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4-methylumbelliferyl-beta-D-GlcNAc + UDP-galactose
?
-
Hp14GalT specifically recognizes GlcNAc-terminated glycans as acceptors
-
-
?
UDP-alpha-D-galactose + 1-(2-naphthyl) 2-acetamido-2-deoxy-beta-D-glucopyranoside
UDP + ?
-
-
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-glucopyranoside
UDP + 4-methylumbelliferyl 4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside
-
-
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl-6-sulfo-N-acetyl-beta-D-glucosaminide
?
substrate with very low efficiency
-
-
?
UDP-alpha-D-galactose + 4-nitrophenyl beta-D-glucopyranoside
?
0.26% activity compared to 4-nitrophenyl N-acetyl-beta-D-glucosaminide
-
-
?
UDP-alpha-D-galactose + 4-nitrophenyl N-acetyl-beta-D-glucosaminide
UDP + 4-nitrophenyl N-acetyllactosamine
100% activity
-
-
?
UDP-alpha-D-galactose + 4-nitrophenyl xylose
UDP + 4-nitrophenyl Xylbeta(1->4)Gal
-
-
-
?
UDP-alpha-D-galactose + 6-sulfo-N-acetyl-beta-D-glucosamine
?
substrate with very low efficiency
-
-
?
UDP-alpha-D-galactose + asialo-agalacto-transferrin
?
-
-
-
-
?
UDP-alpha-D-galactose + galactosyl-beta-1,4-N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,6-(N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,3-)mannosyl-beta-1,4-N-acetylglucosaminyl-beta-1,4-(fucosyl-alpha-1,6-)N-acetylglucosaminyl-asparagine
?
-
-
-
-
?
UDP-alpha-D-galactose + GlcNAcbeta(1->2)Manalpha(1->3)GlcNAcbeta(1->2)Manalpha(1->6)Manbeta(1->4)GlcNAc2-PA
?
61% activity compared to Manalpha(1->3)GlcNAcbeta(1->2)Manalpha(1->6)Manbeta(1->4)GlcNAc2-PA
-
-
?
UDP-alpha-D-galactose + GlcNAcbeta(1->2)Manalpha(1->3)Manalpha(1->6)Manbeta(1->4)GlcNAc2-PA
?
50% activity compared to Manalpha(1->3)GlcNAcbeta(1->2)Manalpha(1->6)Manbeta(1->4)GlcNAc2-PA
-
-
?
UDP-alpha-D-galactose + GlcNAcbeta(1->3)GalNAcalpha-pNP
?
32% activity compared to 4-nitrophenyl N-acetyl-beta-D-glucosaminide
-
-
?
UDP-alpha-D-galactose + glycopeptide from porcine immunoglobulin G
?
-
-
-
-
?
UDP-alpha-D-galactose + immunoglobulin G heavy chain
?
-
-
-
-
?
UDP-alpha-D-galactose + Manalpha(1->3)GlcNAcbeta(1->2)Manalpha(1->6)Manbeta(1->4)GlcNAc2-PA
?
100% activity
-
-
?
UDP-alpha-D-galactose + N-acetyl-D-galactosamine
?
-
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
UDP-alpha-D-galactose + N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,6-(galactosyl-beta-1,4-N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,3-)mannosyl-beta-1,4-N-acetylglucosaminyl-beta-1,4-(fucosyl-alpha-1,6-)N-acetylglucosaminyl-asparagine
?
-
-
-
-
?
UDP-alpha-D-galactose + ovalbumin
UDP + ?
-
-
-
-
?
UDP-alpha-D-glucose + N-acetyl-alpha-D-glucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-galactose + (beta-1,4-galactan)n
UDP + (beta-1,4-galactan)n+1
UDP-galactose + 2-aminobenzaminated 1,4-linked beta-D-galactoheptaose
UDP + 2-aminobenzaminated 1,4-linked beta-D-galactooctaose
-
fluorogenic synthetic acceptor substrate, elongation at the reducing end
-
-
?
UDP-galactose + 2-aminobenzaminated 1,4-linked beta-D-galactohexaose
UDP + 2-aminobenzaminated 1,4-linked beta-D-galactoheptaose
-
fluorogenic synthetic acceptor substrate, elongation at the reducing end
-
-
?
UDP-galactose + 2-aminobenzaminated 1,4-linked beta-D-galactooligosaccharides
UDP + ?
-
fluorogenic synthetic acceptor substrates, enzyme transfers up to 8 galactose residues to the nonreducing ends of forming beta-1,4-linkages
-
-
?
UDP-galactose + 2-aminobenzaminated 1,4-linked beta-D-galactopentaose
UDP + 2-aminobenzaminated 1,4-linked beta-D-galactohexaose
-
fluorogenic synthetic acceptor substrate, elongation at the reducing end
-
-
?
UDP-galactose + 2-aminobenzaminated 1,4-linked beta-D-galactotetraose
UDP + 2-aminobenzaminated 1,4-linked beta-D-galactopentaose
-
fluorogenic synthetic acceptor substrate, elongation at the reducing end
-
-
?
UDP-galactose + 3-deoxy-3-fluoro-GlcNAcbeta-Bn
UDP + Galbeta(1-4)3-deoxy-3-fluoro-GlcNAcbeta-Bn
-
-
-
-
?
UDP-galactose + 4-methylumbelliferyl N-acetyl-beta-D-glucosamine
UDP + 4-methylumbelliferyl 4-O-beta-D-galactopyranosyl-N-acetyl-beta-D-glucosaminopyranoside
-
NmLgtB can use both GlcNAc- and Glc-terminated glycans as acceptor substrates
-
-
?
UDP-galactose + 6-deoxy-GlcNAcbeta-Bn
UDP + Galbeta(1-4)6-deoxy-GlcNAcbeta-Bn
-
low activity
-
-
?
