg2+ is ineffective
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Triton X-100
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at 0.1% and 0.2% concentrations has no effect, at a concentration of 1%, there is a 47% inhibition of enzyme activity
UDP
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at concentrations of UDP of 0.02, 0.10, and 0.2 mM, the glycosylation of ribonuclease A is inhibited to the extent of 2,15, and 27%, respectively
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CDP-choline
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activator, 2.4 mM
spermidine
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most effective activator, 10-15 mM
spermine
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activator
Triton X-100
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1.7fold at 0.04%
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE