Information on EC 2.4.1.71 - arylamine glucosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.71
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RECOMMENDED NAME
GeneOntology No.
arylamine glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + an arylamine = UDP + an N-D-glucosylarylamine
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
metabolism of disaccharids
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:arylamine N-D-glucosyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
37277-72-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
TDP-glucose + an arylamine
TDP + an N-D-glucosylarylamine
show the reaction diagram
-
-
-
?
UDP-glucose + 2,3-dichloroaniline
UDP + N-(2,3-dichlorophenyl)-glucosylamine
show the reaction diagram
-
-
-
-
?
UDP-glucose + 2,4-dibromoaniline
UDP + N-(2,4-dibromophenyl)-glucosylamine
show the reaction diagram
-
-
-
-
?
UDP-glucose + 2,4-dichloroaniline
UDP + N-(2,4-dichlorophenyl)-glucosylamine
show the reaction diagram
-
-
-
-
?
UDP-glucose + 2,5-dibromoaniline
UDP + N-(2,5-dibromophenyl)-glucosylamine
show the reaction diagram
-
-
-
-
?
UDP-glucose + 2,5-dichloroaniline
UDP + N-(2,5-dichlorophenyl)-glucosylamine
show the reaction diagram
-
-
-
-
?
UDP-glucose + 3,4-dichloroaniline
UDP + N-(3,4-dichlorophenyl)-glucosylamine
show the reaction diagram
UDP-glucose + 3,5-dichloroaniline
UDP + N-(3,5-dichlorophenyl)-glucosylamine
show the reaction diagram
-
-
-
-
?
UDP-glucose + 3-amino-2,5-dichlorobenzoic acid
UDP + 3-glucosylamino-2,5-dichlorobenzoic acid
show the reaction diagram
-
-
-
-
?
UDP-glucose + 3-aminobenzoic acid
UDP + 3-glucosylaminobenzoic acid
show the reaction diagram
-
-
-
-
?
UDP-glucose + 3-chloroaniline
UDP + N-(3-chlorophenyl)-glucosylamine
show the reaction diagram
-
31% efficiency compared to 3,4-dichloroaniline
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-
?
UDP-glucose + 3-nitroaniline
UDP + N-(3-nitrophenyl)-glucosylamine
show the reaction diagram
-
-
-
-
?
UDP-glucose + 4-bromoaniline
UDP + N-(4-bromophenyl)-glucosylamine
show the reaction diagram
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28% efficiency compared to 3,4-dichloroaniline
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-
?
UDP-glucose + 4-chloroaniline
UDP + N-(4-chlorophenyl)-glucosylamine
show the reaction diagram
-
71% efficiency compared to 3,4-dichloroaniline
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-
?
UDP-glucose + 5-amino-2,3-dichlorobenzoic acid
UDP + 5-glucosylamino-2,3-dichlorobenzoic acid
show the reaction diagram
-
-
-
-
?
UDP-glucose + an arylamine
UDP + an N-D-glucosylarylamine
show the reaction diagram
-
involved in N-glucosylarylamines biosynthesis, may be participate in arylamine herbicide metabolism
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-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + an arylamine
UDP + an N-D-glucosylarylamine
show the reaction diagram
-
involved in N-glucosylarylamines biosynthesis, may be participate in arylamine herbicide metabolism
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-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
catechol
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weak
CuSO4
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strong
GSSG
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oxidized glutathione, weak
HgCl2
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strong
iodoacetamide
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weak
o-Iodosobenzoate
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p-benzoquinone
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strong
PCMB
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strong
Sodium arsenate
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1 mM, weak
Tris buffer
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UDP
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linear competitive inhibitor to UDPglucose
additional information
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no inhibition by EDTA and iodoacetate, 1 mM each
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.563
3,4-dichloroaniline
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1.88
UDPglucose
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.484
UDP
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000912
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additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4 - 7.8
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7.5
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with 3,4-dichloroaniline as acceptor
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
additional information
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no activity in cell culture and plant
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
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80% loss of activity within 10 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate at high concentrations inactivates
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freeze-thawing, stable to
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stable to centrifugation at 150000 rpm for 90 min
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ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acetone
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inactivates
Ethanol
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inactivates
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, lyophilized, several months
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4°C, t1/2: 48 h
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freezing for 48 h of plant material causes a slight loss in activity
freezing for 48 h of root material causes 19% loss in activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
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