Information on EC 2.4.1.63 - linamarin synthase

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The expected taxonomic range for this enzyme is: Linum usitatissimum

EC NUMBER
COMMENTARY hide
2.4.1.63
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RECOMMENDED NAME
GeneOntology No.
linamarin synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + 2-hydroxy-2-methylpropanenitrile = UDP + linamarin
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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Cyanoamino acid metabolism
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linamarin biosynthesis
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lotaustralin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:2-hydroxy-2-methylpropanenitrile beta-D-glucosyltransferase
The enzyme glucosylates the cyanohydrins of butanone and pentan-3-one as well as that of acetone.
CAS REGISTRY NUMBER
COMMENTARY hide
37277-68-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
flax
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDPglucose + 2-hydroxy-2-ethylbutanenitrile
UDP + 2-(beta-D-glucopyranosyloxy)-2-ethylbutanenitrile
show the reaction diagram
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-
-
?
UDPglucose + 2-hydroxy-2-methylbutanenitrile
UDP + 2-(beta-D-glucopyranosyloxy)-2-methylbutanenitrile
show the reaction diagram
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-
-
?
UDPglucose + 2-hydroxy-2-methylpropanenitrile
UDP + linamarin
show the reaction diagram
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i.e. acetone cyanohydrin, the cyanohydrins of butanone and pentan-3-one may also be substrates, strict donor specificity, no activity with ADPglucose, CDPglucose, GDPglucose, IDPglucose or TDPglucose
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?
UDPglucose + acetone cyanohydrin
UDP + linamarin
show the reaction diagram
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pathway in cyanogenic glucoside biosynthesis
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDPglucose + acetone cyanohydrin
UDP + linamarin
show the reaction diagram
-
pathway in cyanogenic glucoside biosynthesis
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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no requirement for Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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no requirement for 2-mercaptoethanol or dithiothreitol, 0.01-100 mM
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2 - 9.3
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approx. half-maximal activity at pH 7.2 and 9.3, due to substrate stability, Tris buffer is preferred over glycine-NaOH buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2-mercaptoethanol stabilizes during purification
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
glycerol, ethanol, ammonium sulfate, DTT or albumin does not stabilize during storage at -18°C or room temperature
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
MnCl2, ammonium sulfate, DEAE-cellulose, Sephadex G-100
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