UDP-galactose + 6-thio-GlcNAcbeta-Bn
UDP + Galbeta(1-4)6-thio-GlcNAcbeta-Bn
-
low activity
-
-
?
UDP-galactose + colchicoside
?
-
-
-
-
?
UDP-galactose + D-glucose
UDP + lactose
-
reaction of EC 2.4.1.22
-
-
?
UDP-galactose + ginsenoside Rg1
?
-
-
-
-
?
UDP-galactose + GlcNAcbeta(1,2)Manalpha(1,6)(GlcNAcbeta(1,2)Manalpha(1,3))Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc-PA
UDP + Galbeta1,4-GlcNAcbeta(1,2)Manalpha(1,6)(GlcNAcbeta(1,2)Manalpha(1,3))Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc-PA
-
-
-
-
?
UDP-galactose + GlcNAcbeta-Bn
UDP + Galbeta(1-4)GlcNAcbeta-Bn
-
-
-
-
?
UDP-galactose + methyl 2-acetamido-2-deoxy-beta-D-glucoside
UDP + beta-D-galactosyl-1,4-beta-1-O-methyl-2-deoxy-2-acetylamido-beta-D-glucopyranoside
-
76% of the activity with N-acetylglucosamine
-
-
?
UDP-galactose + methyl 2-bromo-acetamido-2-deoxy-beta-D-glucoside
UDP + beta-D-galactosyl-1,4-1-O-methyl-2-bromo-acetamido-2-deoxy-beta-D-glucoside
-
75% of the activity with N-acetylglucosamine
-
-
?
UDP-galactose + methyl 2-deoxy-2-benzamido-beta-D-glucoside
UDP + beta-D-galactosyl-1,4-1-O-methyl-2-deoxy-2-benzamido-beta-D-glucoside
-
16% of the activity with N-acetylglucosamine
-
-
?
UDP-galactose + N-4-toluenesulfonyl-GlcN
UDP + Galbeta(1-4)N-4-toluenesulfonyl-GlcN
-
-
-
-
?
UDP-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
UDP-galactose + N-acetylglucosamine
UDP + N-acetyllactosamine
UDP-galactose + N-butyryl-GlcNbeta-Bn
UDP + Galbeta(1-4)N-butyryl-GlcNbeta-Bn
-
-
-
-
?
UDP-galactose + N-methanesulfonyl-GlcN
UDP + Galbeta(1-4)N-methanesulfonyl-GlcN
-
-
-
-
?
UDP-galactose + N-trifluoroacetyl-GlcN
UDP + Galbeta(1-4)N-trifluoroacetyl-GlcN
-
-
-
-
?
UDP-galactose + ovalbumin
UDP + ?
-
-
-
-
?
UDP-galactose + T(GlcNAcbeta3GalNAcbeta)TTVTPTPTG
?
-
-
-
-
?
UDP-galactose + T(GlcNAcbeta6GalNAcbeta)TTVTPTPTG
?
-
-
-
-
?
UDP-galactose + T(GlcNAcbeta6[GlcNAcbeta3]GalNAcbeta)TTVTPTPTG
?
-
-
-
-
?
UDP-galactose + TT(GlcNAcbeta3GalNAcbeta)TVTPTPTG
?
-
-
-
-
?
UDP-galactose + TT(GlcNAcbeta6GalNAcbeta)TVTPTPTG
?
-
-
-
-
?
UDP-galactose + TT(GlcNAcbeta6[GlcNAcbeta3]GalNAcbeta)TVTPTPTG
?
-
-
-
-
?
UDP-galactose + TTTV (GlcNAcbeta3GalNAcbeta)TPTPTG
?
-
-
-
-
?
UDP-galactose + TTTV(GlcNAcbeta6GalNAcbeta)TPTPTG
?
-
-
-
-
?
UDP-galactose + TTTV(GlcNAcbeta6[GlcNAcbeta3]GalNAcbeta)TPTPTG
?
-
-
-
-
?
UDP-galactose + TTTVTP(GlcNAcbeta3GalNAcbeta)TPTG
?
-
-
-
-
?
UDP-galactose + TTTVTP(GlcNAcbeta6GalNAcbeta)TPTG
?
-
-
-
-
?
UDP-galactose + TTTVTPTP(GlcNAcbeta3GalNAcbeta)TG
?
-
-
-
-
?
UDP-galactose + TTTVTPTP(GlcNAcbeta6[GlcNAcbeta3]GalNAcbeta)TG
?
-
-
-
-
?
UDP-glucose + colchicoside
?
-
-
-
-
?
UDPgalactose + 2-acetamido-N-(L-aspart-4-oyl)-1,2-dideoxy-beta-glucoside
?
-
65% of the activity with N-acetylglucosamine
-
-
?
UDPgalactose + 3-acetamido-3-deoxy-D-xylose
?
-
-
-
-
?
UDPgalactose + agalacto-ovomucoid
?
-
65% of the activity with N-acetylglucosamine
-
-
?
UDPgalactose + agalacto-poly-N-acetyllactosamine
?
-
-
-
-
?
UDPgalactose + agalactokeratan
?
-
agalactokeratan from bovine cornea and nasal septum, at 5% and 13% of the activity with N-acetylglucosamine
-
-
?
UDPgalactose + alpha1-acid glycoprotein
?
-
-
-
-
?
UDPgalactose + asialo agalacto alpha1 acid glycoprotein
?
-
-
-
-
?
UDPgalactose + asialo-agalacto-alpha1-glycoprotein
?
-
42% of the activity with N-acetylglucosamine
-
-
?
UDPgalactose + asialo-agalacto-transferrin
?
-
transfer of galactose to N-acetylglucosamine residues of Asn-linked sugar chains of glycoproteins in a beta1-4linkage
-
-
?
UDPgalactose + asialogalactofetuin
?
-
-
-
-
?
