Information on EC 2.4.1.86 - N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferaselceramide beta-galactosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.86
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RECOMMENDED NAME
GeneOntology No.
N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferaselceramide beta-galactosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminyl-R = UDP + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
lacto-series glycosphingolipids biosynthesis
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biosynthesis of Lewis epitopes (H. pylori)
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ABH and Lewis epitopes biosynthesis from type 1 precursor disaccharide
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Glycosphingolipid biosynthesis - lacto and neolacto series
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-galactose:N-acetyl-beta-D-glucosaminyl-R 3-beta-D-galactosyltransferase
The enzyme transfers galactose from UDP-alpha-D-galactose to the 3-position of substrates with a non-reducing terminal N-acetyl-beta-D-glucosamine (beta-GlcNAc) residue. It can act on both glycolipids and glycoproteins, generating a structure known as the type 1 histo-blood group antigen precursor.
CAS REGISTRY NUMBER
COMMENTARY hide
9073-46-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
embryo
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDPgalactose + GalNAcbeta1-4Galbeta1-4Glcbeta1-1ceramide
UDP + Galbeta1,4-GalNAcbeta1-4Galbeta1-4Glcbeta1-1ceramide
show the reaction diagram
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45% of the activity with N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-D-glucosylceramide
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?
UDPgalactose + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-D-glucosylceramide
UDP + 1,4-beta-D-galactosyl-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-D-glucosylceramide
show the reaction diagram
additional information
?
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UDPglucose cannot substitute for UDPgalactose, Tay-Sachs ganglioside (GM2) is a poor galactose acceptor
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDPgalactose + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-D-glucosylceramide
UDP + 1,4-beta-D-galactosyl-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-D-glucosylceramide
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
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6-7% of the activity with Mn2+
Co2+
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6-7% of the activity with Mn2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
diisothiocyanato stilbene 4,4-disulfonate
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irreversible inhibition by binding to a UDP binding site
EDTA
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complete inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
deoxycholate
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required, most effective detergent
Detergent
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required for in vitro activity, deoxycholate is most effective
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.034 - 0.5
N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-D-glucosylceramide
0.12 - 0.25
UDPgalactose
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.344
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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specific activity is reduced by 50% soon after birth and then remains practically the same with development
Manually annotated by BRENDA team
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during progression of apoptosis with increasing concentrations of L-1-phenyl-2-hexadecanoylamino-3-morpholino-1-propanol, the enzyme activity is decreased significantly. Theses changes in specific activity could be the resultant of decreased gene expression of the enzyme
Manually annotated by BRENDA team
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mineral oil induced
Manually annotated by BRENDA team
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SV40-transformed glial cell culture derived from the cerebrum of a human fetus with Tay-Sachs disease specific cell type
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
the purified enzyme is partially stabilized in presence of phospholipid liposomes
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression as a soluble glutathione-S-transferase fusion protein in Escherichia coli
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