UDPgalactose + chitobiose
?
-
-
-
-
?
UDPgalactose + chitotriose
?
-
-
-
-
?
UDPgalactose + degalactosylated fetuin
UDP + fetuin containing beta-1,4-galactose linkages
UDPgalactose + di-acetylchitobiose
?
-
54% of the activity with N-acetylglucosamine
-
-
?
UDPgalactose + fetuin
?
-
-
-
-
?
UDPgalactose + GlcNAcbeta-S-p-NP
UDP + Galbeta1-4 GlcNAcbeta-S-p-NP
-
-
-
?
UDPgalactose + GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha1-3Manbeta1-O(CH2)8COOCH2-mNP
UDP + Galbeta1-4 GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha1-3Manbeta1-O(CH2)8COOCH2-mNP
-
-
-
?
UDPgalactose + glucose
lactose + UDP
UDPgalactose + immunoglobulin heavy chain
?
-
-
-
-
?
UDPgalactose + lacto-N-triaosylceramide
?
-
-
-
-
?
UDPgalactose + lacto-N-triose II
?
-
-
-
-
?
UDPgalactose + N-acetamido-3-deoxy-D-glucose
?
-
-
-
-
?
UDPgalactose + N-acetyl-beta-D-glucosaminyl-glycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
UDPgalactose + N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,6-(N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,3-)mannosyl-beta-1,4-N-acetylglucosaminyl-beta-1,4-(fucosyl-alpha-1,6-)N-acetylglucosaminyl-asparagine
UDP + galactosyl-beta-1,4-N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,6-(galactosyl-beta-1,4-N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,3-)mannosyl-beta-1,4-N-acetylglucosaminyl-beta-1,4-(fucosyl-alpha-1,6-)N-acetylglucosaminyl-asparagine
-
-
galactose is transferred much faster to the N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,3-branch than to the N-acetylglucosaminyl-beta-1,2-mannosyl-alpha-1,6-branch
?
UDPgalactose + N-acetylglucosaminyl-beta-1,3-(galactosyl-beta-1,4-N-acetylglucosaminyl-beta-1,6-)galactose
UDP + galactosyl-beta-1,4-N-acetylglucosaminyl-beta-1,3-(galactosyl-beta-1,4-N-acetylglucosaminyl-beta-1,6-)galactose
-
-
-
-
?
UDPgalactose + N-acetylglucosaminyl-beta-1,3-(N-acetylglucosaminyl-beta-1,6-)galactose
UDP + galactosyl-beta-1,4-N-acetylglucosaminyl-beta-1,3-(N-acetylglucosaminyl-beta-1,6-)galactose
-
-
-
-
?
UDPgalactose + N-acetylglucosaminyl-beta-1,3-galactose
UDP + galactosyl-beta-1,4-N-acetylglucosaminyl-beta-1,3-galactose
-
-
-
-
?
UDPgalactose + N-acetylglucosaminyl-beta-1,6-galactose
UDP + galactosyl-beta-1,4-N-acetylglucosaminyl-beta-1,6-galactose
-
-
-
-
?
UDPgalactose + ovalbumin
?
UDPgalactose + ovomucoid
UDP + ovomucoid with beta-1,4-bound galactose
-
-
-
?
UDPgalactose + p-nitrophenyl 2-acetamido-2-deoxy-beta-glucoside
?
UDPgalactose + tri-N-acetylchitotriose
?
-
64% of the activity with N-acetylglucosamine
-
-
?
UDPgalactose + UDPglucose
UDP + lactose
-
-
-
?
additional information
?
-
UDP-alpha-D-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-galactose + (beta-1,4-galactan)n
UDP + (beta-1,4-galactan)n+1
-
enzyme elongates beta-1,4-galactan side chains of rhamnogalacturonan I components of the cell wall pectin
-
-
?
UDP-galactose + (beta-1,4-galactan)n
UDP + (beta-1,4-galactan)n+1
-
acceptor substrate is modified rhamnogalacturonan I
-
-
?
UDP-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
enzyme is highly specific for UDP-galactose as donor substrate, 100fold less active with UDP-glucose
-
-
?
UDP-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
specific for UDP-galactose
-
-
?
UDP-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
in presence of alpha-lactalbumin, the enzyme catalyzes reaction of EC 2.4.1.22
-
-
?
UDP-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
synthesis of Galbeta1,4GlcNAc groups in N-linked sugar chains of glycoproteins involved in many physiological functions, e.g. sperm-egg interactions, cell migration, and embryonic development, enzyme expression is highly increased during cycloheximide-induced cell apoptosis in liver carcinoma
-
-
?
UDP-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
enzyme is highly specific for UDP-galactose as donor substrate
-
-
?
UDP-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
substrate binding structure
-
-
?
UDP-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-galactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-galactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
partial reaction of EC 2.4.1.22
-
-
?
UDP-galactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
?
UDP-galactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-galactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
wild-type enzyme, residue I285 alone is responsible for determination of enzyme substrate specificity, overview, active site structure, overview
-
-
?
UDP-galactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-galactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
?
UDP-galactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
?
UDP-galactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-GalNAc + GlcNAc
?
-
at high concentrations of alpha-lactalbumin
-
-
?
UDP-GalNAc + GlcNAc
?
-
transfer of GalNAc is only 1% of galactose transfer in wild type enzyme. Mutant enzyme Y289L exhibits nearly 100% of the galactose transferase activity
-
-
?
UDPgalactose + degalactosylated fetuin
UDP + fetuin containing beta-1,4-galactose linkages
-
-
-
?
UDPgalactose + degalactosylated fetuin
UDP + fetuin containing beta-1,4-galactose linkages
-
-
-
-
?
UDPgalactose + degalactosylated fetuin
UDP + fetuin containing beta-1,4-galactose linkages
-
-
-
-
?
UDPgalactose + degalactosylated fetuin
UDP + fetuin containing beta-1,4-galactose linkages
-
-
-
-
?
UDPgalactose + glucose
lactose + UDP
-
in presence of alpha-lactalbumin
-
?
UDPgalactose + glucose
lactose + UDP
-
in presence of alpha-lactalbumin
-
?
UDPgalactose + glucose
lactose + UDP
-
in presence of alpha-lactalbumin
-
-
?
UDPgalactose + glucose
lactose + UDP
-
in presence of alpha-lactalbumin
-
?
UDPgalactose + glucose
lactose + UDP
-
in presence of alpha-lactalbumin
-
?
UDPgalactose + glucose
lactose + UDP
-
in presence of alpha-lactalbumin
-
-
?
UDPgalactose + glucose
lactose + UDP
enzyme has no lactose synthase activity in presence of alpha-lactalbumin
-
-
?
UDPgalactose + glucose
lactose + UDP
-
in presence of alpha-lactalbumin
-
?
UDPgalactose + glucose
lactose + UDP
-
in presence of alpha-lactalbumin
-
?
UDPgalactose + glucose
lactose + UDP
-
in presence of alpha-lactalbumin
-
?
UDPgalactose + glucose
lactose + UDP
-
in presence of alpha-lactalbumin
-
?
UDPgalactose + glucose
lactose + UDP
-
in presence of alpha-lactalbumin
-
?
UDPgalactose + glucose
lactose + UDP
-
in presence of alpha-lactalbumin
-
?
UDPgalactose + N-acetyl-beta-D-glucosaminyl-glycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
glycoproteins containing terminal nonreducing N-acetylglucosaminyl units
-
-
?
UDPgalactose + N-acetyl-beta-D-glucosaminyl-glycopeptide
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
glycopeptide prepared from porcine IgG immunoglobulin
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
7% of the activity with GlcNAcbeta-S-pNP
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDPgalactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
-
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
the enzyme facilitates sperm binding to the oocyte zona pellucida
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
enzyme participates in the biosynthesis of the oligosaccharide structures of glycoproteins and glycolipids
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
enzyme functions in the coordinate biosynthesis of complex oligosaccharides, proposed to function in intercellular recognition and/or adhesion
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
biosynthesis of keratan sulfate-like polysaccharides
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
the enzyme may be involved in the synthesis of poly-N-acetyllactosamine, lacto-N-neotetraose and probably lacto-N-neotetraosylceramide in addition to the formation of the Galbeta1-4GlcNAc group of glycoprotein sugar chains and lactose
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
main enzyme responsible for the transfer of galactose residues from UDPgalactose into terminal N-acetylglucosamine residues of complex-type oligosaccharides in newly synthesized glycoproteins in the Golgi apparatus. Deficiency of UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase I causes the congenital disorder of glycosylation type IId, a severe neurologic disease characterized by a hydrocephalus, myopathy and blood-clotting defects
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
the enzyme is involved in the biosynthesis of a variety of carbohydrate structures in glycoproteins and glycolipids
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
biosynthesis of carbohydrate moieties of glycoproteins and glycolipids, role in intercellular recognition and adhesion
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
-
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
the soluble enzyme form from the luminal fluid of the epididymis is suggested to play a role on sperm maturation
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
the enzyme may be involved in the synthesis of plasma glycoproteins by the liver during secretion, and may possibly be required for secretion of these proteins
-
-
?
UDPgalactose + ovalbumin
?
-
-
-
-
?
UDPgalactose + ovalbumin
?
-
-
-
-
?
UDPgalactose + ovalbumin
?
-
-
-
-
?
UDPgalactose + p-nitrophenyl 2-acetamido-2-deoxy-beta-glucoside
?
-
67% of the activity with N-acetylglucosamine
-
-
?
UDPgalactose + p-nitrophenyl 2-acetamido-2-deoxy-beta-glucoside
?
-
-
-
-
?
additional information
?
-
the enzyme does not exhibit beta-1,4-galactosyltransferase activity. No activity with 4-nitrophenyl N-acetyl-beta-D-galactosaminide, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-D-mannopyranoside, GalNAcbeta(1->4)GlcNAcbeta-pNP, and Galbeta(1->4)GlcNAcbeta-pNP
-
-
-
additional information
?
-
-
regioselectivity towards specific C(4)glucose OH group in the complex protopanaxadiol glycoside ginsenoside Rb1
-
-
?
additional information
?
-
-
enzyme also catalyzes unusual galactosyl transfer to the 3-OH position of L-sugars
-
-
?
additional information
?
-
-
enzyme also catalyzes transfer of glucose from UDPglucose to N-acetylglucosamine
-
-
?
additional information
?
-
-
enzyme interacts with alpha-lactalbumin to form the lactose synthase enzyme complex, structural basis for catalytic mechanism, overview
-
-
?
additional information
?
-
-
substrate specificity in presence or absence of alpha-lactalbumin, overview, the 'specifier' protein alpha-lactalbumin, which interacts with beta-1,4-GalT forming the lactose synthase complex, EC 2.4.1.22, is not necessary when the acceptors are different glucopyranosides, EC 2.4.1.38, and, in some cases, it can even have an inhibitory effect, e.g. in reaction with the complex glucosides ginsenoside Rg1 and colchicoside, overview
-
-
?
additional information
?
-
-
the enzyme catalyzes the transfer of Gal from UDP-Gal to GlcNAc-terminating acceptors with inversion of configuration of the glycosidic linkage, in presence of beta-lactalbumin, the enzyme changes its specificity to become lactose synthase, EC 2.4.1.22, and transfers Gal to glucose to synthesize lactose, substrate specificity, e.g. with GlcNAc-terminating O-glycopeptides, no activity with N-trimethylacetyl-GlcN, N,N-dimethyl-GlcN, N-isopropyl-GlcN, and N-(4-MeOBn)GlcN, overview
-
-
?
additional information
?
-
-
substrate specificity, coupling of 6-azido-6-deoxy D-galactose to a GlcNAc-terminated substrate, method development for usage of the enzyme reaction for tagging glycoproteins carrying terminal GlcNAc, overview
-
-
?
additional information
?
-
the enzyme is part of the endoplasmic reticulum and Golgi glycosylation network, glycosyltransferase metabolism, overview
-
-
?
additional information
?
-
-
the preexisting alpha-lactalbumin-binding site iss utilized during mammalian evolution to synthesize lactose in the mammary gland during lactation
-
-
?
additional information
?
-
-
Hp1-4GalT can not use Glc, alpha-linked GlcNAc, and N-modified GlcNAc as acceptors but it can use 6-O-sulfated GlcNAc such as GlcNAc6S and GlcNAc6SbetaProN3 as suitable acceptor substrates
-
-
?
additional information
?
-
-
the enzyme utilizes different glycoproteins and glycolipids as substrates, patients with rheumatoid arthritis have a higher content of an acidic isoform compared to healthy individuals, the isoform is not associated with inflammation per se but specifically with rheumatoid arthritis
-
-
?
additional information
?
-
-
analysis of cell surface sugar chains in the cancer cell lines, overview
-
-
?
additional information
?
-
-
UDP-glucose and UDP-N-acetylgalactosamine are poor substrates, the latter due to Tyr286 which is a sterical hindrance and traps hydrolyzed GalNAc in the oxocarbenium-ion-like state, enzyme interacts with alpha-lactalbumin to form the lactose synthase enzyme complex
-
-
?
additional information
?
-
-
tumor beta-1,4-galactosyltransferase IV overexpression is closely associated with colorectal cancer metastasis and poor prognosis, relationships between tumor beta-1,4-GT-IV overexpression and clinicopathologic characteristics, overview
-
-
?
additional information
?
-
-
beta-1,4-GTs are a family of glycosyltransferases responsible for biosynthesizing N-acetyllactosamine by the transfer of a galactose from UDP-galactose to the terminal N-acetylgluosamine of acceptor sugars in glycoproteins or glycolipids with a beta-1,4-linkage
-
-
?
additional information
?
-
-
substrate specificity of wild-type and mutant Y289L enzymes, overview
-
-
?
additional information
?
-
beta1,4-galactosyltransferase II is one of the enzymes transferring galactose to the terminal N-acetylglucosamine of complex-type N-glycans. Beta1,4GalT II might serve as a target gene of p53 transcription factor during adriamycin-induced HeLa cell apoptosis, mechanisms of its expression regulation, overview
-
-
?
additional information
?
-
-
no activity with 5-(indol-4-yl)-UDP-alpha-D-galactose
-
-
-
additional information
?
-
-
NmLgtB can use Glc, both alpha- and beta-linked GlcNAc, and N-modified but not 6-O-sulfated GlcNAc as acceptors
-
-
?
additional information
?
-
-
the enzyme is involved in regulation of carbohydrate composition of milk during lactation, endocrine enzyme regulation, overview
-
-
?
additional information
?
-
-
the enzyme is one of the key molecules on the sperm surface, and is likely to be involved in binding to the egg coat, the zona pelludica, to mediate sperm-egg interaction
-
-
?
additional information
?
-
-
no activity with penta- and heptasaccharides generated from rhamnogalacturonan I, product determination by mass spectrometry, NMR, and enzymatic digestion analysis
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
UDP-galactose + (beta-1,4-galactan)n
UDP + (beta-1,4-galactan)n+1
-
enzyme elongates beta-1,4-galactan side chains of rhamnogalacturonan I components of the cell wall pectin
-
-
?
UDP-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
UDP-galactose + N-acetylglucosamine
UDP + N-acetyllactosamine
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
additional information
?
-
UDP-alpha-D-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
synthesis of Galbeta1,4GlcNAc groups in N-linked sugar chains of glycoproteins involved in many physiological functions, e.g. sperm-egg interactions, cell migration, and embryonic development, enzyme expression is highly increased during cycloheximide-induced cell apoptosis in liver carcinoma
-
-
?
UDP-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-galactose + N-acetyl-D-glucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-galactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-galactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
?
UDP-galactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-galactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDP-galactose + N-acetylglucosamine
UDP + N-acetyllactosamine
-
-
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
-
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
the enzyme facilitates sperm binding to the oocyte zona pellucida
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
enzyme participates in the biosynthesis of the oligosaccharide structures of glycoproteins and glycolipids
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
enzyme functions in the coordinate biosynthesis of complex oligosaccharides, proposed to function in intercellular recognition and/or adhesion
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
biosynthesis of keratan sulfate-like polysaccharides
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
the enzyme may be involved in the synthesis of poly-N-acetyllactosamine, lacto-N-neotetraose and probably lacto-N-neotetraosylceramide in addition to the formation of the Galbeta1-4GlcNAc group of glycoprotein sugar chains and lactose
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
main enzyme responsible for the transfer of galactose residues from UDPgalactose into terminal N-acetylglucosamine residues of complex-type oligosaccharides in newly synthesized glycoproteins in the Golgi apparatus. Deficiency of UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase I causes the congenital disorder of glycosylation type IId, a severe neurologic disease characterized by a hydrocephalus, myopathy and blood-clotting defects
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
the enzyme is involved in the biosynthesis of a variety of carbohydrate structures in glycoproteins and glycolipids
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
biosynthesis of carbohydrate moieties of glycoproteins and glycolipids, role in intercellular recognition and adhesion
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
-
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
the soluble enzyme form from the luminal fluid of the epididymis is suggested to play a role on sperm maturation
-
-
?
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides
?
-
the enzyme may be involved in the synthesis of plasma glycoproteins by the liver during secretion, and may possibly be required for secretion of these proteins
-
-
?
additional information
?
-
the enzyme is part of the endoplasmic reticulum and Golgi glycosylation network, glycosyltransferase metabolism, overview
-
-
?
additional information
?
-
-
the preexisting alpha-lactalbumin-binding site iss utilized during mammalian evolution to synthesize lactose in the mammary gland during lactation
-
-
?
additional information
?
-
-
the enzyme utilizes different glycoproteins and glycolipids as substrates, patients with rheumatoid arthritis have a higher content of an acidic isoform compared to healthy individuals, the isoform is not associated with inflammation per se but specifically with rheumatoid arthritis
-
-
?
additional information
?
-
-
tumor beta-1,4-galactosyltransferase IV overexpression is closely associated with colorectal cancer metastasis and poor prognosis, relationships between tumor beta-1,4-GT-IV overexpression and clinicopathologic characteristics, overview
-
-
?
additional information
?
-
beta1,4-galactosyltransferase II is one of the enzymes transferring galactose to the terminal N-acetylglucosamine of complex-type N-glycans. Beta1,4GalT II might serve as a target gene of p53 transcription factor during adriamycin-induced HeLa cell apoptosis, mechanisms of its expression regulation, overview
-
-
?
additional information
?
-
-
the enzyme is involved in regulation of carbohydrate composition of milk during lactation, endocrine enzyme regulation, overview
-
-
?
additional information
?
-
-
the enzyme is one of the key molecules on the sperm surface, and is likely to be involved in binding to the egg coat, the zona pelludica, to mediate sperm-egg interaction
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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1,6-dithio-N-butyryl-GlcNbeta-(2-naphthyl)
-
45% inhibition at 1.0 mM
1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-5-(1-methyl-1H-indol-4-yl)pyrimidine-2,4(1H,3H)-dione
-
-
1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-5-(1H-indazol-4-yl)pyrimidine-2,4(1H,3H)-dione
-
-
1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-5-(1H-indol-4-yl)pyrimidine-2,4(1H,3H)-dione
-
-
1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-5-(1H-indol-5-yl)pyrimidine-2,4(1H,3H)-dione
-
-
1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-5-(1H-indol-6-yl)pyrimidine-2,4(1H,3H)-dione
-
-
1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-5-(2-(4-methylpiperazin-1-yl)pyridin-4-yl)pyrimidine-2,4(1H,3H)-dione
-
-
1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-5-(isoquinolin-4-yl)pyrimidine-2,4(1H,3H)-dione
-
-
1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-5-(pyridin-3-yl)pyrimidine-2,4(1H,3H)-dione
-
-
1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-5-(pyridin-4-yl)pyrimidine-2,4(1H,3H)-dione
-
-
1-thio-N-butyryl-GlcNbeta-(2-naphthyl)
-
uncompetitive, complete inhibition at 1.0 mM, 85% inhibition at 0.2 mM
1-thioGlcNAcbeta-(2-naphthyl)
-
91% inhibition at 1.0 mM
2-((5-(1,2,3,4-tetrahydro-1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-2,4-dioxopyrimidin-5-yl)thiophen-2-yl)methylamino)-3-(1H-indol-3-yl)propanoic acid
-
-
2-((5-(1,2,3,4-tetrahydro-1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-2,4-dioxopyrimidin-5-yl)thiophen-2-yl)methylamino)-4-methylpentanoic acid
-
-
2-((5-(1,2,3,4-tetrahydro-1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-2,4-dioxopyrimidin-5-yl)thiophen-2-yl)methylamino)-6-aminohexanoic acid
-
-
2-((5-(1,2,3,4-tetrahydro-1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-2,4-dioxopyrimidin-5-yl)thiophen-2-yl)methylamino)acetic acid
-
-
5-(1,2,3,4-tetrahydro-1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-2,4-dioxopyrimidin-5-yl)thiophene-2-carbaldehyde
-
-
6-thio-N-butyryl-GlcNbeta-(2-naphthyl)
-
19% inhibition at 1.0 mM
acetone
-
20% v/v, 37% inhibition
acetonitrile
-
20% v/v, complete inhibition
alpha1-Acid glycoprotein
-
above 1.4 mM with respect to acceptor sites
-
ammonium
enzyme activity is decreased in ammonium treated cell culture
Ca2+
less than 4% activity in the presence of Ca2+ions
Co2+
less than 4% activity in the presence of Co2+ions
D-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol
-
potent inhibitor
Dimethylsulfoxide
-
20% v/v, 62% inhibition
ethanol
-
20% v/v, 42% inhibition
GlcNAcbeta-(2-naphthyl)
-
92% inhibition at 1.0 mM
Mg2+
less than 4% activity in the presence of Mg2+ions
N,N-Dimethylformamide
-
20% v/v, 84% inhibition
N-Acetylimidazole
-
activity is partially restored by treatment with hydroxylamine
N-butyryl-GlcNbeta-(2-naphthyl)
-
87% inhibition at 1.0 mM
N-methylpyrrolidone
-
20% v/v, 9% inhibition
p-hydroxymercuribenzoate
-
-
p-nitrophenyl 2-acetamido-2-deoxy-beta-glucoside
-
competitively inhibits the transfer of galactose to glycoprotein substrates
phosphatidylethanolamine
-
-
tetrahydrofuran
-
20% v/v, complete inhibition
UMP
-
competitively inhibits the transfer of galactose to glycoprotein substrates
alpha-lactalbumin
-
inhibits reaction with UDPgalactose and N-acetylglucosamine
-
alpha-lactalbumin
-
inhibits the GalNAc transferase activity of the enzyme
-
alpha-lactalbumin
-
inhibits reaction with UDPgalactose and N-acetylglucosamine
-
alpha-lactalbumin
-
partially inhibits reaction with UDPgalactose and asialo-agalacto-transferrin
-
alpha-lactalbumin
-
inhibits N-acetyllactosamine synthesis in plasma membrane fraction
-
alpha-lactalbumin
-
inhibits reaction with UDPgalactose and N-acetylglucosamine
-
Cu2+
-
complete inhibition in presence of Mn2+
Cu2+
-
complete inhibition in presence of Mn2+
EDTA
-
-
N-acetylglucosamine
-
above 10 mM
N-acetylglucosamine
-
competitively inhibits the transfer of galactose to glycoprotein substrates
phosphatidic acid
-
-
UDP
-
treatment with periodate-cleaved UDP and NaCNBH3 results in a loss of 80% of enzyme activity, which is largely prevented by UDP-galactose
UDP
-
competitively inhibits the transfer of galactose to glycoprotein substrates
Zn2+
-
complete inhibition in presence of Mn2+
Zn2+
-
complete inhibition in presence of Mn2+
additional information
-
the enzyme is totally inactivated by iodination with lactoperoxidase, EC 1.11.1.7. Substrates protect against inactivation
-
additional information
-
inhibitor synthesis and potency, overview
-
additional information
-
not inhibited by 1-(2-naphthyl) 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
additional information
in vivo galactosylation and sialylation inhibition is mainly due to decreased gene expression of galactosyltransferase, sialyltransferase, and CMP-sialic acid transporter and not due to sialidase
-
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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0.163
1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-5-(1-methyl-1H-indol-4-yl)pyrimidine-2,4(1H,3H)-dione
Homo sapiens
-
pH and temperature not specified in the publication
1
1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-5-(1H-indazol-4-yl)pyrimidine-2,4(1H,3H)-dione
Homo sapiens
-
IC50 above 1.0 mM, pH and temperature not specified in the publication
0.207
1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-5-(1H-indol-4-yl)pyrimidine-2,4(1H,3H)-dione
Homo sapiens
-
pH and temperature not specified in the publication
1
1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-5-(1H-indol-5-yl)pyrimidine-2,4(1H,3H)-dione
Homo sapiens
-
IC50 above 1.0 mM, pH and temperature not specified in the publication
0.25
1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-5-(1H-indol-6-yl)pyrimidine-2,4(1H,3H)-dione
Homo sapiens
-
pH and temperature not specified in the publication
1
1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-5-(2-(4-methylpiperazin-1-yl)pyridin-4-yl)pyrimidine-2,4(1H,3H)-dione
Homo sapiens
-
IC50 above 1.0 mM, pH and temperature not specified in the publication
1
1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-5-(isoquinolin-4-yl)pyrimidine-2,4(1H,3H)-dione
Homo sapiens
-
IC50 above 1.0 mM, pH and temperature not specified in the publication
1
1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-5-(pyridin-3-yl)pyrimidine-2,4(1H,3H)-dione
Homo sapiens
-
IC50 above 1.0 mM, pH and temperature not specified in the publication
1
1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-5-(pyridin-4-yl)pyrimidine-2,4(1H,3H)-dione
Homo sapiens
-
IC50 above 1.0 mM, pH and temperature not specified in the publication
0.284 - 1
2-((5-(1,2,3,4-tetrahydro-1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-2,4-dioxopyrimidin-5-yl)thiophen-2-yl)methylamino)-3-(1H-indol-3-yl)propanoic acid
1
2-((5-(1,2,3,4-tetrahydro-1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-2,4-dioxopyrimidin-5-yl)thiophen-2-yl)methylamino)-4-methylpentanoic acid
Homo sapiens
-
IC50 above 1.0 mM, pH and temperature not specified in the publication
1
2-((5-(1,2,3,4-tetrahydro-1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-2,4-dioxopyrimidin-5-yl)thiophen-2-yl)methylamino)-6-aminohexanoic acid
Homo sapiens
-
IC50 above 1.0 mM, pH and temperature not specified in the publication
1
2-((5-(1,2,3,4-tetrahydro-1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-2,4-dioxopyrimidin-5-yl)thiophen-2-yl)methylamino)acetic acid
Homo sapiens
-
IC50 above 1.0 mM, pH and temperature not specified in the publication
1
5-(1,2,3,4-tetrahydro-1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-2,4-dioxopyrimidin-5-yl)thiophene-2-carbaldehyde
Homo sapiens
-
IC50 above 1.0 mM, pH and temperature not specified in the publication
0.284
2-((5-(1,2,3,4-tetrahydro-1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-2,4-dioxopyrimidin-5-yl)thiophen-2-yl)methylamino)-3-(1H-indol-3-yl)propanoic acid
Homo sapiens
-
pH and temperature not specified in the publication
1
2-((5-(1,2,3,4-tetrahydro-1-((2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl)-2,4-dioxopyrimidin-5-yl)thiophen-2-yl)methylamino)-3-(1H-indol-3-yl)propanoic acid
Homo sapiens
-
IC50 above 1.0 mM, pH and temperature not specified in the publication
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C134S
-
complete loss of activity
C342S
-
33fold increase in the apparent Km-value for UDPgalactose
D254E
-
0.01% of the activity of the wild-type enzyme
D254N
-
0.01% of the activity of the wild-type enzyme
D320A
-
when partially activated by Mn2+ binding to the primary site, can be further activated by Co2+ or inhibited by Ca2+, an effect that is the opposite of what is observed with the wild-type enzyme
D320E
-
when partially activated by Mn2+ binding to the primary site, can be further activated by Co2+ or inhibited by Ca2+, an effect that is the opposite of what is observed with the wild-type enzyme
D320N
-
when partially activated by Mn2+ binding to the primary site, can be further activated by Co2+ or inhibited by Ca2+, an effect that is the opposite of what is observed with the wild-type enzyme
E317A
-
when partially activated by Mn2+ binding to the primary site, can be further activated by Co2+ or inhibited by Ca2+, an effect that is the opposite of what is observed with the wild-type enzyme
E317D
-
when partially activated by Mn2+ binding to the primary site, can be further activated by Co2+ or inhibited by Ca2+, an effect that is the opposite of what is observed with the wild-type enzyme
E317Q
-
when partially activated by Mn2+ binding to the primary site, can be further activated by Co2+ or inhibited by Ca2+, an effect that is the opposite of what is observed with the wild-type enzyme
H347D
-
in presence of Mn2+ retains 0.02% of wild-type enzyme activity, in presence of Co2+ retains 0.085% of wild-type enzyme activity
H347E
-
in presence of Mn2+ retains 0.1% of wild-type enzyme activity, in presence of Co2+ retains 0.4% of wild-type enzyme activity
H347N
-
in presence of Mn2+ retains 0.07% of wild-type enzyme activity, in presence of Co2+ retains 0.36% of wild-type enzyme activity
H347Q
-
in presence of Mn2+ retains 0.28% of wild-type enzyme activity, in presence of Co2+ retains 1.21% of wild-type enzyme activity
M344E
-
site-directed mutagenesis, altered metal ion specificity compared to the wild-type enzyme
M344S
-
site-directed mutagenesis, altered metal ion specificity compared to the wild-type enzyme
Y289N
-
mutation enhances GalNAc-transferase activity. Km for GlcNAc is increased compared to the wild type
DELTA1-70
-
crystallization of a refolded Drosophila Cd7 protein coding the catalytic domain (Cd7) sequence (residues 71-322) is not successful
DELTA1-70/DELTA312-322
-
a DNA fragment bearing the Cd7 catalytic domain and C-terminal 11-amino acid deletion (Cd7DELTAC) sequence (residues 71-311) shows no difference in catalytic activity or folding but crystalization is not possible
I285Y
-
site-directed mutagenesis, the mutation converts the betaGALNAcT1 enzyme into an efficient beta4Gal-T1, the N-acetylgalactosaminyltransferase activity is reduced by nearly 1000fold, while the galactosyltransferase activity is enhanced by 80fold
D315A
-
the mutant shows about 90% of wild type activity
H343A
-
the mutant shows about 3% of wild type activity
M340E
-
the mutant shows about 85% of wild type activity
M340H
-
the mutant shows about 10% of wild type activity
Y289L/C342T
-
site-directed mutagenesis, the mutant is able to transfer GalNAc from the sugar donor UDP-GalNAc to the acceptor, GlcNAc, with efficiency as good as that of galactose from UDP-Gal, in contrast to the wild-type enzyme, mutant substrate specificity with different donor substrate and oligosaccharides as acceptor substrates, mass spectrometry product analysis, overview, the C342T mutation does not alter enzyme activity, but increases the enzyme stability at room temperature
W314A
-
site-directed mutagenesis, mutant shows highly reduced activity, i.e. 0.6% of wild-type galactosyltransferase activity, substrate binding, and reduced binding to the effector alpha-lactalbumin as well as reduced susceptibility to cleavage by proteases Glu-C and Lys-C compared to the wild-type enzyme, overview
M344A
-
in presence of Mn2+ retains 54.5% of wild-type enzyme activity, in presence of Co2+ retains 6.15% of wild-type enzyme activity
M344A
-
site-directed mutagenesis, altered metal ion specificity compared to the wild-type enzyme
M344H
-
mutant prefers Mg2+ instead of Mn2+ which is preferred by the wild-type enzyme, with Mn2+ the mutant is arrested in the closed inactive conformation
M344H
-
site-directed mutagenesis, the mutant shows altered conformational changes upon binding of Mn2+ and substrates or substrate analogues compared to the wild-type enzyme, it forms the closed conformation with bound Mn2+ and UDP-hexanolamine, loss of 98% of Mn2+ binding activity, increased activity with Mg2+
M344Q
-
in presence of Mn2+ retains 15.37% of wild-type enzyme activity, in presence of Co2+ retains 31.08% of wild-type enzyme activity
M344Q
-
site-directed mutagenesis, altered metal ion specificity compared to the wild-type enzyme
Y289I
-
mutation enhances GalNAc-transferase activity. Km for GlcNAc is increased compared to the wild type
Y289I
-
mutant shows high activity with UDP-GalNAc in contrary to the wild-type enzyme
Y289L
-
mutation enhances GalNAc-transferase activity. Km for GlcNAc is incereased compared to the wild type
Y289L
-
mutant shows high activity with UDP-GalNAc in contrary to the wild-type enzyme
additional information
-
N-terminal truncated forms of the enzyme between residues 1-129, do not show any significant difference in the apparent Km-values towards N-acetylglucosamine or linear oligosaccharide acceptors, e.g. for chitobiose and chitotriose, or for the nucleotide donor UDPgalactose. The binding behaviour of N-terminal and C-terminal fragments of the enzyme towards the N-acetylglucosamine-agarose and UDP-agarose columns differ, the former binds preferentially to the N-acetylglucosamine-columns, while the latter binds to UDP-agarose columns via Mn2+
additional information
-
mutations of Asp318 and Asp319 abolish enzyme activity
additional information
-
a construct bearing the N-terminal peptides from bovine Cd1 (residues 143175 (P1)) fused with peptide with the Cd7DELTAC protein (last 11 amino acids deleted) shows no impact on the catalytic activity nor the folding efficiency. This construct is used for successful crystallization
additional information
-
the enzyme transfected 7721 hepatocarcinoma cell line is more susceptible to cycloheximide-induced apoptosis than the wild-type cells, cells show increased enzyme activity and expression of Galbeta1,4GlcNAc groups on the cell surface
additional information
decreasing the expression of beta1,4GalT II using RNA interference inhibits p53-mediated HeLa cell apoptosis induced by adriamycin
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